mRNA profiling for the identification of blood-Results of a collaborative EDNAP exercise

Cordula Haas; E Hanson; W Bär; R Banemann; A M Bento; A Berti; E Borges; C Bouakaze; A Carracedo; M Carvalho; A Choma; M Dötsch; M Durianciková; P Hoff-Olsen; C Hohoff; Peter Johansen; P A Lindenbergh; B Loddenkötter; B Ludes; O Maroñas; N Morling; H Niederstätter; W Parson; G Patel; C Popielarz; E Salata; Peter M. Schneider; T Sijen; B Sviezená; L Zatkalíková; J Ballantyne

doi:10.1016/j.fsigen.2010.01.003

mRNA profiling for the identification of blood-Results of a collaborative EDNAP exercise

Cordula Haas, E Hanson, W Bär, R Banemann, A M Bento, A Berti, E Borges, C Bouakaze, A Carracedo, M Carvalho, A Choma, M Dötsch, M Durianciková, P Hoff-Olsen, C Hohoff, Peter Johansen, P A Lindenbergh, B Loddenkötter, B Ludes, O MaroñasN Morling, H Niederstätter, W Parson, G Patel, C Popielarz, E Salata, Peter M. Schneider, T Sijen, B Sviezená, L Zatkalíková, J Ballantyne

Afdeling for Retsgenetik

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstract

Udgivelsesdato: 2010-Feb-6

Originalsprog	Engelsk
Tidsskrift	Forensic Science International: Genetics
Antal sider	6
ISSN	1872-4973
DOI	https://doi.org/10.1016/j.fsigen.2010.01.003
Status	Udgivet - 2010

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10.1016/j.fsigen.2010.01.003

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Citationsformater

Haas, C., Hanson, E., Bär, W., Banemann, R., Bento, A. M., Berti, A., Borges, E., Bouakaze, C., Carracedo, A., Carvalho, M., Choma, A., Dötsch, M., Durianciková, M., Hoff-Olsen, P., Hohoff, C., Johansen, P., Lindenbergh, P. A., Loddenkötter, B., Ludes, B., ... Ballantyne, J. (2010). mRNA profiling for the identification of blood-Results of a collaborative EDNAP exercise. Forensic Science International: Genetics. https://doi.org/10.1016/j.fsigen.2010.01.003

Haas, C, Hanson, E, Bär, W, Banemann, R, Bento, AM, Berti, A, Borges, E, Bouakaze, C, Carracedo, A, Carvalho, M, Choma, A, Dötsch, M, Durianciková, M, Hoff-Olsen, P, Hohoff, C, Johansen, P, Lindenbergh, PA, Loddenkötter, B, Ludes, B, Maroñas, O, Morling, N, Niederstätter, H, Parson, W, Patel, G, Popielarz, C, Salata, E, Schneider, PM, Sijen, T, Sviezená, B, Zatkalíková, L & Ballantyne, J 2010, 'mRNA profiling for the identification of blood-Results of a collaborative EDNAP exercise', Forensic Science International: Genetics. https://doi.org/10.1016/j.fsigen.2010.01.003

@article{1f154c00b1c411df825b000ea68e967b,

title = "mRNA profiling for the identification of blood-Results of a collaborative EDNAP exercise",

abstract = "A collaborative exercise on mRNA profiling for the identification of blood was organized by the European DNA Profiling Group (EDNAP). Seven blood samples and one blood dilution series were analyzed by the participating laboratories for the reportedly blood-specific markers HBB, SPTB and PBGD, using different kits, chemistries and instrumentation. The results demonstrate that HBB is expressed abundantly in blood, SPTB moderately and PBGD significantly less. All but one of the 16 participating laboratories were able to successfully isolate and detect RNA from the dried bloodstains even though a majority of the laboratories had no prior experience with RNA. Despite some expected variation in sensitivity between laboratories, the method proved to be reproducible and sensitive using different analysis strategies. The results of this collaborative exercise support the potential use of mRNA profiling as an alternative to conventional serological tests.",

author = "Cordula Haas and E Hanson and W B{\"a}r and R Banemann and Bento, {A M} and A Berti and E Borges and C Bouakaze and A Carracedo and M Carvalho and A Choma and M D{\"o}tsch and M Duriancikov{\'a} and P Hoff-Olsen and C Hohoff and Peter Johansen and Lindenbergh, {P A} and B Loddenk{\"o}tter and B Ludes and O Maro{\~n}as and N Morling and H Niederst{\"a}tter and W Parson and G Patel and C Popielarz and E Salata and Schneider, {Peter M.} and T Sijen and B Sviezen{\'a} and L Zatkal{\'i}kov{\'a} and J Ballantyne",

note = "Copyright {\textcopyright} 2010. Published by Elsevier Ireland Ltd.",

year = "2010",

doi = "10.1016/j.fsigen.2010.01.003",

language = "English",

journal = "Forensic Science International: Genetics",

issn = "1872-4973",

publisher = "Elsevier",

}

TY - JOUR

T1 - mRNA profiling for the identification of blood-Results of a collaborative EDNAP exercise

AU - Haas, Cordula

AU - Hanson, E

AU - Bär, W

AU - Banemann, R

AU - Bento, A M

AU - Berti, A

AU - Borges, E

AU - Bouakaze, C

AU - Carracedo, A

AU - Carvalho, M

AU - Choma, A

AU - Dötsch, M

AU - Durianciková, M

AU - Hoff-Olsen, P

AU - Hohoff, C

AU - Johansen, Peter

AU - Lindenbergh, P A

AU - Loddenkötter, B

AU - Ludes, B

AU - Maroñas, O

AU - Morling, N

AU - Niederstätter, H

AU - Parson, W

AU - Patel, G

AU - Popielarz, C

AU - Salata, E

AU - Schneider, Peter M.

AU - Sijen, T

AU - Sviezená, B

AU - Zatkalíková, L

AU - Ballantyne, J

PY - 2010

Y1 - 2010

N2 - A collaborative exercise on mRNA profiling for the identification of blood was organized by the European DNA Profiling Group (EDNAP). Seven blood samples and one blood dilution series were analyzed by the participating laboratories for the reportedly blood-specific markers HBB, SPTB and PBGD, using different kits, chemistries and instrumentation. The results demonstrate that HBB is expressed abundantly in blood, SPTB moderately and PBGD significantly less. All but one of the 16 participating laboratories were able to successfully isolate and detect RNA from the dried bloodstains even though a majority of the laboratories had no prior experience with RNA. Despite some expected variation in sensitivity between laboratories, the method proved to be reproducible and sensitive using different analysis strategies. The results of this collaborative exercise support the potential use of mRNA profiling as an alternative to conventional serological tests.

AB - A collaborative exercise on mRNA profiling for the identification of blood was organized by the European DNA Profiling Group (EDNAP). Seven blood samples and one blood dilution series were analyzed by the participating laboratories for the reportedly blood-specific markers HBB, SPTB and PBGD, using different kits, chemistries and instrumentation. The results demonstrate that HBB is expressed abundantly in blood, SPTB moderately and PBGD significantly less. All but one of the 16 participating laboratories were able to successfully isolate and detect RNA from the dried bloodstains even though a majority of the laboratories had no prior experience with RNA. Despite some expected variation in sensitivity between laboratories, the method proved to be reproducible and sensitive using different analysis strategies. The results of this collaborative exercise support the potential use of mRNA profiling as an alternative to conventional serological tests.

U2 - 10.1016/j.fsigen.2010.01.003

DO - 10.1016/j.fsigen.2010.01.003

M3 - Journal article

C2 - 20457073

SN - 1872-4973

JO - Forensic Science International: Genetics

JF - Forensic Science International: Genetics

ER -