TY - JOUR
T1 - On the role of MT1-MMP, a matrix metalloproteinase essential to collagen remodeling, in murine molar eruption and root growth
AU - Beertsen, Wouter
AU - Holmbeck, Kenn
AU - Niehof, Anneke
AU - Bianco, Paolo
AU - Chrysovergis, Kaliiopi
AU - Birkedal-Hansen, Henning
AU - Everts, Vincent
PY - 2002/12
Y1 - 2002/12
N2 - Although the connective tissues of the periodontium are subject to a high turnover rate, no conclusive evidence has yet emerged that periodontal collagen turnover is essential for the eruption of teeth or for root elongation. These processes were studied in mice deficient in MT1-MMP, a membrane type matrix metalloproteinase essential for remodeling of soft tissue-hard tissue interfaces. Mandibular first molars of deficient mice and their wild-type littermates were subjected to stereological analysis in order to assess root length, eruption and the volume density of phagocytosed collagen in periodontal ligament fibroblasts. The data showed that both eruption and root elongation were severely inhibited in animals lacking the enzyme. We also found, in periodontal ligament fibroblasts from MT1-MMP-deficient mice, a massive age-related accumulation (up to 60-fold over controls) of collagen fibril-containing phagosomes. Phagolysosomes, which represent the next downstream step in collagen fibril degradation by the lysosomal pathway, did not accumulate. These observations indicate that MT1-MMP plays a central role in periodontal remodeling. The stunted root growth and the failure to erupt indicate the important role of the enzyme in tooth development.
AB - Although the connective tissues of the periodontium are subject to a high turnover rate, no conclusive evidence has yet emerged that periodontal collagen turnover is essential for the eruption of teeth or for root elongation. These processes were studied in mice deficient in MT1-MMP, a membrane type matrix metalloproteinase essential for remodeling of soft tissue-hard tissue interfaces. Mandibular first molars of deficient mice and their wild-type littermates were subjected to stereological analysis in order to assess root length, eruption and the volume density of phagocytosed collagen in periodontal ligament fibroblasts. The data showed that both eruption and root elongation were severely inhibited in animals lacking the enzyme. We also found, in periodontal ligament fibroblasts from MT1-MMP-deficient mice, a massive age-related accumulation (up to 60-fold over controls) of collagen fibril-containing phagosomes. Phagolysosomes, which represent the next downstream step in collagen fibril degradation by the lysosomal pathway, did not accumulate. These observations indicate that MT1-MMP plays a central role in periodontal remodeling. The stunted root growth and the failure to erupt indicate the important role of the enzyme in tooth development.
KW - Animals
KW - Fibrillar Collagens/metabolism
KW - Fibroblasts/enzymology
KW - Matrix Metalloproteinase 14
KW - Matrix Metalloproteinases, Membrane-Associated
KW - Metalloendopeptidases/physiology
KW - Mice
KW - Mice, Mutant Strains
KW - Molar/enzymology
KW - Periodontal Ligament/cytology
KW - Phagosomes/ultrastructure
KW - Tooth Eruption/physiology
KW - Tooth Root/enzymology
M3 - Journal article
C2 - 12507218
VL - 110
SP - 445
EP - 451
JO - European Journal of Oral Sciences
JF - European Journal of Oral Sciences
SN - 0909-8836
IS - 6
ER -