NMR-Based Metabonomic Investigation of Heat Stress in Myotubes Reveals a Time-Dependent Change in the Metabolites

Ida K Straadt; Jette F Young; Peter Bross; Niels Gregersen; Niels Oksbjerg; Peter K Theil; Hanne C Bertram

doi:10.1021/jf904197u

NMR-Based Metabonomic Investigation of Heat Stress in Myotubes Reveals a Time-Dependent Change in the Metabolites

Ida K Straadt, Jette F Young, Peter Bross, Niels Gregersen, Niels Oksbjerg, Peter K Theil, Hanne C Bertram

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

7 Citationer (Scopus)

Abstract

Udgivelsesdato: 2010-Apr-29

Originalsprog	Engelsk
Tidsskrift	Journal of Agricultural and Food Chemistry
Vol/bind	58
Sider (fra-til)	6376-6386
Antal sider	11
ISSN	0021-8561
DOI	https://doi.org/10.1021/jf904197u
Status	Udgivet - 2010
Udgivet eksternt	Ja

Adgang til dokumentet

10.1021/jf904197u

Citationsformater

NMR-Based Metabonomic Investigation of Heat Stress in Myotubes Reveals a Time-Dependent Change in the Metabolites. / Straadt, Ida K; Young, Jette F; Bross, Peter; Gregersen, Niels; Oksbjerg, Niels; Theil, Peter K; Bertram, Hanne C.

I: Journal of Agricultural and Food Chemistry, Bind 58, 2010, s. 6376-6386.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

@article{3049559075c145e6a4fbe2bf4f0eb09e,

title = "NMR-Based Metabonomic Investigation of Heat Stress in Myotubes Reveals a Time-Dependent Change in the Metabolites",

abstract = "NMR-based metabonomics was applied to elucidate the time-dependent stress responses in mouse myotubes after heat exposure of either 42 or 45 degrees C for 1 h. Principal component analysis (PCA) revealed that the gradual time-dependent changes in metabolites contributing to the clustering and separation of the control samples from the different time points after heat stress primarily are in the metabolites glucose, leucine, lysine, phenylalanine, creatine, glutamine, and acetate. In addition, PC scores revealed a maximum change in metabolite composition 4 h after the stress exposure; thereafter, samples returned toward control samples, however, without reaching the control samples even 10 h after stress. The results also indicate that the myotubes efficiently regulate the pH level by release of lactate to the culture medium at a heat stress level of 42 degrees C, which is a temperature level reached in muscles of pigs during exposure to slaughter stress.",

author = "Straadt, {Ida K} and Young, {Jette F} and Peter Bross and Niels Gregersen and Niels Oksbjerg and Theil, {Peter K} and Bertram, {Hanne C}",

year = "2010",

doi = "10.1021/jf904197u",

language = "English",

volume = "58",

pages = "6376--6386",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "American Chemical Society",

}

TY - JOUR

T1 - NMR-Based Metabonomic Investigation of Heat Stress in Myotubes Reveals a Time-Dependent Change in the Metabolites

AU - Straadt, Ida K

AU - Young, Jette F

AU - Bross, Peter

AU - Gregersen, Niels

AU - Oksbjerg, Niels

AU - Theil, Peter K

AU - Bertram, Hanne C

PY - 2010

Y1 - 2010

N2 - NMR-based metabonomics was applied to elucidate the time-dependent stress responses in mouse myotubes after heat exposure of either 42 or 45 degrees C for 1 h. Principal component analysis (PCA) revealed that the gradual time-dependent changes in metabolites contributing to the clustering and separation of the control samples from the different time points after heat stress primarily are in the metabolites glucose, leucine, lysine, phenylalanine, creatine, glutamine, and acetate. In addition, PC scores revealed a maximum change in metabolite composition 4 h after the stress exposure; thereafter, samples returned toward control samples, however, without reaching the control samples even 10 h after stress. The results also indicate that the myotubes efficiently regulate the pH level by release of lactate to the culture medium at a heat stress level of 42 degrees C, which is a temperature level reached in muscles of pigs during exposure to slaughter stress.

AB - NMR-based metabonomics was applied to elucidate the time-dependent stress responses in mouse myotubes after heat exposure of either 42 or 45 degrees C for 1 h. Principal component analysis (PCA) revealed that the gradual time-dependent changes in metabolites contributing to the clustering and separation of the control samples from the different time points after heat stress primarily are in the metabolites glucose, leucine, lysine, phenylalanine, creatine, glutamine, and acetate. In addition, PC scores revealed a maximum change in metabolite composition 4 h after the stress exposure; thereafter, samples returned toward control samples, however, without reaching the control samples even 10 h after stress. The results also indicate that the myotubes efficiently regulate the pH level by release of lactate to the culture medium at a heat stress level of 42 degrees C, which is a temperature level reached in muscles of pigs during exposure to slaughter stress.

U2 - 10.1021/jf904197u

DO - 10.1021/jf904197u

M3 - Journal article

C2 - 20429597

VL - 58

SP - 6376

EP - 6386

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

ER -