TY - JOUR
T1 - Protein aggregation capture on microparticles enables multi-purpose proteomics sample preparation
AU - Batth, Tanveer Singh
AU - Tollenaere, Maxim A. X.
AU - Rüther, Patrick Leopold
AU - Gonzalez-Franquesa, Alba
AU - Prabhakar, Bhargav Saligram
AU - Bekker-Jensen, Simon Holst
AU - Deshmukh, Atul Shahaji
AU - Olsen, Jesper V.
PY - 2019
Y1 - 2019
N2 - Universal proteomics sample preparation is challenging due to the high heterogeneity of biological samples. Here we describe a novel mechanism that exploits the inherent instability of denatured proteins for non-specific immobilization on microparticles by protein aggregation capture. To demonstrate the general applicability of this mechanism, we analyzed phosphoproteomes, tissue proteomes, and interaction proteomes as well as dilute secretomes. The findings presents a practical, sensitive and cost-effective proteomics sample preparation method.
AB - Universal proteomics sample preparation is challenging due to the high heterogeneity of biological samples. Here we describe a novel mechanism that exploits the inherent instability of denatured proteins for non-specific immobilization on microparticles by protein aggregation capture. To demonstrate the general applicability of this mechanism, we analyzed phosphoproteomes, tissue proteomes, and interaction proteomes as well as dilute secretomes. The findings presents a practical, sensitive and cost-effective proteomics sample preparation method.
U2 - 10.1074/mcp.TIR118.001270
DO - 10.1074/mcp.TIR118.001270
M3 - Journal article
C2 - 30833379
VL - 18
SP - 1027
EP - 1035
JO - Molecular and Cellular Proteomics
JF - Molecular and Cellular Proteomics
SN - 1535-9476
IS - 5
ER -