Nonmucoid Pseudomonas aeruginosa expresses alginate in the lungs of patients with cystic fibrosis and in a mouse model

Alessandra Bragonzi; Dieter Worlitzsch; Gerald B Pier; Petra Timpert; Martina Ulrich; Morten Hentzer; Jens Bo Andersen; Michael Givskov; Massimo Conese; Gerd Doring

doi:10.1086/431516

Nonmucoid Pseudomonas aeruginosa expresses alginate in the lungs of patients with cystic fibrosis and in a mouse model

Alessandra Bragonzi, Dieter Worlitzsch, Gerald B Pier, Petra Timpert, Martina Ulrich, Morten Hentzer, Jens Bo Andersen, Michael Givskov, Massimo Conese, Gerd Doring

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

125 Citationer (Scopus)

Abstract

Udgivelsesdato: 2005-Aug-1

Originalsprog	Engelsk
Tidsskrift	Journal of Infectious Diseases
Vol/bind	192
Udgave nummer	3
Sider (fra-til)	410-9
Antal sider	9
ISSN	0022-1899
DOI	https://doi.org/10.1086/431516
Status	Udgivet - 2005
Udgivet eksternt	Ja

Bibliografisk note

Keywords: Alginates; Animals; Bacteroides fragilis; Cystic Fibrosis; Disease Models, Animal; Gene Expression Regulation; Humans; Lung; Mice; Pseudomonas aeruginosa; Sputum

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Citationsformater

@article{af699090fcee11ddb219000ea68e967b,

title = "Nonmucoid Pseudomonas aeruginosa expresses alginate in the lungs of patients with cystic fibrosis and in a mouse model",

abstract = "BACKGROUND: In patients with cystic fibrosis (CF), lung infection with mucoid Pseudomonas aeruginosa strains overexpressing the exopolysaccaride alginate is preceded by colonization with nonmucoid strains. We investigated the kinetics, impact of environmental signals, and genetics of P. aeruginosa alginate expression in a mouse model and in patients with CF. METHODS: Using indirect immunofluorescence, microarray technology and real-time reverse-transcription polymerase chain reaction, we assessed alginate gene expression during aerobic and anaerobic growth of the nonmucoid strain PAO1 in vitro, in a mouse lung-infection model and in sputum specimens from patients with CF infected with nonmucoid or mucoid P. aeruginosa strains. RESULTS: Anaerobic conditions increased the transcription of alginate genes in vitro and in murine lungs within 24 h. Alginate production by PAO1 in murine lungs and by nonmucoid P. aeruginosa strains in patients with CF was reversible after in vitro culture under aerobic conditions. A subpopulation of P. aeruginosa clones revealing stable alginate production was detected in murine lungs 2 weeks after infection. CONCLUSIONS: Anaerobiosis and lung infection rapidly induce alginate production by gene regulation in nonmucoid P. aeruginosa. This trait may contribute to early persistence, leading to chronic P. aeruginosa infection once stable mucoid strains are generated.",

author = "Alessandra Bragonzi and Dieter Worlitzsch and Pier, {Gerald B} and Petra Timpert and Martina Ulrich and Morten Hentzer and Andersen, {Jens Bo} and Michael Givskov and Massimo Conese and Gerd Doring",

note = "Keywords: Alginates; Animals; Bacteroides fragilis; Cystic Fibrosis; Disease Models, Animal; Gene Expression Regulation; Humans; Lung; Mice; Pseudomonas aeruginosa; Sputum",

year = "2005",

doi = "10.1086/431516",

language = "English",

volume = "192",

pages = "410--9",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

number = "3",

}

TY - JOUR

T1 - Nonmucoid Pseudomonas aeruginosa expresses alginate in the lungs of patients with cystic fibrosis and in a mouse model

AU - Bragonzi, Alessandra

AU - Worlitzsch, Dieter

AU - Pier, Gerald B

AU - Timpert, Petra

AU - Ulrich, Martina

AU - Hentzer, Morten

AU - Andersen, Jens Bo

AU - Givskov, Michael

AU - Conese, Massimo

AU - Doring, Gerd

N1 - Keywords: Alginates; Animals; Bacteroides fragilis; Cystic Fibrosis; Disease Models, Animal; Gene Expression Regulation; Humans; Lung; Mice; Pseudomonas aeruginosa; Sputum

PY - 2005

Y1 - 2005

N2 - BACKGROUND: In patients with cystic fibrosis (CF), lung infection with mucoid Pseudomonas aeruginosa strains overexpressing the exopolysaccaride alginate is preceded by colonization with nonmucoid strains. We investigated the kinetics, impact of environmental signals, and genetics of P. aeruginosa alginate expression in a mouse model and in patients with CF. METHODS: Using indirect immunofluorescence, microarray technology and real-time reverse-transcription polymerase chain reaction, we assessed alginate gene expression during aerobic and anaerobic growth of the nonmucoid strain PAO1 in vitro, in a mouse lung-infection model and in sputum specimens from patients with CF infected with nonmucoid or mucoid P. aeruginosa strains. RESULTS: Anaerobic conditions increased the transcription of alginate genes in vitro and in murine lungs within 24 h. Alginate production by PAO1 in murine lungs and by nonmucoid P. aeruginosa strains in patients with CF was reversible after in vitro culture under aerobic conditions. A subpopulation of P. aeruginosa clones revealing stable alginate production was detected in murine lungs 2 weeks after infection. CONCLUSIONS: Anaerobiosis and lung infection rapidly induce alginate production by gene regulation in nonmucoid P. aeruginosa. This trait may contribute to early persistence, leading to chronic P. aeruginosa infection once stable mucoid strains are generated.

AB - BACKGROUND: In patients with cystic fibrosis (CF), lung infection with mucoid Pseudomonas aeruginosa strains overexpressing the exopolysaccaride alginate is preceded by colonization with nonmucoid strains. We investigated the kinetics, impact of environmental signals, and genetics of P. aeruginosa alginate expression in a mouse model and in patients with CF. METHODS: Using indirect immunofluorescence, microarray technology and real-time reverse-transcription polymerase chain reaction, we assessed alginate gene expression during aerobic and anaerobic growth of the nonmucoid strain PAO1 in vitro, in a mouse lung-infection model and in sputum specimens from patients with CF infected with nonmucoid or mucoid P. aeruginosa strains. RESULTS: Anaerobic conditions increased the transcription of alginate genes in vitro and in murine lungs within 24 h. Alginate production by PAO1 in murine lungs and by nonmucoid P. aeruginosa strains in patients with CF was reversible after in vitro culture under aerobic conditions. A subpopulation of P. aeruginosa clones revealing stable alginate production was detected in murine lungs 2 weeks after infection. CONCLUSIONS: Anaerobiosis and lung infection rapidly induce alginate production by gene regulation in nonmucoid P. aeruginosa. This trait may contribute to early persistence, leading to chronic P. aeruginosa infection once stable mucoid strains are generated.

U2 - 10.1086/431516

DO - 10.1086/431516

M3 - Journal article

C2 - 15995954

SN - 0022-1899

VL - 192

SP - 410

EP - 419

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

IS - 3

ER -