Nuclei size in relation to nuclear status and aneuploidy rate for 13 chromosomes in donated four cells embryos

I.E. Agerholm; C. Hnida; D.G. Cruger; C. Berg; G. Bruun-Petersen; S. Kolvraa; S. Ziebe

Nuclei size in relation to nuclear status and aneuploidy rate for 13 chromosomes in donated four cells embryos

I.E. Agerholm, C. Hnida, D.G. Cruger, C. Berg, G. Bruun-Petersen, S. Kolvraa, S. Ziebe

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

25 Citationer (Scopus)

Abstract

Purpose The aim was to elucidate if the nuclear size and number are indicative of aberrant chromosome content in human blastomeres and embryos. Methods The number of nuclei and the nucleus and blastomere size were measured by a computer controlled system for multilevel analysis. Then the nuclei were enumerated for 13 chromosomes by a combination of PNA and DNA probes. Results In the mononucleated embryos there was no difference in the mean size of chromosomally normal and abnormal nuclei but a significant difference in the mean nuclei size of nuclei that had gained chromosomes compared to nuclei that had lost chromosomes. The nuclei from multinucleated blastomeres had a significant smaller mean size and the frequency of chromosomally aberrant blastomeres was significantly higher. Conclusion The mean nuclear size is not a marker for the chromosome content in mononucleated embryos. However, it seems that the nuclei size can be related to multinucleation and maybe to the chromosome content
Udgivelsesdato: 2008/3

Originalsprog	Engelsk
Tidsskrift	Journal of Assisted Reproduction and Genetics
Vol/bind	25
Udgave nummer	2-3
Sider (fra-til)	95-102
Antal sider	7
ISSN	1058-0468
Status	Udgivet - 2008

Bibliografisk note

Times Cited: 1ArticleEnglishAgerholm, I. EBraedstrup Hosp, Fertil Clin, Sygehusvej 20, DK-8740 Braedstrup, DenmarkCited References Count: 23269WRSPRINGER/PLENUM PUBLISHERS233 SPRING ST, NEW YORK, NY 10013 USANEW YORK

Citationsformater

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title = "Nuclei size in relation to nuclear status and aneuploidy rate for 13 chromosomes in donated four cells embryos",

abstract = "Purpose The aim was to elucidate if the nuclear size and number are indicative of aberrant chromosome content in human blastomeres and embryos. Methods The number of nuclei and the nucleus and blastomere size were measured by a computer controlled system for multilevel analysis. Then the nuclei were enumerated for 13 chromosomes by a combination of PNA and DNA probes. Results In the mononucleated embryos there was no difference in the mean size of chromosomally normal and abnormal nuclei but a significant difference in the mean nuclei size of nuclei that had gained chromosomes compared to nuclei that had lost chromosomes. The nuclei from multinucleated blastomeres had a significant smaller mean size and the frequency of chromosomally aberrant blastomeres was significantly higher. Conclusion The mean nuclear size is not a marker for the chromosome content in mononucleated embryos. However, it seems that the nuclei size can be related to multinucleation and maybe to the chromosome content Udgivelsesdato: 2008/3",

author = "I.E. Agerholm and C. Hnida and D.G. Cruger and C. Berg and G. Bruun-Petersen and S. Kolvraa and S. Ziebe",

note = "Times Cited: 1ArticleEnglishAgerholm, I. EBraedstrup Hosp, Fertil Clin, Sygehusvej 20, DK-8740 Braedstrup, DenmarkCited References Count: 23269WRSPRINGER/PLENUM PUBLISHERS233 SPRING ST, NEW YORK, NY 10013 USANEW YORK",

year = "2008",

language = "English",

volume = "25",

pages = "95--102",

journal = "Journal of Assisted Reproduction and Genetics",

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TY - JOUR

T1 - Nuclei size in relation to nuclear status and aneuploidy rate for 13 chromosomes in donated four cells embryos

AU - Agerholm, I.E.

AU - Hnida, C.

AU - Cruger, D.G.

AU - Berg, C.

AU - Bruun-Petersen, G.

AU - Kolvraa, S.

AU - Ziebe, S.

N1 - Times Cited: 1ArticleEnglishAgerholm, I. EBraedstrup Hosp, Fertil Clin, Sygehusvej 20, DK-8740 Braedstrup, DenmarkCited References Count: 23269WRSPRINGER/PLENUM PUBLISHERS233 SPRING ST, NEW YORK, NY 10013 USANEW YORK

PY - 2008

Y1 - 2008

N2 - Purpose The aim was to elucidate if the nuclear size and number are indicative of aberrant chromosome content in human blastomeres and embryos. Methods The number of nuclei and the nucleus and blastomere size were measured by a computer controlled system for multilevel analysis. Then the nuclei were enumerated for 13 chromosomes by a combination of PNA and DNA probes. Results In the mononucleated embryos there was no difference in the mean size of chromosomally normal and abnormal nuclei but a significant difference in the mean nuclei size of nuclei that had gained chromosomes compared to nuclei that had lost chromosomes. The nuclei from multinucleated blastomeres had a significant smaller mean size and the frequency of chromosomally aberrant blastomeres was significantly higher. Conclusion The mean nuclear size is not a marker for the chromosome content in mononucleated embryos. However, it seems that the nuclei size can be related to multinucleation and maybe to the chromosome content Udgivelsesdato: 2008/3

AB - Purpose The aim was to elucidate if the nuclear size and number are indicative of aberrant chromosome content in human blastomeres and embryos. Methods The number of nuclei and the nucleus and blastomere size were measured by a computer controlled system for multilevel analysis. Then the nuclei were enumerated for 13 chromosomes by a combination of PNA and DNA probes. Results In the mononucleated embryos there was no difference in the mean size of chromosomally normal and abnormal nuclei but a significant difference in the mean nuclei size of nuclei that had gained chromosomes compared to nuclei that had lost chromosomes. The nuclei from multinucleated blastomeres had a significant smaller mean size and the frequency of chromosomally aberrant blastomeres was significantly higher. Conclusion The mean nuclear size is not a marker for the chromosome content in mononucleated embryos. However, it seems that the nuclei size can be related to multinucleation and maybe to the chromosome content Udgivelsesdato: 2008/3

M3 - Journal article

SN - 1058-0468

VL - 25

SP - 95

EP - 102

JO - Journal of Assisted Reproduction and Genetics

JF - Journal of Assisted Reproduction and Genetics

IS - 2-3

ER -