Abstract
Originalsprog | Engelsk |
---|---|
Tidsskrift | Bioconjugate Chemistry |
Vol/bind | 2 |
Sider (fra-til) | 57-66 |
ISSN | 1043-1802 |
DOI | |
Status | Udgivet - 1991 |
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Photolytic Cleavage of DNA by Nitrobenzamido Ligands Linked to 9-Aminoacridines Gives DNA Polymerase Substrates in a Wavelength-Dependent Reaction. / Nielsen, Peter E.; Egholm, M.; Koch, T.; Christensen, Jørn Bolstad; Buchardt, Ole.
I: Bioconjugate Chemistry, Bind 2, 1991, s. 57-66.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Photolytic Cleavage of DNA by Nitrobenzamido Ligands Linked to 9-Aminoacridines Gives DNA Polymerase Substrates in a Wavelength-Dependent Reaction
AU - Nielsen, Peter E.
AU - Egholm, M.
AU - Koch, T.
AU - Christensen, Jørn Bolstad
AU - Buchardt, Ole
PY - 1991
Y1 - 1991
N2 - A series of reagents containing 3- or 4-nitrobenzamido ligands tethered to 9-aminoacridine via variable-length linkers have been prepared and their properties as photochemical DNA cleavers (photonucleases) examined. When irradiated with approximately 300-nm light, where the nitrobenzamido ligand can absorb, they cleave DNA in an oxygen-independent reaction presumably involving oxygen transfer from the nitro group to the deoxyribose units of the DNA backbone (Nielsen et al., 1988b). This reaction is pH independent and only slightly affected by the linker length, and the DNA fragments are not substrates for DNA polymerase. When approximately 420-nm light is used, were only the 9-aminoacridinyl ligands absorb, the DNA cleavage is also oxygen-independent but pH dependent, requires DNA saturation with the reagent (base pair:reagent less than or equal to 2), and is most efficient with the longer linkers. The cleavage is specific for guanine residues and results in 5'-phosphate termini and heterogeneous (more than four products) 3'-termini. One of the products is presumably 3'-hydroxy since DNA photocleaved with nitrobenzamido acridine reagents and 420-nm radiation are substrates for DNA polymerase in a nick translation assay as well as for the Klenow fragment. An electron-transfer mechanism is suggested.
AB - A series of reagents containing 3- or 4-nitrobenzamido ligands tethered to 9-aminoacridine via variable-length linkers have been prepared and their properties as photochemical DNA cleavers (photonucleases) examined. When irradiated with approximately 300-nm light, where the nitrobenzamido ligand can absorb, they cleave DNA in an oxygen-independent reaction presumably involving oxygen transfer from the nitro group to the deoxyribose units of the DNA backbone (Nielsen et al., 1988b). This reaction is pH independent and only slightly affected by the linker length, and the DNA fragments are not substrates for DNA polymerase. When approximately 420-nm light is used, were only the 9-aminoacridinyl ligands absorb, the DNA cleavage is also oxygen-independent but pH dependent, requires DNA saturation with the reagent (base pair:reagent less than or equal to 2), and is most efficient with the longer linkers. The cleavage is specific for guanine residues and results in 5'-phosphate termini and heterogeneous (more than four products) 3'-termini. One of the products is presumably 3'-hydroxy since DNA photocleaved with nitrobenzamido acridine reagents and 420-nm radiation are substrates for DNA polymerase in a nick translation assay as well as for the Klenow fragment. An electron-transfer mechanism is suggested.
U2 - 10.1021/bc00007a010
DO - 10.1021/bc00007a010
M3 - Journal article
VL - 2
SP - 57
EP - 66
JO - Bioconjugate Chemistry
JF - Bioconjugate Chemistry
SN - 1043-1802
ER -