@article{284b31305ede11dd8d9f000ea68e967b,
title = "Neuroblastoma and pre-B lymphoma cells share expression of key transcription factors but display tissue restricted target gene expression.",
abstract = "BACKGROUND: Transcription factors are frequently involved in the process of cellular transformation, and many malignancies are characterized by a distinct genetic event affecting a specific transcription factor. This probably reflects a tissue specific ability of transcription factors to contribute to the generation of cancer but very little is known about the precise mechanisms that governs these restricted effects. METHODS: To investigate this selectivity in target gene activation we compared the overall gene expression patterns by micro-array analysis and expression of target genes for the transcription factor EBF in lymphoma and neuroblastoma cells by RT-PCR. The presence of transcription factors in the different model cell lines was further investigated by EMSA analysis. RESULTS: In pre-B cells mb-1 and CD19 are regulate by EBF-1 in collaboration with Pax-5 and E-proteins. We here show that neuroblastoma cells express these three, for B cell development crucial transcription factors, but nevertheless fail to express detectable levels of their known target genes. Expression of mb-1 could, however, be induced in neuroblastoma cells after disruption of the chromatin structure by treatment with 5-azacytidine and Trichostatin A. CONCLUSION: These data suggest that transcription factors are able to selectively activate target genes in different tissues and that chromatin structure plays a key role in the regulation of this activity.",
author = "Anna Lagergren and Manetopulos, {Anna Kristina} and H{\aa}kan Axelson and Mikael Sigvardsson",
note = "Keywords: Antigens, CD; Antigens, CD19; Antigens, CD79; B-Cell-Specific Activator Protein; B-Lymphocytes; Cells, Cultured; DNA-Binding Proteins; HMGB Proteins; Humans; Lymphoma, B-Cell; Neuroblastoma; Oligonucleotide Array Sequence Analysis; Receptors, Antigen, B-Cell; Reverse Transcriptase Polymerase Chain Reaction; TCF Transcription Factors; Trans-Activators; Transcription Factors",
year = "2004",
doi = "10.1186/1471-2407-4-80",
language = "English",
volume = "4",
pages = "80",
journal = "B M C Cancer",
issn = "1471-2407",
publisher = "BioMed Central Ltd.",
}