Micro RNA profiles of host extracellular vesicles are modulated by Ascaris suum infection but parasite extracellular vesicle miRNAs are systemically undetectable using in-depth miRNA sequencing

Bradley Whitehead*, Litten Sørensen Rossen, Mads Zippor, Anders T. Boysen, Vineesh Indira Chandran, Per Skallerup, Stig M. Thamsborg, Peter Nejsum*

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

2 Citationer (Scopus)

Abstract

The intestinal helminth Ascaris lumbricoides infects over 800 million people. Infections are often chronic and immunity is not sterilizing due to host-immune modulation, therefore reinfection is common after antihelmintic treatment. We have previously demonstrated a role for Ascaris spp. extracellular vesicles (EVs) in host immune modulation but whether EVs are recognized by the adaptive immune system and are present systemically in the host remains unknown. Therefore, we employed a well-established trickle infection model in pigs to mimic natural Ascaris infection in humans. EVs were isolated from adult Ascaris suum followed by immunoblotting of EV and EV-depleted secretory fractions using plasma from infected and uninfected pigs. Next, EVs were isolated from pig plasma at day 56 post first infection and subjected to deep small RNAseq analysis. RNAs were aligned to A. suum and Sus scrofa miRNA complements to detect A. suum EVs and elucidate the host EV micro RNA (miRNA) response to infection, respectively. Infection generates robust antibody responses against A. suum EVs that is distinct from EV-depleted fractions. However, A. suum miRNAs were not detectable in EVs from the peripheral blood. Notably, host plasma-derived EV miRNA profiles showed significant changes between infected and uninfected pigs, indicating that Ascaris infection drives systemic changes in host EV composition.

OriginalsprogEngelsk
TidsskriftInternational Journal for Parasitology
Vol/bind54
Udgave nummer13
Sider (fra-til)691-696
Antal sider6
ISSN0020-7519
DOI
StatusUdgivet - 2024

Bibliografisk note

Funding Information:
This work was supported by the Danish Council for Independent Research (DFF-1032-00242B). The study was approved by the Animal Experiments Inspectorate, Ministry of Justice, Denmark (Ref. 2010/561-1914). The RNAseq dataset has been deposited to EMBL ArrayExpress with identifier E-MTAB-14168.

Publisher Copyright:
© 2024 The Author(s)

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