Possible involvement of GABA(A) and GABA(B) receptors in the inhibitory action of lindane on transmitter release from cerebellar granule neurons.

Inge Damgaard; G Nyitrai; I Kovács; J Kardos; A Schousboe

Possible involvement of GABA(A) and GABA(B) receptors in the inhibitory action of lindane on transmitter release from cerebellar granule neurons.

Inge Damgaard, G Nyitrai, I Kovács, J Kardos, A Schousboe

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

4 Citationer (Scopus)

Abstract

Udgivelsesdato: 1999-Sep

Originalsprog	Engelsk
Tidsskrift	Neurochemical Research
Vol/bind	24
Udgave nummer	9
Sider (fra-til)	1189-93
Antal sider	4
ISSN	0364-3190
Status	Udgivet - 1999
Udgivet eksternt	Ja

Bibliografisk note

Keywords: Animals; Aspartic Acid; Baclofen; Cells, Cultured; Cerebellum; Cytoplasmic Granules; Lindane; Mice; Neurons; Radioligand Assay; Receptors, GABA-A; Receptors, GABA-B

Citationsformater

@article{a044f78058a711dd8d9f000ea68e967b,

title = "Possible involvement of GABA(A) and GABA(B) receptors in the inhibitory action of lindane on transmitter release from cerebellar granule neurons.",

abstract = "Cerebellar granule cells in culture express receptors for GABA belonging to the GABA(A) and GABA(B) classes. In order to characterize the ability of the insecticide lindane to interact with these receptors cells were grown in either plain culture media or media containing 150 microM THIP as this is known to influence the properties of both GABA(A) and GABA(B) receptors. It was found that lindane regardless of the culture condition inhibited evoked (40 mM K+) release of neurotransmitter ([3H]D-aspartate as label for glutamate). In naive cells both GABA(A) and GABA(B) receptor active drugs prevented the inhibitory action of lindane but in THIP treated cultures none of the GABA(A) and GABA(B) receptor active drugs had any effect on the inhibitory action of lindane. This lack of effect was not due to inability of baclofen itself to inhibit transmitter release. It is concluded that lindane dependent on the state of the GABA(A) and GABA(B) receptors is able to indirectly interfere with both GABA(A) and GABA(B) receptors. In case of the latter receptors it was shown using [3H]baclofen to label the receptors that lindane could not displace the ligand confirming that lindane is likely to exert its action at a site different from the agonist binding site.",

author = "Inge Damgaard and G Nyitrai and I Kov{\'a}cs and J Kardos and A Schousboe",

note = "Keywords: Animals; Aspartic Acid; Baclofen; Cells, Cultured; Cerebellum; Cytoplasmic Granules; Lindane; Mice; Neurons; Radioligand Assay; Receptors, GABA-A; Receptors, GABA-B",

year = "1999",

language = "English",

volume = "24",

pages = "1189--93",

journal = "Neurochemical Research",

issn = "0364-3190",

publisher = "Springer",

number = "9",

}

TY - JOUR

T1 - Possible involvement of GABA(A) and GABA(B) receptors in the inhibitory action of lindane on transmitter release from cerebellar granule neurons.

AU - Damgaard, Inge

AU - Nyitrai, G

AU - Kovács, I

AU - Kardos, J

AU - Schousboe, A

N1 - Keywords: Animals; Aspartic Acid; Baclofen; Cells, Cultured; Cerebellum; Cytoplasmic Granules; Lindane; Mice; Neurons; Radioligand Assay; Receptors, GABA-A; Receptors, GABA-B

PY - 1999

Y1 - 1999

N2 - Cerebellar granule cells in culture express receptors for GABA belonging to the GABA(A) and GABA(B) classes. In order to characterize the ability of the insecticide lindane to interact with these receptors cells were grown in either plain culture media or media containing 150 microM THIP as this is known to influence the properties of both GABA(A) and GABA(B) receptors. It was found that lindane regardless of the culture condition inhibited evoked (40 mM K+) release of neurotransmitter ([3H]D-aspartate as label for glutamate). In naive cells both GABA(A) and GABA(B) receptor active drugs prevented the inhibitory action of lindane but in THIP treated cultures none of the GABA(A) and GABA(B) receptor active drugs had any effect on the inhibitory action of lindane. This lack of effect was not due to inability of baclofen itself to inhibit transmitter release. It is concluded that lindane dependent on the state of the GABA(A) and GABA(B) receptors is able to indirectly interfere with both GABA(A) and GABA(B) receptors. In case of the latter receptors it was shown using [3H]baclofen to label the receptors that lindane could not displace the ligand confirming that lindane is likely to exert its action at a site different from the agonist binding site.

AB - Cerebellar granule cells in culture express receptors for GABA belonging to the GABA(A) and GABA(B) classes. In order to characterize the ability of the insecticide lindane to interact with these receptors cells were grown in either plain culture media or media containing 150 microM THIP as this is known to influence the properties of both GABA(A) and GABA(B) receptors. It was found that lindane regardless of the culture condition inhibited evoked (40 mM K+) release of neurotransmitter ([3H]D-aspartate as label for glutamate). In naive cells both GABA(A) and GABA(B) receptor active drugs prevented the inhibitory action of lindane but in THIP treated cultures none of the GABA(A) and GABA(B) receptor active drugs had any effect on the inhibitory action of lindane. This lack of effect was not due to inability of baclofen itself to inhibit transmitter release. It is concluded that lindane dependent on the state of the GABA(A) and GABA(B) receptors is able to indirectly interfere with both GABA(A) and GABA(B) receptors. In case of the latter receptors it was shown using [3H]baclofen to label the receptors that lindane could not displace the ligand confirming that lindane is likely to exert its action at a site different from the agonist binding site.

M3 - Journal article

C2 - 10485591

SN - 0364-3190

VL - 24

SP - 1189

EP - 1193

JO - Neurochemical Research

JF - Neurochemical Research

IS - 9

ER -