pH-rate profiles of L-arabinitol 4-dehydrogenase from Hypocrea jecorina and its application in L-xylulose production

Manish Kumar Tiwari; Raushan Kumar Singh; Hui Gao; Taesu Kim; Suhwan Chang; Han S Kim; Jung-Kul Lee

doi:10.1016/j.bmcl.2013.11.047

pH-rate profiles of L-arabinitol 4-dehydrogenase from Hypocrea jecorina and its application in L-xylulose production

Manish Kumar Tiwari, Raushan Kumar Singh, Hui Gao, Taesu Kim, Suhwan Chang, Han S Kim, Jung-Kul Lee

Publikation: Bidrag til tidsskrift › Letter › peer review

15 Citationer (Scopus)

Abstract

l-Arabinitol 4-dehydrogenase (LAD) from Hypocrea jecorina (HjLAD) was cloned and overexpressed in Escherichia coli BL21 (DE3). The kinetics of l-arabinitol oxidation by NAD(+), catalyzed by HjLAD, was studied within the pH range of 7.0-9.5 at 25°C. The turnover number (kcat) and the catalytic efficiency (kcat/Km) were 4200min(-1) and 290mM(-1)min(-1), respectively. HjLAD showed the highest turnover number and catalytic efficiency among all previously characterized LADs. In further application of HjLAD, rare l-sugar l-xylulose was produced by the enzymatic oxidation of arabinitol to give a yield of approximately 86%.

Originalsprog	Engelsk
Tidsskrift	Bioorganic & Medicinal Chemistry Letters
Vol/bind	24
Udgave nummer	1
Sider (fra-til)	173-176
Antal sider	4
ISSN	0960-894X
DOI	https://doi.org/10.1016/j.bmcl.2013.11.047
Status	Udgivet - 2014
Udgivet eksternt	Ja

Adgang til dokumentet

10.1016/j.bmcl.2013.11.047

Citationsformater

@article{d6b678ddeca440a0acae1026cd9f2b28,

title = "pH-rate profiles of L-arabinitol 4-dehydrogenase from Hypocrea jecorina and its application in L-xylulose production",

abstract = "l-Arabinitol 4-dehydrogenase (LAD) from Hypocrea jecorina (HjLAD) was cloned and overexpressed in Escherichia coli BL21 (DE3). The kinetics of l-arabinitol oxidation by NAD(+), catalyzed by HjLAD, was studied within the pH range of 7.0-9.5 at 25°C. The turnover number (kcat) and the catalytic efficiency (kcat/Km) were 4200min(-1) and 290mM(-1)min(-1), respectively. HjLAD showed the highest turnover number and catalytic efficiency among all previously characterized LADs. In further application of HjLAD, rare l-sugar l-xylulose was produced by the enzymatic oxidation of arabinitol to give a yield of approximately 86%.",

keywords = "Biocatalysis, Hydrogen-Ion Concentration, Hypocrea, Kinetics, Models, Molecular, Recombinant Proteins, Sugar Alcohol Dehydrogenases, Xylulose",

author = "Tiwari, {Manish Kumar} and Singh, {Raushan Kumar} and Hui Gao and Taesu Kim and Suhwan Chang and Kim, {Han S} and Jung-Kul Lee",

year = "2014",

doi = "10.1016/j.bmcl.2013.11.047",

language = "English",

volume = "24",

pages = "173--176",

journal = "Bioorganic & Medicinal Chemistry Letters",

issn = "0960-894X",

publisher = "Pergamon Press",

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TY - JOUR

T1 - pH-rate profiles of L-arabinitol 4-dehydrogenase from Hypocrea jecorina and its application in L-xylulose production

AU - Tiwari, Manish Kumar

AU - Singh, Raushan Kumar

AU - Gao, Hui

AU - Kim, Taesu

AU - Chang, Suhwan

AU - Kim, Han S

AU - Lee, Jung-Kul

PY - 2014

Y1 - 2014

N2 - l-Arabinitol 4-dehydrogenase (LAD) from Hypocrea jecorina (HjLAD) was cloned and overexpressed in Escherichia coli BL21 (DE3). The kinetics of l-arabinitol oxidation by NAD(+), catalyzed by HjLAD, was studied within the pH range of 7.0-9.5 at 25°C. The turnover number (kcat) and the catalytic efficiency (kcat/Km) were 4200min(-1) and 290mM(-1)min(-1), respectively. HjLAD showed the highest turnover number and catalytic efficiency among all previously characterized LADs. In further application of HjLAD, rare l-sugar l-xylulose was produced by the enzymatic oxidation of arabinitol to give a yield of approximately 86%.

AB - l-Arabinitol 4-dehydrogenase (LAD) from Hypocrea jecorina (HjLAD) was cloned and overexpressed in Escherichia coli BL21 (DE3). The kinetics of l-arabinitol oxidation by NAD(+), catalyzed by HjLAD, was studied within the pH range of 7.0-9.5 at 25°C. The turnover number (kcat) and the catalytic efficiency (kcat/Km) were 4200min(-1) and 290mM(-1)min(-1), respectively. HjLAD showed the highest turnover number and catalytic efficiency among all previously characterized LADs. In further application of HjLAD, rare l-sugar l-xylulose was produced by the enzymatic oxidation of arabinitol to give a yield of approximately 86%.

KW - Biocatalysis

KW - Hydrogen-Ion Concentration

KW - Hypocrea

KW - Kinetics

KW - Models, Molecular

KW - Recombinant Proteins

KW - Sugar Alcohol Dehydrogenases

KW - Xylulose

U2 - 10.1016/j.bmcl.2013.11.047

DO - 10.1016/j.bmcl.2013.11.047

M3 - Letter

C2 - 24342239

SN - 0960-894X

VL - 24

SP - 173

EP - 176

JO - Bioorganic & Medicinal Chemistry Letters

JF - Bioorganic & Medicinal Chemistry Letters

IS - 1

ER -