Pneumocystis carinii in bronchoalveolar lavage and induced sputum: detection with a nested polymerase chain reaction.

Bidragets oversatte titel: Pneumocystis carinii in bronchoalveolar lavage and induced sputum: detection with a nested polymerase chain reaction.

J Skøt, A G Lerche, H J Kolmos, Jens Ole Nielsen, Lars Reinhardt Mathiesen, Jens Dilling Lundgren

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20 Citationer (Scopus)

Abstract

To evaluate polymerase chain reaction (PCR) for detection of Pneumocystis carinii, 117 bronchoalveolar lavage (BAL) specimens, from HIV-infected patients undergoing a diagnostic bronchoscopy, were processed and a nested PCR, followed by Southern blot and hybridization with a P32-labelled probe was performed. The sensitivity and specificity were 85 and 100% 934/40 and 77/77) respectively. A non-radioactive labelling system BluGENE was evaluated on all specimens, and found to be as effective as P32-labelling. To increase the speed and convenience of detection, a dot blot system was tested, but sensitivity dropped markedly with this system. A further 33 patients had both induced sputum and bronchoalveolar lavage performed and the induced sputum was analysed using PCR and routine microbiological methods. The PCR sensitivity on induced sputum was equal to that of routine methods. At present the evaluated PCR cannot replace routine microbiological methods for detection of Pneumocystis carinii, on either BAL fluid or induced sputum.
Bidragets oversatte titelPneumocystis carinii in bronchoalveolar lavage and induced sputum: detection with a nested polymerase chain reaction.
OriginalsprogEngelsk
TidsskriftScandinavian Journal of Infectious Diseases
Vol/bind27
Udgave nummer4
Sider (fra-til)363-367
Antal sider5
ISSN0036-5548
StatusUdgivet - 1995

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