Prolonged hypoxic culture and trypsinization increase the pro-angiogenic potential of human adipose tissue-derived stem cells

Jeppe Grøndahl Rasmussen; Ole Frøbert; Linda Pilgaard; Jens Kastrup; Ulf Simonsen; Vladimir Zachar; Trine Fink

doi:10.3109/14653249.2010.506505

Prolonged hypoxic culture and trypsinization increase the pro-angiogenic potential of human adipose tissue-derived stem cells

Jeppe Grøndahl Rasmussen, Ole Frøbert, Linda Pilgaard, Jens Kastrup, Ulf Simonsen, Vladimir Zachar, Trine Fink

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

89 Citationer (Scopus)

Abstract

Transplantation of mesenchymal stromal cells (MSC), including adipose tissue-derived stem cells (ASC), is a promising option in the treatment of vascular disease. Short-term hypoxic culture of MSC augments secretion of anti-apoptotic and angiogenic cytokines. We hypothesized that prolonged hypoxic (1% and 5% oxygen) culture and trypsinization would augment ASC expression of anti-apoptotic and angiogenic cytokines and increase the angiogenic potential of ASC-conditioned media.

Originalsprog	Engelsk
Tidsskrift	Cytotherapy
Vol/bind	13
Udgave nummer	3
Sider (fra-til)	318-28
Antal sider	11
ISSN	1465-3249
DOI	https://doi.org/10.3109/14653249.2010.506505
Status	Udgivet - 2011

Adgang til dokumentet

10.3109/14653249.2010.506505

Citationsformater

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title = "Prolonged hypoxic culture and trypsinization increase the pro-angiogenic potential of human adipose tissue-derived stem cells",

abstract = "Transplantation of mesenchymal stromal cells (MSC), including adipose tissue-derived stem cells (ASC), is a promising option in the treatment of vascular disease. Short-term hypoxic culture of MSC augments secretion of anti-apoptotic and angiogenic cytokines. We hypothesized that prolonged hypoxic (1% and 5% oxygen) culture and trypsinization would augment ASC expression of anti-apoptotic and angiogenic cytokines and increase the angiogenic potential of ASC-conditioned media.",

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TY - JOUR

T1 - Prolonged hypoxic culture and trypsinization increase the pro-angiogenic potential of human adipose tissue-derived stem cells

AU - Rasmussen, Jeppe Grøndahl

AU - Frøbert, Ole

AU - Pilgaard, Linda

AU - Kastrup, Jens

AU - Simonsen, Ulf

AU - Zachar, Vladimir

AU - Fink, Trine

PY - 2011

Y1 - 2011

N2 - Transplantation of mesenchymal stromal cells (MSC), including adipose tissue-derived stem cells (ASC), is a promising option in the treatment of vascular disease. Short-term hypoxic culture of MSC augments secretion of anti-apoptotic and angiogenic cytokines. We hypothesized that prolonged hypoxic (1% and 5% oxygen) culture and trypsinization would augment ASC expression of anti-apoptotic and angiogenic cytokines and increase the angiogenic potential of ASC-conditioned media.

AB - Transplantation of mesenchymal stromal cells (MSC), including adipose tissue-derived stem cells (ASC), is a promising option in the treatment of vascular disease. Short-term hypoxic culture of MSC augments secretion of anti-apoptotic and angiogenic cytokines. We hypothesized that prolonged hypoxic (1% and 5% oxygen) culture and trypsinization would augment ASC expression of anti-apoptotic and angiogenic cytokines and increase the angiogenic potential of ASC-conditioned media.

U2 - 10.3109/14653249.2010.506505

DO - 10.3109/14653249.2010.506505

M3 - Journal article

SN - 1465-3249

VL - 13

SP - 318

EP - 328

JO - Cytotherapy

JF - Cytotherapy

IS - 3

ER -