Natural history of choroidal neovascularization after surgical induction in an animal model

Nathan Lassota; Jens Folke Kiilgaard; Morten la Cour; Erik Scherfig; Jan Ulrik Prause; Nathan Lassota; Jens Folke Kiilgaard; Morten la Cour; Erik Scherfig; Jan Ulrik Prause

doi:10.1111/j.1600-0420.2007.01127.x

Natural history of choroidal neovascularization after surgical induction in an animal model

Nathan Lassota, Jens Folke Kiilgaard, Morten la Cour, Erik Scherfig, Jan Ulrik Prause, Nathan Lassota, Jens Folke Kiilgaard, Morten la Cour, Erik Scherfig, Jan Ulrik Prause

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

22 Citationer (Scopus)

Abstract

Udgivelsesdato: 2008-Aug

Originalsprog	Engelsk
Tidsskrift	Acta Ophthamologica (Online)
Vol/bind	86
Udgave nummer	5
Sider (fra-til)	495-503
Antal sider	9
ISSN	1755-3768
DOI	https://doi.org/10.1111/j.1600-0420.2007.01127.x https://doi.org/10.1111/j.1600-0420.2007.01127.x
Status	Udgivet - 2008

Bibliografisk note

Keywords: Actins; Animals; Blood-Retinal Barrier; Bruch Membrane; Capillary Permeability; Choroidal Neovascularization; Collagen; Disease Models, Animal; Female; Fluorescein Angiography; Glial Fibrillary Acidic Protein; Immunoenzyme Techniques; Macrophages; Muramidase; Retinal Pigment Epithelium; Swine; Vitrectomy; von Willebrand Factor

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Citationsformater

Lassota, N., Kiilgaard, J. F., la Cour, M., Scherfig, E., Prause, J. U., Lassota, N., Kiilgaard, J. F., la Cour, M., Scherfig, E., & Prause, J. U. (2008). Natural history of choroidal neovascularization after surgical induction in an animal model. Acta Ophthamologica (Online), 86(5), 495-503. https://doi.org/10.1111/j.1600-0420.2007.01127.x, https://doi.org/10.1111/j.1600-0420.2007.01127.x

Natural history of choroidal neovascularization after surgical induction in an animal model. / Lassota, Nathan; Kiilgaard, Jens Folke; la Cour, Morten; Scherfig, Erik; Prause, Jan Ulrik; Lassota, Nathan; Kiilgaard, Jens Folke ; la Cour, Morten; Scherfig, Erik; Prause, Jan Ulrik.

I: Acta Ophthamologica (Online), Bind 86, Nr. 5, 2008, s. 495-503.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Lassota, N, Kiilgaard, JF, la Cour, M, Scherfig, E, Prause, JU, Lassota, N, Kiilgaard, JF , la Cour, M, Scherfig, E & Prause, JU 2008, 'Natural history of choroidal neovascularization after surgical induction in an animal model', Acta Ophthamologica (Online), bind 86, nr. 5, s. 495-503. https://doi.org/10.1111/j.1600-0420.2007.01127.x, https://doi.org/10.1111/j.1600-0420.2007.01127.x

@article{88b91800f90411ddb219000ea68e967b,

title = "Natural history of choroidal neovascularization after surgical induction in an animal model",

abstract = "PURPOSE: To study an expanded time course of surgically induced choroidal neovascularization (CNV) in a porcine model applying fluorescence angiography and immunohistology. METHODS: Twenty-two porcine eyes underwent vitrectomy, a retinal bleb was raised and the detached retina perforated using endodiathermy. Bruch's membrane was perforated with a retinal perforator at a site where the overlying neuroretina was normal. Eyes were enucleated in a time interval between 30 min and 42 days after the perforation, and the pigs were subsequently killed. Immediately prior to enucleation, fundus photographs and fluorescein angiograms were obtained. Sections of paraffin-embedded eyes were immunohistochemically stained. RESULTS: On fluorescein angiography, membranes aged 14 days or less exhibited leakage in 10/11 cases while the remaining showed persistent staining. The propensity to leak diminished with time and only 1/3 of the oldest membranes leaked. In eyes enucleated immediately after surgery, neuroretinas overlying the induced lesions were intact without apparent atrophy of cells. At day 3, macrophages and myofibroblasts formed membrane-like structures in the subretinal space. At day 7, the outer surface of the membrane was covered by retinal pigment epithelium (RPE) cells and the neuroretinas had suffered damage in the form of outer segment loss. In the time period 14-42 days, the CNV membrane became completely enveloped by RPE cells. The degree of membrane vascularization increased with time and was at its maximum after 42 days. Intact outer segments were identified over the oldest membranes. CONCLUSION: The formation of surgical CNV membranes followed the normal reparatory pathway and the degree of vascularization of CNV membranes continued to increase during the 42 days. However, propensity to leak diminished with time. We believe that this was because of the fact that RPE cells completely enveloped older membrane and thus prevented leakage from the newly formed vessels. Photoreceptor outer segments, which had atrophied after 7 days, were able to regenerate over CNV membranes and could be identified again after 42 days.",

author = "Nathan Lassota and Kiilgaard, {Jens Folke} and {la Cour}, Morten and Erik Scherfig and Prause, {Jan Ulrik} and Nathan Lassota and Kiilgaard, {Jens Folke} and {la Cour}, Morten and Erik Scherfig and Prause, {Jan Ulrik}",

note = "Keywords: Actins; Animals; Blood-Retinal Barrier; Bruch Membrane; Capillary Permeability; Choroidal Neovascularization; Collagen; Disease Models, Animal; Female; Fluorescein Angiography; Glial Fibrillary Acidic Protein; Immunoenzyme Techniques; Macrophages; Muramidase; Retinal Pigment Epithelium; Swine; Vitrectomy; von Willebrand Factor",

year = "2008",

doi = "10.1111/j.1600-0420.2007.01127.x",

language = "English",

volume = "86",

pages = "495--503",

journal = "Acta Ophthalmologica",

issn = "1755-375X",

publisher = "Wiley-Blackwell",

number = "5",

}

TY - JOUR

T1 - Natural history of choroidal neovascularization after surgical induction in an animal model

AU - Lassota, Nathan

AU - Kiilgaard, Jens Folke

AU - la Cour, Morten

AU - Scherfig, Erik

AU - Prause, Jan Ulrik

AU - Lassota, Nathan

AU - Kiilgaard, Jens Folke

AU - la Cour, Morten

AU - Scherfig, Erik

AU - Prause, Jan Ulrik

N1 - Keywords: Actins; Animals; Blood-Retinal Barrier; Bruch Membrane; Capillary Permeability; Choroidal Neovascularization; Collagen; Disease Models, Animal; Female; Fluorescein Angiography; Glial Fibrillary Acidic Protein; Immunoenzyme Techniques; Macrophages; Muramidase; Retinal Pigment Epithelium; Swine; Vitrectomy; von Willebrand Factor

PY - 2008

Y1 - 2008

N2 - PURPOSE: To study an expanded time course of surgically induced choroidal neovascularization (CNV) in a porcine model applying fluorescence angiography and immunohistology. METHODS: Twenty-two porcine eyes underwent vitrectomy, a retinal bleb was raised and the detached retina perforated using endodiathermy. Bruch's membrane was perforated with a retinal perforator at a site where the overlying neuroretina was normal. Eyes were enucleated in a time interval between 30 min and 42 days after the perforation, and the pigs were subsequently killed. Immediately prior to enucleation, fundus photographs and fluorescein angiograms were obtained. Sections of paraffin-embedded eyes were immunohistochemically stained. RESULTS: On fluorescein angiography, membranes aged 14 days or less exhibited leakage in 10/11 cases while the remaining showed persistent staining. The propensity to leak diminished with time and only 1/3 of the oldest membranes leaked. In eyes enucleated immediately after surgery, neuroretinas overlying the induced lesions were intact without apparent atrophy of cells. At day 3, macrophages and myofibroblasts formed membrane-like structures in the subretinal space. At day 7, the outer surface of the membrane was covered by retinal pigment epithelium (RPE) cells and the neuroretinas had suffered damage in the form of outer segment loss. In the time period 14-42 days, the CNV membrane became completely enveloped by RPE cells. The degree of membrane vascularization increased with time and was at its maximum after 42 days. Intact outer segments were identified over the oldest membranes. CONCLUSION: The formation of surgical CNV membranes followed the normal reparatory pathway and the degree of vascularization of CNV membranes continued to increase during the 42 days. However, propensity to leak diminished with time. We believe that this was because of the fact that RPE cells completely enveloped older membrane and thus prevented leakage from the newly formed vessels. Photoreceptor outer segments, which had atrophied after 7 days, were able to regenerate over CNV membranes and could be identified again after 42 days.

AB - PURPOSE: To study an expanded time course of surgically induced choroidal neovascularization (CNV) in a porcine model applying fluorescence angiography and immunohistology. METHODS: Twenty-two porcine eyes underwent vitrectomy, a retinal bleb was raised and the detached retina perforated using endodiathermy. Bruch's membrane was perforated with a retinal perforator at a site where the overlying neuroretina was normal. Eyes were enucleated in a time interval between 30 min and 42 days after the perforation, and the pigs were subsequently killed. Immediately prior to enucleation, fundus photographs and fluorescein angiograms were obtained. Sections of paraffin-embedded eyes were immunohistochemically stained. RESULTS: On fluorescein angiography, membranes aged 14 days or less exhibited leakage in 10/11 cases while the remaining showed persistent staining. The propensity to leak diminished with time and only 1/3 of the oldest membranes leaked. In eyes enucleated immediately after surgery, neuroretinas overlying the induced lesions were intact without apparent atrophy of cells. At day 3, macrophages and myofibroblasts formed membrane-like structures in the subretinal space. At day 7, the outer surface of the membrane was covered by retinal pigment epithelium (RPE) cells and the neuroretinas had suffered damage in the form of outer segment loss. In the time period 14-42 days, the CNV membrane became completely enveloped by RPE cells. The degree of membrane vascularization increased with time and was at its maximum after 42 days. Intact outer segments were identified over the oldest membranes. CONCLUSION: The formation of surgical CNV membranes followed the normal reparatory pathway and the degree of vascularization of CNV membranes continued to increase during the 42 days. However, propensity to leak diminished with time. We believe that this was because of the fact that RPE cells completely enveloped older membrane and thus prevented leakage from the newly formed vessels. Photoreceptor outer segments, which had atrophied after 7 days, were able to regenerate over CNV membranes and could be identified again after 42 days.

U2 - 10.1111/j.1600-0420.2007.01127.x

DO - 10.1111/j.1600-0420.2007.01127.x

M3 - Journal article

C2 - 18752525

VL - 86

SP - 495

EP - 503

JO - Acta Ophthalmologica

JF - Acta Ophthalmologica

SN - 1755-375X

IS - 5

ER -