Mucosal expression of PI3, ANXA1, and VDR discriminates Crohn’s disease from ulcerative colitis

Jaslin Pallikkunnath James*, Boye Schnack Nielsen, Ib Jarle Christensen, Ebbe Langholz, Mikkel Malham, Tim Svenstrup Poulsen, Kim Holmstrøm, Lene Buhl Riis, Estrid Høgdall

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

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Abstract

Differential diagnosis of inflammatory bowel disease (IBD) to Crohn’s disease (CD) or ulcerative colitis (UC) is crucial for treatment decision making. With the aim of generating a clinically applicable molecular-based tool to classify IBD patients, we assessed whole transcriptome analysis on endoscopy samples. A total of 408 patient samples were included covering both internal and external samples cohorts. Whole transcriptome analysis was performed on an internal cohort of FFPE IBD samples (CD, n = 16 and UC, n = 17). The 100 most significantly differentially expressed genes (DEG) were tested in two external cohorts. Ten of the DEG were further processed by functional enrichment analysis from which seven were found to show consistent significant performance in discriminating CD from UC: PI3, ANXA1, VDR, MTCL1, SH3PXD2A-AS1, CLCF1, and CD180. Differential expression of PI3, ANXA1, and VDR was reproduced by RT-qPCR, which was performed on an independent sample cohort of 97 patient samples (CD, n = 44 and UC, n = 53). Gene expression levels of the three-gene profile, resulted in an area under the curve of 0.84 (P = 0.02) in discriminating CD from UC, and therefore appear as an attractive molecular-based diagnostic tool for clinicians to distinguish CD from UC.
OriginalsprogEngelsk
Artikelnummer18421
TidsskriftScientific Reports
Vol/bind13
Udgave nummer1
Antal sider13
ISSN2045-2322
DOI
StatusUdgivet - 2023

Bibliografisk note

Funding Information:
This work was supported by Aage Louis Hansen fonden [J.nr.20-2B-5995]; Herlev internal research funding [after year, 2019]; Colitis-Crohn Foreningen [2021]; Torben og Alice Frimodts Fond [FK1900]; Aase and Ejnar Danielsen's Foundation [20-10-0411].

Publisher Copyright:
© 2023, The Author(s).

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