TY - JOUR
T1 - Molecular cloning of a K+ channel from the malaria parasite Plasmodium falciparum
AU - Ellekvist, Peter
AU - Ricke, Christina Høier
AU - Litman, Thomas
AU - Salanti, Ali
AU - Colding, Hanne
AU - Zeuthen, Thomas
AU - Klaerke, Dan A
N1 - Keywords: Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; Conserved Sequence; Erythrocytes; Gene Expression; Humans; Malaria; Membrane Proteins; Molecular Sequence Data; Phylogeny; Plasmodium falciparum; Potassium Channels; Protozoan Proteins; RNA, Messenger; Sequence Alignment
PY - 2004
Y1 - 2004
N2 - In most living cells, K(+) channels are important for the generation of the membrane potential and for volume regulation. The parasite Plasmodium falciparum, which causes malignant malaria, must be able to deal with large variations in the ambient K(+) concentration: it is exposed to high concentrations of K(+) when inside the erythrocyte and low concentrations when in plasma. In the recently published genome of P. falciparum, we have identified a gene, pfkch1, encoding a potential K(+) channel, which to some extent resembles the big-conductance (BK) K(+) channel. We have cloned the approximately 6000 nucleotide (nt) fragment from cDNA, studied the pattern of expression of pfkch1 throughout the intraerythrocytic part of the parasite's life-cyclus, and characterized the channel on the basis of similarity to other K(+) channels from pro- and eukaryotic organisms. This P. falciparum K(+) channel could be a potential drug target.
AB - In most living cells, K(+) channels are important for the generation of the membrane potential and for volume regulation. The parasite Plasmodium falciparum, which causes malignant malaria, must be able to deal with large variations in the ambient K(+) concentration: it is exposed to high concentrations of K(+) when inside the erythrocyte and low concentrations when in plasma. In the recently published genome of P. falciparum, we have identified a gene, pfkch1, encoding a potential K(+) channel, which to some extent resembles the big-conductance (BK) K(+) channel. We have cloned the approximately 6000 nucleotide (nt) fragment from cDNA, studied the pattern of expression of pfkch1 throughout the intraerythrocytic part of the parasite's life-cyclus, and characterized the channel on the basis of similarity to other K(+) channels from pro- and eukaryotic organisms. This P. falciparum K(+) channel could be a potential drug target.
U2 - 10.1016/j.bbrc.2004.04.049
DO - 10.1016/j.bbrc.2004.04.049
M3 - Journal article
C2 - 15120625
SN - 0006-291X
VL - 318
SP - 477
EP - 484
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -