Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Journal of Vascular Research |
| Vol/bind | 44 |
| Udgave nummer | 4 |
| Sider (fra-til) | 253-63 |
| Antal sider | 10 |
| ISSN | 1018-1172 |
| DOI | |
| Status | Udgivet - 2007 |
| Udgivet eksternt | Ja |
Bibliografisk note
Copyright (c) 2007 S. Karger AG, Basel.Citationsformater
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I: Journal of Vascular Research, Bind 44, Nr. 4, 2007, s. 253-63.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Production of inflammatory molecules in peripheral blood mononuclear cells from severely glucose-6-phosphate dehydrogenase-deficient subjects.
AU - Sanna, Francesca
AU - Bonatesta, Rosa Rita
AU - Frongia, Bruno
AU - Uda, Sabrina
AU - Banni, Sebastiano
AU - Melis, Maria Paola
AU - Collu, Maria
AU - Madeddu, Clelia
AU - Serpe, Roberto
AU - Puddu, Silvana
AU - Porcu, Giovanna
AU - Dessì, Sandra
AU - Batetta, Barbara
N1 - Keywords: Adult; Biological Markers; Cells, Cultured; Cholesterol; Cytokines; Esterification; Fatty Acids, Unsaturated; Foam Cells; Glucosephosphate Dehydrogenase; Glucosephosphate Dehydrogenase Deficiency; Humans; Hydroxyeicosatetraenoic Acids; Leukocytes, Mononuclear; Macrophages; Male; Monocytes; Severity of Illness Index; Thymidine; Tritium
PY - 2007
Y1 - 2007
N2 - OBJECTIVE: We have previously demonstrated that Mediterranean glucose-6-phosphate dehydrogenase (G6PD)-deficient peripheral blood mononuclear cells (PBMC) respond to mitogenic stimuli with a reduced cholesterol synthesis and growth. In the present study, we have investigated the release of inflammatory molecules by PBMC following a mitogenic stimulus, as well as the transformation to foam cells of monocyte-derived macrophages from severely G6PD-deficient and normal subjects. METHODS AND RESULTS: PBMC from G6PD-deficient subjects produced interleukin (IL)-1beta and IL-6 to a lower extent compared with normal subjects. 5-Hydroxyeicosatetraenoic acid, a primary product of 5-lipoxygenase, was slightly decreased. Tumour necrosis factor-alpha and IL-1beta secretion was significantly reduced in monocyte-derived macrophages. No difference was found in IL-10 secretion, whereas transforming growth factor-beta was invariably found to be significantly higher in G6PD-deficient cells. In cells incubated with acetylated low-density lipoprotein, cholesterol esterification and its storage in lipid droplets were lower than in normal G6PD cells. CONCLUSIONS: We conclude that by reducing the secretion of inflammatory molecules by PBMC and increasing the secretion of transforming growth factor-beta and the capability of monocyte-derived macrophages to accumulate lipid droplets and convert into foam cells, G6PD deficiency may confer a partial protection against atherosclerosis leading to the reduced risk of cardiovascular diseases reported in G6PD-deficient subjects.
AB - OBJECTIVE: We have previously demonstrated that Mediterranean glucose-6-phosphate dehydrogenase (G6PD)-deficient peripheral blood mononuclear cells (PBMC) respond to mitogenic stimuli with a reduced cholesterol synthesis and growth. In the present study, we have investigated the release of inflammatory molecules by PBMC following a mitogenic stimulus, as well as the transformation to foam cells of monocyte-derived macrophages from severely G6PD-deficient and normal subjects. METHODS AND RESULTS: PBMC from G6PD-deficient subjects produced interleukin (IL)-1beta and IL-6 to a lower extent compared with normal subjects. 5-Hydroxyeicosatetraenoic acid, a primary product of 5-lipoxygenase, was slightly decreased. Tumour necrosis factor-alpha and IL-1beta secretion was significantly reduced in monocyte-derived macrophages. No difference was found in IL-10 secretion, whereas transforming growth factor-beta was invariably found to be significantly higher in G6PD-deficient cells. In cells incubated with acetylated low-density lipoprotein, cholesterol esterification and its storage in lipid droplets were lower than in normal G6PD cells. CONCLUSIONS: We conclude that by reducing the secretion of inflammatory molecules by PBMC and increasing the secretion of transforming growth factor-beta and the capability of monocyte-derived macrophages to accumulate lipid droplets and convert into foam cells, G6PD deficiency may confer a partial protection against atherosclerosis leading to the reduced risk of cardiovascular diseases reported in G6PD-deficient subjects.
U2 - 10.1159/000100903
DO - 10.1159/000100903
M3 - Journal article
C2 - 17361089
SN - 1018-1172
VL - 44
SP - 253
EP - 263
JO - Journal of Vascular Research
JF - Journal of Vascular Research
IS - 4
ER -