Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Journal of Neuroscience Research |
| Vol/bind | 84 |
| Udgave nummer | 7 |
| Sider (fra-til) | 1452-74 |
| Antal sider | 22 |
| ISSN | 0360-4012 |
| DOI | |
| Status | Udgivet - 2006 |
Bibliografisk note
Keywords: Analysis of Variance; Animals; Aquaporin 2; Brain Injuries; Catalase; Cluster Analysis; Gene Expression Profiling; Gene Expression Regulation; Immunohistochemistry; Metallothionein; Mice; Mice, Knockout; Mitogen-Activated Protein Kinases; Oligonucleotide Array Sequence Analysis; Stem Cells; Time FactorsAdgang til dokumentet
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I: Journal of Neuroscience Research, Bind 84, Nr. 7, 2006, s. 1452-74.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Novel roles for metallothionein-I + II (MT-I + II) in defense responses, neurogenesis, and tissue restoration after traumatic brain injury: insights from global gene expression profiling in wild-type and MT-I + II knockout mice
AU - Penkowa, Milena
AU - Cáceres, Mario
AU - Borup, Rehannah
AU - Nielsen, Finn Cilius
AU - Poulsen, Christian Bjørn
AU - Quintana, Albert
AU - Molinero, Amalia
AU - Carrasco, Javier
AU - Florit, Sergi
AU - Giralt, Mercedes
AU - Hidalgo, Juan
N1 - Keywords: Analysis of Variance; Animals; Aquaporin 2; Brain Injuries; Catalase; Cluster Analysis; Gene Expression Profiling; Gene Expression Regulation; Immunohistochemistry; Metallothionein; Mice; Mice, Knockout; Mitogen-Activated Protein Kinases; Oligonucleotide Array Sequence Analysis; Stem Cells; Time Factors
PY - 2006
Y1 - 2006
N2 - Traumatic injury to the brain is one of the leading causes of injury-related death or disability, especially among young people. Inflammatory processes and oxidative stress likely underlie much of the damage elicited by injury, but the full repertoire of responses involved is not well known. A genomic approach, such as the use of microarrays, provides much insight in this regard, especially if combined with the use of gene-targeted animals. We report here the results of one of these studies comparing wild-type and metallothionein-I + II knockout mice subjected to a cryolesion of the somatosensorial cortex and killed at 0, 1, 4, 8, and 16 days postlesion (dpl) using Affymetrix genechips/oligonucleotide arrays interrogating approximately 10,000 different murine genes (MG_U74Av2). Hierarchical clustering analysis of these genes readily shows an orderly pattern of gene responses at specific times consistent with the processes involved in the initial tissue injury and later regeneration of the parenchyma, as well as a prominent effect of MT-I + II deficiency. The results thoroughly confirmed the importance of the antioxidant proteins MT-I + II in the response of the brain to injury and opened new avenues that were confirmed by immunohistochemistry. Data in KO, MT-I-overexpressing, and MT-II-injected mice strongly suggest a role of these proteins in postlesional activation of neural stem cells.
AB - Traumatic injury to the brain is one of the leading causes of injury-related death or disability, especially among young people. Inflammatory processes and oxidative stress likely underlie much of the damage elicited by injury, but the full repertoire of responses involved is not well known. A genomic approach, such as the use of microarrays, provides much insight in this regard, especially if combined with the use of gene-targeted animals. We report here the results of one of these studies comparing wild-type and metallothionein-I + II knockout mice subjected to a cryolesion of the somatosensorial cortex and killed at 0, 1, 4, 8, and 16 days postlesion (dpl) using Affymetrix genechips/oligonucleotide arrays interrogating approximately 10,000 different murine genes (MG_U74Av2). Hierarchical clustering analysis of these genes readily shows an orderly pattern of gene responses at specific times consistent with the processes involved in the initial tissue injury and later regeneration of the parenchyma, as well as a prominent effect of MT-I + II deficiency. The results thoroughly confirmed the importance of the antioxidant proteins MT-I + II in the response of the brain to injury and opened new avenues that were confirmed by immunohistochemistry. Data in KO, MT-I-overexpressing, and MT-II-injected mice strongly suggest a role of these proteins in postlesional activation of neural stem cells.
U2 - 10.1002/jnr.21043
DO - 10.1002/jnr.21043
M3 - Journal article
C2 - 16941634
SN - 0360-4012
VL - 84
SP - 1452
EP - 1474
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
IS - 7
ER -