Minimal enhancer elements of the leghemoglobin lba and lbc3 gene promoters from Glycine max L. have different properties

Q She; P Lauridsen; J Stougaard; K A Marcker

Minimal enhancer elements of the leghemoglobin lba and lbc3 gene promoters from Glycine max L. have different properties

Q She, P Lauridsen, J Stougaard, K A Marcker

Functional genomics

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

12 Citationer (Scopus)

Abstract

Udgivelsesdato: 1993-Sep

Originalsprog	Engelsk
Tidsskrift	Plant Molecular Biology
Vol/bind	22
Udgave nummer	6
Sider (fra-til)	945-56
Antal sider	11
ISSN	0167-4412
Status	Udgivet - 1993

Bibliografisk note

Keywords: Base Sequence; Conserved Sequence; DNA; Enhancer Elements (Genetics); Genes, Plant; Leghemoglobin; Molecular Sequence Data; Promoter Regions (Genetics); Sequence Deletion; Soybeans

Citationsformater

@article{cbee3ac0c7ad11dd9473000ea68e967b,

title = "Minimal enhancer elements of the leghemoglobin lba and lbc3 gene promoters from Glycine max L. have different properties",

abstract = "The characteristics of the soybean leghemoglobin lba gene promoter were analyzed and important promoter elements from the lba and lbc3 promoters were compared using transgenic Lotus corniculatus plants. A 5' deletion analysis of the lba promoter delimited two cis-acting elements controlling expression: a distal positive element (-1254, -884) required for expression and a proximal element (-285, -60) essential for full-level activity. In contrast to the corresponding region of the lbc3 promoter, the lba proximal element is unable to control expression from the heterologous CaMV 35S enhancer. The upstream positive element of the lba gene contains a position- and orientation-independent enhancer between positions (-1091, -788). The sequence of this enhancer region is conserved in the lbc3 gene upstream (-1333, -1132) of the previously assigned strong positive element (SPE; -1090, -947). The present analysis revealed some of the properties of this extended lbc3 SPE element. The extended element (-1364, -947) functions in both orientations from 5' locations whereas the SPE2 subcomponent (-1364, -1154) containing the conserved sequence is only active in the correct orientation. Removal of the SPE2 by internal deletion demonstrates that the SPE2 subcomponent is indispensable for the activity of the lbc3 upstream positive element. These results indicate that the upstream positive elements of the lba and lbc3 genes possess different properties although their conserved minimal enhancer sequence has similar function. This may reflect the differential expression of the two lb genes of Glycine max L.",

author = "Q She and P Lauridsen and J Stougaard and Marcker, {K A}",

note = "Keywords: Base Sequence; Conserved Sequence; DNA; Enhancer Elements (Genetics); Genes, Plant; Leghemoglobin; Molecular Sequence Data; Promoter Regions (Genetics); Sequence Deletion; Soybeans",

year = "1993",

language = "English",

volume = "22",

pages = "945--56",

journal = "Plant Molecular Biology",

issn = "0167-4412",

publisher = "Springer",

number = "6",

}

TY - JOUR

T1 - Minimal enhancer elements of the leghemoglobin lba and lbc3 gene promoters from Glycine max L. have different properties

AU - She, Q

AU - Lauridsen, P

AU - Stougaard, J

AU - Marcker, K A

N1 - Keywords: Base Sequence; Conserved Sequence; DNA; Enhancer Elements (Genetics); Genes, Plant; Leghemoglobin; Molecular Sequence Data; Promoter Regions (Genetics); Sequence Deletion; Soybeans

PY - 1993

Y1 - 1993

N2 - The characteristics of the soybean leghemoglobin lba gene promoter were analyzed and important promoter elements from the lba and lbc3 promoters were compared using transgenic Lotus corniculatus plants. A 5' deletion analysis of the lba promoter delimited two cis-acting elements controlling expression: a distal positive element (-1254, -884) required for expression and a proximal element (-285, -60) essential for full-level activity. In contrast to the corresponding region of the lbc3 promoter, the lba proximal element is unable to control expression from the heterologous CaMV 35S enhancer. The upstream positive element of the lba gene contains a position- and orientation-independent enhancer between positions (-1091, -788). The sequence of this enhancer region is conserved in the lbc3 gene upstream (-1333, -1132) of the previously assigned strong positive element (SPE; -1090, -947). The present analysis revealed some of the properties of this extended lbc3 SPE element. The extended element (-1364, -947) functions in both orientations from 5' locations whereas the SPE2 subcomponent (-1364, -1154) containing the conserved sequence is only active in the correct orientation. Removal of the SPE2 by internal deletion demonstrates that the SPE2 subcomponent is indispensable for the activity of the lbc3 upstream positive element. These results indicate that the upstream positive elements of the lba and lbc3 genes possess different properties although their conserved minimal enhancer sequence has similar function. This may reflect the differential expression of the two lb genes of Glycine max L.

AB - The characteristics of the soybean leghemoglobin lba gene promoter were analyzed and important promoter elements from the lba and lbc3 promoters were compared using transgenic Lotus corniculatus plants. A 5' deletion analysis of the lba promoter delimited two cis-acting elements controlling expression: a distal positive element (-1254, -884) required for expression and a proximal element (-285, -60) essential for full-level activity. In contrast to the corresponding region of the lbc3 promoter, the lba proximal element is unable to control expression from the heterologous CaMV 35S enhancer. The upstream positive element of the lba gene contains a position- and orientation-independent enhancer between positions (-1091, -788). The sequence of this enhancer region is conserved in the lbc3 gene upstream (-1333, -1132) of the previously assigned strong positive element (SPE; -1090, -947). The present analysis revealed some of the properties of this extended lbc3 SPE element. The extended element (-1364, -947) functions in both orientations from 5' locations whereas the SPE2 subcomponent (-1364, -1154) containing the conserved sequence is only active in the correct orientation. Removal of the SPE2 by internal deletion demonstrates that the SPE2 subcomponent is indispensable for the activity of the lbc3 upstream positive element. These results indicate that the upstream positive elements of the lba and lbc3 genes possess different properties although their conserved minimal enhancer sequence has similar function. This may reflect the differential expression of the two lb genes of Glycine max L.

M3 - Journal article

C2 - 8400139

SN - 0167-4412

VL - 22

SP - 945

EP - 956

JO - Plant Molecular Biology

JF - Plant Molecular Biology

IS - 6

ER -