Proteomics strategy for quantitative protein interaction profiling in cell extracts

Kirti Sharma; Christoph Weber; Michaela Bairlein; Zoltán Greff; György Kéri; Jürgen Cox; Jesper V Olsen; Henrik Daub

doi:10.1038/nmeth.1373

Proteomics strategy for quantitative protein interaction profiling in cell extracts

Kirti Sharma, Christoph Weber, Michaela Bairlein, Zoltán Greff, György Kéri, Jürgen Cox, Jesper V Olsen, Henrik Daub

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

126 Citationer (Scopus)

Abstract

Udgivelsesdato: 2009-Oct

Originalsprog	Engelsk
Tidsskrift	Nature Methods
Vol/bind	6
Udgave nummer	10
Sider (fra-til)	741-4
Antal sider	3
ISSN	1548-7091
DOI	https://doi.org/10.1038/nmeth.1373
Status	Udgivet - 2009
Udgivet eksternt	Ja

Bibliografisk note

Keywords: Cell Extracts; Chromatography, Affinity; Mass Spectrometry; Protein Interaction Mapping; Proteome; Proteomics

Adgang til dokumentet

10.1038/nmeth.1373

Citationsformater

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title = "Proteomics strategy for quantitative protein interaction profiling in cell extracts",

abstract = "We report a proteomics strategy to both identify and quantify cellular target protein interactions with externally introduced ligands. We determined dissociation constants for target proteins interacting with the ligand of interest by combining quantitative mass spectrometry with a defined set of affinity purification experiments. We demonstrate the general utility of this methodology in interaction studies involving small-molecule kinase inhibitors, a tyrosine-phosphorylated peptide and an antibody as affinity ligands.",

author = "Kirti Sharma and Christoph Weber and Michaela Bairlein and Zolt{\'a}n Greff and Gy{\"o}rgy K{\'e}ri and J{\"u}rgen Cox and Olsen, {Jesper V} and Henrik Daub",

note = "Keywords: Cell Extracts; Chromatography, Affinity; Mass Spectrometry; Protein Interaction Mapping; Proteome; Proteomics",

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T1 - Proteomics strategy for quantitative protein interaction profiling in cell extracts

AU - Sharma, Kirti

AU - Weber, Christoph

AU - Bairlein, Michaela

AU - Greff, Zoltán

AU - Kéri, György

AU - Cox, Jürgen

AU - Olsen, Jesper V

AU - Daub, Henrik

N1 - Keywords: Cell Extracts; Chromatography, Affinity; Mass Spectrometry; Protein Interaction Mapping; Proteome; Proteomics

PY - 2009

Y1 - 2009

N2 - We report a proteomics strategy to both identify and quantify cellular target protein interactions with externally introduced ligands. We determined dissociation constants for target proteins interacting with the ligand of interest by combining quantitative mass spectrometry with a defined set of affinity purification experiments. We demonstrate the general utility of this methodology in interaction studies involving small-molecule kinase inhibitors, a tyrosine-phosphorylated peptide and an antibody as affinity ligands.

AB - We report a proteomics strategy to both identify and quantify cellular target protein interactions with externally introduced ligands. We determined dissociation constants for target proteins interacting with the ligand of interest by combining quantitative mass spectrometry with a defined set of affinity purification experiments. We demonstrate the general utility of this methodology in interaction studies involving small-molecule kinase inhibitors, a tyrosine-phosphorylated peptide and an antibody as affinity ligands.

U2 - 10.1038/nmeth.1373

DO - 10.1038/nmeth.1373

M3 - Journal article

C2 - 19749761

SN - 1548-7091

VL - 6

SP - 741

EP - 744

JO - Nature Methods

JF - Nature Methods

IS - 10

ER -