Abstract
Udgivelsesdato: 2005-Feb-4
Originalsprog | Engelsk |
---|---|
Tidsskrift | Biochemical and Biophysical Research Communications |
Vol/bind | 327 |
Udgave nummer | 1 |
Sider (fra-til) | 29-34 |
Antal sider | 5 |
ISSN | 0006-291X |
DOI | |
Status | Udgivet - 2005 |
Bibliografisk note
Keywords: Amino Acid Sequence; Animals; Anopheles gambiae; Base Sequence; CHO Cells; Cloning, Molecular; Cricetinae; DNA, Complementary; Exons; Introns; Molecular Sequence Data; Receptors, Neuropeptide; Sequence AlignmentAdgang til dokumentet
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Molecular identification of a myosuppressin receptor from the malaria mosquito Anopheles gambiae. / Schöller, Susanne; Belmont, Martin; Cazzamali, Giuseppe; Hauser, Frank; Williamson, Michael; Grimmelikhuijzen, Cornelis J P.
I: Biochemical and Biophysical Research Communications, Bind 327, Nr. 1, 2005, s. 29-34.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Molecular identification of a myosuppressin receptor from the malaria mosquito Anopheles gambiae.
AU - Schöller, Susanne
AU - Belmont, Martin
AU - Cazzamali, Giuseppe
AU - Hauser, Frank
AU - Williamson, Michael
AU - Grimmelikhuijzen, Cornelis J P
N1 - Keywords: Amino Acid Sequence; Animals; Anopheles gambiae; Base Sequence; CHO Cells; Cloning, Molecular; Cricetinae; DNA, Complementary; Exons; Introns; Molecular Sequence Data; Receptors, Neuropeptide; Sequence Alignment
PY - 2005
Y1 - 2005
N2 - The insect myosuppressins (X1DVX2HX3FLRFamide) are neuropeptides that generally block insect muscle activities. We have used the genomic sequence information from the malaria mosquito Anopheles gambiae Genome Project to clone a G protein-coupled receptor that was closely related to the two previously cloned and characterized myosuppressin receptors from Drosophila [Proc. Natl. Acad. Sci. USA 100 (2003) 9808]. The mosquito receptor cDNA was expressed in Chinese hamster ovary cells and was found to be activated by low concentrations of Anopheles myosuppressin (TDVDHVFLRFamide; EC50, 1.6 x 10(-8)M). The receptor was not activated by a library of 35 other insect neuropeptides and monoamines, including neuropeptides that resembled myosuppressin in their C-terminal moiety, such as PDRNFLRFamide (Anopheles FMRFamide-3), other Anopheles FMRFamide peptides, or neuropeptide F-like peptides, showing that the receptor was quite selective for myosuppressin. These results also showed that the myosuppressin receptor needs a much larger portion than the C-terminal FLRFamide sequence for its activation. The insect myosuppressins are often grouped together with the insect FMRFamides under the name FaRPs (FMRFamide-related peptides). However, this is not justified anymore, because the insect myosuppressin receptor/ligand couple is both functionally and evolutionarily fully unrelated to the insect FMRFamide receptor/ligand couple. To our knowledge, this is the first report on the molecular identification of a mosquito neuropeptide receptor.
AB - The insect myosuppressins (X1DVX2HX3FLRFamide) are neuropeptides that generally block insect muscle activities. We have used the genomic sequence information from the malaria mosquito Anopheles gambiae Genome Project to clone a G protein-coupled receptor that was closely related to the two previously cloned and characterized myosuppressin receptors from Drosophila [Proc. Natl. Acad. Sci. USA 100 (2003) 9808]. The mosquito receptor cDNA was expressed in Chinese hamster ovary cells and was found to be activated by low concentrations of Anopheles myosuppressin (TDVDHVFLRFamide; EC50, 1.6 x 10(-8)M). The receptor was not activated by a library of 35 other insect neuropeptides and monoamines, including neuropeptides that resembled myosuppressin in their C-terminal moiety, such as PDRNFLRFamide (Anopheles FMRFamide-3), other Anopheles FMRFamide peptides, or neuropeptide F-like peptides, showing that the receptor was quite selective for myosuppressin. These results also showed that the myosuppressin receptor needs a much larger portion than the C-terminal FLRFamide sequence for its activation. The insect myosuppressins are often grouped together with the insect FMRFamides under the name FaRPs (FMRFamide-related peptides). However, this is not justified anymore, because the insect myosuppressin receptor/ligand couple is both functionally and evolutionarily fully unrelated to the insect FMRFamide receptor/ligand couple. To our knowledge, this is the first report on the molecular identification of a mosquito neuropeptide receptor.
U2 - 10.1016/j.bbrc.2004.11.139
DO - 10.1016/j.bbrc.2004.11.139
M3 - Journal article
C2 - 15629425
VL - 327
SP - 29
EP - 34
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -