Prolonged drug-induced hypothermia in experimental stroke.

Flemming Fryd Johansen; Henrik Stig Jørgensen; Jakob Reith

doi:10.1053/jscd.2003.14

Prolonged drug-induced hypothermia in experimental stroke.

Flemming Fryd Johansen, Henrik Stig Jørgensen, Jakob Reith

Biomedicinsk Institut

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

14 Citationer (Scopus)

Abstract

Udgivelsesdato: null-null

Originalsprog	Engelsk
Tidsskrift	Journal of Stroke & Cerebrovascular Diseases
Vol/bind	12
Udgave nummer	2
Sider (fra-til)	97-102
Antal sider	5
ISSN	1052-3057
DOI	https://doi.org/10.1053/jscd.2003.14
Status	Udgivet - 2007

Adgang til dokumentet

10.1053/jscd.2003.14

Citationsformater

@article{9540d9f0abf711ddb5e9000ea68e967b,

title = "Prolonged drug-induced hypothermia in experimental stroke.",

abstract = "In experimental and human stroke, hypothermia is strongly related to a favorable outcome. Previous attempts to manipulate the core temperature in focal cerebral ischemia have been based on mechanical cooling. The purpose of the study is to establish a model for long-term drug-induced hypothermia in focal ischemia by pharmacological alteration of the central thermoregulatory set-point. We tested the hypothesis that the dopaminergic agonist Talipexole, which induces hypothermia, reduces infarct size. Body temperature was monitored by a radio-pill-implant. Rats had reversible occlusion of the middle cerebral artery (MCAO) for 30 minutes. Thirty minutes after reflow, the experimental group of rats (n = 10) received an intravenous bolus injection of Talipexole followed by a continuous infusion of Talipexole during the following 24 hours. The control group of rats (n = 10) received a similar treatment regimen with saline only. All rats were killed 7 days after MCAO. Infarct volume was quantified stereologically. The mean body temperature (35.6 + 1.0 degrees C) during 24 hours after bolus injection of Talipexole was significantly lower than in control rats (37.3 +/- 0.5 degrees C), P < .05. Infarct volumes were significantly lower in the Talipexole group (4.7 +/- 1.9 mm3) than in the control group (8.8 +/- 4.7 mm3), P < .04. In the Talipexole treated rats we also observed a significant hypokalemia (P = .001) and a significantly lower index of relative degree of movement (P < .02). Our study shows that the core body temperature was reduced by 1.7 degrees C for 24 hours after MCAO in rats treated with Talipexole. This treatment induced a significant reduction of infarct volume at 7 days after focal ischemia by 47%. We suggest that the reduction in infarct volume is related to drug-induced hypothermia. The hypokalemia in the hypothermic rats is possibly explained by the observed lower degree of movement.",

author = "Johansen, {Flemming Fryd} and J{\o}rgensen, {Henrik Stig} and Jakob Reith",

year = "2007",

doi = "10.1053/jscd.2003.14",

language = "English",

volume = "12",

pages = "97--102",

journal = "Journal of Stroke & Cerebrovascular Diseases",

issn = "1052-3057",

publisher = "W.B.Saunders Co.",

number = "2",

}

TY - JOUR

T1 - Prolonged drug-induced hypothermia in experimental stroke.

AU - Johansen, Flemming Fryd

AU - Jørgensen, Henrik Stig

AU - Reith, Jakob

PY - 2007

Y1 - 2007

N2 - In experimental and human stroke, hypothermia is strongly related to a favorable outcome. Previous attempts to manipulate the core temperature in focal cerebral ischemia have been based on mechanical cooling. The purpose of the study is to establish a model for long-term drug-induced hypothermia in focal ischemia by pharmacological alteration of the central thermoregulatory set-point. We tested the hypothesis that the dopaminergic agonist Talipexole, which induces hypothermia, reduces infarct size. Body temperature was monitored by a radio-pill-implant. Rats had reversible occlusion of the middle cerebral artery (MCAO) for 30 minutes. Thirty minutes after reflow, the experimental group of rats (n = 10) received an intravenous bolus injection of Talipexole followed by a continuous infusion of Talipexole during the following 24 hours. The control group of rats (n = 10) received a similar treatment regimen with saline only. All rats were killed 7 days after MCAO. Infarct volume was quantified stereologically. The mean body temperature (35.6 + 1.0 degrees C) during 24 hours after bolus injection of Talipexole was significantly lower than in control rats (37.3 +/- 0.5 degrees C), P < .05. Infarct volumes were significantly lower in the Talipexole group (4.7 +/- 1.9 mm3) than in the control group (8.8 +/- 4.7 mm3), P < .04. In the Talipexole treated rats we also observed a significant hypokalemia (P = .001) and a significantly lower index of relative degree of movement (P < .02). Our study shows that the core body temperature was reduced by 1.7 degrees C for 24 hours after MCAO in rats treated with Talipexole. This treatment induced a significant reduction of infarct volume at 7 days after focal ischemia by 47%. We suggest that the reduction in infarct volume is related to drug-induced hypothermia. The hypokalemia in the hypothermic rats is possibly explained by the observed lower degree of movement.

AB - In experimental and human stroke, hypothermia is strongly related to a favorable outcome. Previous attempts to manipulate the core temperature in focal cerebral ischemia have been based on mechanical cooling. The purpose of the study is to establish a model for long-term drug-induced hypothermia in focal ischemia by pharmacological alteration of the central thermoregulatory set-point. We tested the hypothesis that the dopaminergic agonist Talipexole, which induces hypothermia, reduces infarct size. Body temperature was monitored by a radio-pill-implant. Rats had reversible occlusion of the middle cerebral artery (MCAO) for 30 minutes. Thirty minutes after reflow, the experimental group of rats (n = 10) received an intravenous bolus injection of Talipexole followed by a continuous infusion of Talipexole during the following 24 hours. The control group of rats (n = 10) received a similar treatment regimen with saline only. All rats were killed 7 days after MCAO. Infarct volume was quantified stereologically. The mean body temperature (35.6 + 1.0 degrees C) during 24 hours after bolus injection of Talipexole was significantly lower than in control rats (37.3 +/- 0.5 degrees C), P < .05. Infarct volumes were significantly lower in the Talipexole group (4.7 +/- 1.9 mm3) than in the control group (8.8 +/- 4.7 mm3), P < .04. In the Talipexole treated rats we also observed a significant hypokalemia (P = .001) and a significantly lower index of relative degree of movement (P < .02). Our study shows that the core body temperature was reduced by 1.7 degrees C for 24 hours after MCAO in rats treated with Talipexole. This treatment induced a significant reduction of infarct volume at 7 days after focal ischemia by 47%. We suggest that the reduction in infarct volume is related to drug-induced hypothermia. The hypokalemia in the hypothermic rats is possibly explained by the observed lower degree of movement.

U2 - 10.1053/jscd.2003.14

DO - 10.1053/jscd.2003.14

M3 - Journal article

C2 - 17903912

VL - 12

SP - 97

EP - 102

JO - Journal of Stroke & Cerebrovascular Diseases

JF - Journal of Stroke & Cerebrovascular Diseases

SN - 1052-3057

IS - 2

ER -