Oxidative Crosslinking of Peptides and Proteins: Mechanisms of Formation, Detection, Characterization and Quantification

Eduardo Fuentes-Lemus, Per Hägglund, Camilo López-Alarcón, Michael J. Davies*

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskriftReviewForskningpeer review

43 Citationer (Scopus)
38 Downloads (Pure)

Abstract

Covalent crosslinks within or between proteins play a key role in determining the structure and function of proteins. Some of these are formed intentionally by either enzymatic or molecular reactions and are critical to normal physiological function. Others are generated as a consequence of exposure to oxidants (radicals, excited states or two-electron species) and other endogenous or external stimuli, or as a result of the actions of a number of enzymes (e.g., oxidases and peroxidases). Increasing evidence indicates that the accumulation of unwanted crosslinks, as is seen in ageing and multiple pathologies, has adverse effects on biological function. In this article, we review the spectrum of crosslinks, both reducible and non-reducible, currently known to be formed on proteins; the mechanisms of their formation; and experimental approaches to the detection, identification and characterization of these species.

OriginalsprogEngelsk
Artikelnummer15
TidsskriftMolecules
Vol/bind27
Udgave nummer1
Sider (fra-til)1-31
ISSN1431-5157
DOI
StatusUdgivet - 1 jan. 2022

Bibliografisk note

Funding Information:
Funding: This project received funding from the European Union’s Horizon 2020 Research and Innovation Programme under the Marie Skłodowska-Curie grant agreement no. 890681 (to E.F.L.), the Novo Nordisk Foundation (NNF13OC0004294, NNF19OC0058493, and NNF20SA0064214 to M.J.D.), the Carlsberg Foundation (CF19-0451 to P.H.) and Fondecyt (grant no. 1180642 to C.L.A.).

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

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