Modified PAMAM dendrimer with 4-carbomethoxypyrrolidone surface groups reveals negligible toxicity against three rodent cell-lines

Anna Janaszewska, Michal Ciolkowski, Dominika Wróbel, Johannes Fabritius Petersen, Mario Ficker, Jørn Bolstad Christensen, Maria Bryszewska, Barbara Klajnert

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Abstract

Modification of the surface groups of dendrimers is one of the methods to improve their biocompatibility. This article presents results of experiments related to the toxicity of a modified polyamidoamine (PAMAM) dendrimer of the fourth generation with 4-carbomethoxypyrrolidone surface groups (PAMAM-pyrrolidone dendrimer). The cytotoxic activity of the dendrimer was tested on Chinese hamster fibroblasts (B14), embryonic mouse hippocampal cells (mHippoE-18) and rat liver derived cells (BRL-3A). The same cell lines were used to investigate the influence of pyrrolidone dendrimer on the mitochondrial membrane potential, intracellular ROS level and its ability to induce apoptosis or necrosis. The analyzed dendrimer showed only minor toxicity and no ability to induce apoptosis. The most important finding is the lack of influence of the PAMAM-pyrrolidone dendrimer on intracellular ROS level and mitochondrial membrane potential.
OriginalsprogEngelsk
TidsskriftNanomedicine: Nanotechnology, Biology and Medicine
Vol/bind9
Udgave nummer4
Sider (fra-til)461-464
Antal sider4
ISSN1549-9634
DOI
StatusUdgivet - 2013

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