TY - JOUR
T1 - Novel tethered particle motion analysis of CI protein-mediated DNA looping in the regulation of bacteriophage lambda
AU - Zurla, C
AU - Franzini, A
AU - Galli, Giorgio Giacomo
AU - Dunlap, DD
AU - Lewis, DEA
AU - Adhya, S
AU - Finzi, L
PY - 2006
Y1 - 2006
N2 - The tethered particle motion (TPM) technique has attracted great interest because of its simplicity and the wealth of information that it can provide on protein-induced conformational changes in nucleic acids. Here we present an approach to TPM methodology and analysis that increases the efficiency of data acquisition and facilitates interpretation of TPM assays. In particular, the statistical analysis that we propose allows fast data processing, minimal data selection and visual display of the distribution of molecular behaviour. The methodology proved useful in verifying CI protein-mediated DNA looping in bacteriophage ¿ and in differentiating between two different types of loops, stable and dynamic, whose relative occurrence seems to be a function of the distance between the operators as well as their relative angular orientation. Furthermore, the statistical analysis indicates that CI binding per se slightly shortens the DNA.
AB - The tethered particle motion (TPM) technique has attracted great interest because of its simplicity and the wealth of information that it can provide on protein-induced conformational changes in nucleic acids. Here we present an approach to TPM methodology and analysis that increases the efficiency of data acquisition and facilitates interpretation of TPM assays. In particular, the statistical analysis that we propose allows fast data processing, minimal data selection and visual display of the distribution of molecular behaviour. The methodology proved useful in verifying CI protein-mediated DNA looping in bacteriophage ¿ and in differentiating between two different types of loops, stable and dynamic, whose relative occurrence seems to be a function of the distance between the operators as well as their relative angular orientation. Furthermore, the statistical analysis indicates that CI binding per se slightly shortens the DNA.
M3 - Journal article
JO - Journal of Physics: Condensed Matter
JF - Journal of Physics: Condensed Matter
SN - 0953-8984
IS - 14
ER -