Multiple antioxidant proteins protect Chlorobaculum tepidum against oxygen and reactive oxygen species

Hui Li; Sara Jubelirer; Amaya M Garcia Costas; Niels-Ulrik Frigaard; Donald A Bryant

doi:10.1007/s00203-009-0514-7

Multiple antioxidant proteins protect Chlorobaculum tepidum against oxygen and reactive oxygen species

Hui Li, Sara Jubelirer, Amaya M Garcia Costas, Niels-Ulrik Frigaard, Donald A Bryant

Biomolecular Sciences

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

33 Citationer (Scopus)

Abstract

Udgivelsesdato: 2009-Nov

Originalsprog	Engelsk
Tidsskrift	Archives of Microbiology
Vol/bind	191
Udgave nummer	11
Sider (fra-til)	853-67
Antal sider	14
ISSN	0302-8933
DOI	https://doi.org/10.1007/s00203-009-0514-7
Status	Udgivet - 2009

Bibliografisk note

Keywords Green sulfur bacteria - Oxidative stress - Chlorobaculum tepidum - Photosynthesis

Adgang til dokumentet

10.1007/s00203-009-0514-7

Citationsformater

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title = "Multiple antioxidant proteins protect Chlorobaculum tepidum against oxygen and reactive oxygen species",

abstract = "The genome of the green sulfur bacterium Chlorobaculum (Cba.) tepidum, a strictly anaerobic photolithoautotroph, is predicted to encode more than ten genes whose products are potentially involved in protection from reactive oxygen species and an oxidative stress response. The encoded proteins include cytochrome bd quinol oxidase, NADH oxidase, rubredoxin oxygen oxidoreductase, several thiol peroxidases, alkyl hydroperoxide reductase, superoxide dismutase, methionine sulfoxide reductase, and rubrerythrin. To test the physiological functions of some of these proteins, ten genes were insertionally inactivated. Wild-type Cba. tepidum cells were very sensitive to oxygen in the light but were remarkably resistant to oxygen in the dark. When wild-type and mutant cells were subjected to air for various times under dark or light condition, significant decreases in viability were detected in most of the mutants relative to wild type. Treatments with hydrogen peroxide (H(2)O(2)), tert-butyl hydroperoxide (t-BOOH) and methyl viologen resulted in more severe effects in most of the mutants than in the wild type. The results demonstrated that these putative antioxidant proteins combine to form an effective defense against oxygen and reactive oxygen species. Reverse-transcriptase polymerase chain reaction studies showed that the genes with functions in oxidative stress protection were constitutively transcribed under anoxic growth conditions.",

author = "Hui Li and Sara Jubelirer and {Garcia Costas}, {Amaya M} and Niels-Ulrik Frigaard and Bryant, {Donald A}",

note = "Keywords Green sulfur bacteria - Oxidative stress - Chlorobaculum tepidum - Photosynthesis",

year = "2009",

doi = "10.1007/s00203-009-0514-7",

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TY - JOUR

T1 - Multiple antioxidant proteins protect Chlorobaculum tepidum against oxygen and reactive oxygen species

AU - Li, Hui

AU - Jubelirer, Sara

AU - Garcia Costas, Amaya M

AU - Frigaard, Niels-Ulrik

AU - Bryant, Donald A

N1 - Keywords Green sulfur bacteria - Oxidative stress - Chlorobaculum tepidum - Photosynthesis

PY - 2009

Y1 - 2009

N2 - The genome of the green sulfur bacterium Chlorobaculum (Cba.) tepidum, a strictly anaerobic photolithoautotroph, is predicted to encode more than ten genes whose products are potentially involved in protection from reactive oxygen species and an oxidative stress response. The encoded proteins include cytochrome bd quinol oxidase, NADH oxidase, rubredoxin oxygen oxidoreductase, several thiol peroxidases, alkyl hydroperoxide reductase, superoxide dismutase, methionine sulfoxide reductase, and rubrerythrin. To test the physiological functions of some of these proteins, ten genes were insertionally inactivated. Wild-type Cba. tepidum cells were very sensitive to oxygen in the light but were remarkably resistant to oxygen in the dark. When wild-type and mutant cells were subjected to air for various times under dark or light condition, significant decreases in viability were detected in most of the mutants relative to wild type. Treatments with hydrogen peroxide (H(2)O(2)), tert-butyl hydroperoxide (t-BOOH) and methyl viologen resulted in more severe effects in most of the mutants than in the wild type. The results demonstrated that these putative antioxidant proteins combine to form an effective defense against oxygen and reactive oxygen species. Reverse-transcriptase polymerase chain reaction studies showed that the genes with functions in oxidative stress protection were constitutively transcribed under anoxic growth conditions.

AB - The genome of the green sulfur bacterium Chlorobaculum (Cba.) tepidum, a strictly anaerobic photolithoautotroph, is predicted to encode more than ten genes whose products are potentially involved in protection from reactive oxygen species and an oxidative stress response. The encoded proteins include cytochrome bd quinol oxidase, NADH oxidase, rubredoxin oxygen oxidoreductase, several thiol peroxidases, alkyl hydroperoxide reductase, superoxide dismutase, methionine sulfoxide reductase, and rubrerythrin. To test the physiological functions of some of these proteins, ten genes were insertionally inactivated. Wild-type Cba. tepidum cells were very sensitive to oxygen in the light but were remarkably resistant to oxygen in the dark. When wild-type and mutant cells were subjected to air for various times under dark or light condition, significant decreases in viability were detected in most of the mutants relative to wild type. Treatments with hydrogen peroxide (H(2)O(2)), tert-butyl hydroperoxide (t-BOOH) and methyl viologen resulted in more severe effects in most of the mutants than in the wild type. The results demonstrated that these putative antioxidant proteins combine to form an effective defense against oxygen and reactive oxygen species. Reverse-transcriptase polymerase chain reaction studies showed that the genes with functions in oxidative stress protection were constitutively transcribed under anoxic growth conditions.

U2 - 10.1007/s00203-009-0514-7

DO - 10.1007/s00203-009-0514-7

M3 - Journal article

C2 - 19784828

VL - 191

SP - 853

EP - 867

JO - Archives of Microbiology

JF - Archives of Microbiology

SN - 0302-8933

IS - 11

ER -