Protocol for ex vivo culture of adult mouse testis tissue using a gel-based approach

Nadia Krarup Knudsen; Christine Hjorth Andreassen; Martin Blomberg Jensen; Anne Jørgensen

doi:10.1016/j.xpro.2025.103731

Protocol for ex vivo culture of adult mouse testis tissue using a gel-based approach

Nadia Krarup Knudsen^*, Christine Hjorth Andreassen, Martin Blomberg Jensen, Anne Jørgensen^*

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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Abstract

Testicular function relies on tightly regulated cell-cell interactions, including Sertoli-Leydig cell communication and Sertoli cells nurturing germ cells. Here, we present a 48-h ex vivo culture protocol for adult mouse testis tissue using a gel-based approach. We describe experimental design, dissection, and examination of treatment-induced outcomes, in particular, stimulatory effects on germ cells. We outline procedures for quantifying apoptosis and germ cell proliferation in the tissue and measurement

Originalsprog	Engelsk
Artikelnummer	103731
Tidsskrift	STAR Protocols
Vol/bind	6
Udgave nummer	2
Antal sider	17
ISSN	2666-1667
DOI	https://doi.org/10.1016/j.xpro.2025.103731
Status	Udgivet - 2025

Bibliografisk note

Publisher Copyright:
© 2025 The Author(s)

Adgang til dokumentet

10.1016/j.xpro.2025.103731Licens: CC BY

FulltextForlagets udgivne version, 5,16 MBLicens: CC BY

Citationsformater

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title = "Protocol for ex vivo culture of adult mouse testis tissue using a gel-based approach",

abstract = "Testicular function relies on tightly regulated cell-cell interactions, including Sertoli-Leydig cell communication and Sertoli cells nurturing germ cells. Here, we present a 48-h ex vivo culture protocol for adult mouse testis tissue using a gel-based approach. We describe experimental design, dissection, and examination of treatment-induced outcomes, in particular, stimulatory effects on germ cells. We outline procedures for quantifying apoptosis and germ cell proliferation in the tissue and measurements of reproductive hormones and paracrine factors secreted to the culture medium. For complete details on the use and execution of this protocol, please refer to Andreassen et al.1",

keywords = "Cell biology, Cell culture, Cell isolation, Model organisms",

author = "{Krarup Knudsen}, Nadia and Andreassen, {Christine Hjorth} and {Blomberg Jensen}, Martin and Anne J{\o}rgensen",

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TY - JOUR

T1 - Protocol for ex vivo culture of adult mouse testis tissue using a gel-based approach

AU - Krarup Knudsen, Nadia

AU - Andreassen, Christine Hjorth

AU - Blomberg Jensen, Martin

AU - Jørgensen, Anne

PY - 2025

Y1 - 2025

N2 - Testicular function relies on tightly regulated cell-cell interactions, including Sertoli-Leydig cell communication and Sertoli cells nurturing germ cells. Here, we present a 48-h ex vivo culture protocol for adult mouse testis tissue using a gel-based approach. We describe experimental design, dissection, and examination of treatment-induced outcomes, in particular, stimulatory effects on germ cells. We outline procedures for quantifying apoptosis and germ cell proliferation in the tissue and measurements of reproductive hormones and paracrine factors secreted to the culture medium. For complete details on the use and execution of this protocol, please refer to Andreassen et al.1

AB - Testicular function relies on tightly regulated cell-cell interactions, including Sertoli-Leydig cell communication and Sertoli cells nurturing germ cells. Here, we present a 48-h ex vivo culture protocol for adult mouse testis tissue using a gel-based approach. We describe experimental design, dissection, and examination of treatment-induced outcomes, in particular, stimulatory effects on germ cells. We outline procedures for quantifying apoptosis and germ cell proliferation in the tissue and measurements of reproductive hormones and paracrine factors secreted to the culture medium. For complete details on the use and execution of this protocol, please refer to Andreassen et al.1

KW - Cell biology

KW - Cell culture

KW - Cell isolation

KW - Model organisms

U2 - 10.1016/j.xpro.2025.103731

DO - 10.1016/j.xpro.2025.103731

M3 - Journal article

C2 - 40208788

AN - SCOPUS:105002134842

SN - 2666-1667

VL - 6

JO - STAR Protocols

JF - STAR Protocols

IS - 2

M1 - 103731

ER -