TY - JOUR
T1 - Proteomics-Based Comparative Mapping of the Secretomes of Human Brown and White Adipocytes Reveals EPDR1 as a Novel Batokine
AU - Deshmukh, Atul S
AU - Peijs, Lone
AU - Beaudry, Jacqueline L
AU - Jespersen, Naja Z
AU - Nielsen, Carsten H
AU - Ma, Tao
AU - Brunner, Andreas D
AU - Larsen, Therese J.
AU - Bayarri-Olmos, Rafael
AU - Prabhakar, Bhargav S
AU - Helgstrand, Charlotte
AU - Severinsen, Mai C K
AU - Holst, Birgitte
AU - Kjaer, Andreas
AU - Tang-Christensen, Mads
AU - Sanfridson, Annika
AU - Garred, Peter
AU - Privé, Gilbert G
AU - Pedersen, Bente K
AU - Gerhart-Hines, Zachary
AU - Nielsen, Søren
AU - Drucker, Daniel J
AU - Mann, Matthias
AU - Scheele, Camilla
PY - 2019
Y1 - 2019
N2 - Adipokines secreted from white adipose tissue play a role in metabolic crosstalk and homeostasis, whereas the brown adipose secretome is less explored. We performed high-sensitivity mass-spectrometry-based proteomics on the cell media of human adipocytes derived from the supraclavicular brown adipose and from the subcutaneous white adipose depots of adult humans. We identified 471 potentially secreted proteins covering interesting categories such as hormones, growth factors, extracellular matrix proteins, and proteins of the complement system, which were differentially regulated between brown and white adipocytes. A total of 101 proteins were exclusively quantified in brown adipocytes, and among these was ependymin-related protein 1 (EPDR1). EPDR1 was detected in human plasma, and functional studies suggested a role for EPDR1 in thermogenic determination during adipogenesis. In conclusion, we report substantial differences between the secretomes of brown and white human adipocytes and identify novel candidate batokines that can be important regulators of human metabolism.
AB - Adipokines secreted from white adipose tissue play a role in metabolic crosstalk and homeostasis, whereas the brown adipose secretome is less explored. We performed high-sensitivity mass-spectrometry-based proteomics on the cell media of human adipocytes derived from the supraclavicular brown adipose and from the subcutaneous white adipose depots of adult humans. We identified 471 potentially secreted proteins covering interesting categories such as hormones, growth factors, extracellular matrix proteins, and proteins of the complement system, which were differentially regulated between brown and white adipocytes. A total of 101 proteins were exclusively quantified in brown adipocytes, and among these was ependymin-related protein 1 (EPDR1). EPDR1 was detected in human plasma, and functional studies suggested a role for EPDR1 in thermogenic determination during adipogenesis. In conclusion, we report substantial differences between the secretomes of brown and white human adipocytes and identify novel candidate batokines that can be important regulators of human metabolism.
U2 - 10.1016/j.cmet.2019.10.001
DO - 10.1016/j.cmet.2019.10.001
M3 - Journal article
C2 - 31668873
VL - 30
SP - 963-975.e7
JO - Cell Metabolism
JF - Cell Metabolism
SN - 1550-4131
IS - 5
ER -