Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices

Jessica Giacomoni; Mette Habekost; Efrain Cepeda-Prado; Bengt Mattsson; Daniella Rylander Ottosson; Malin Parmar; Janko Kajtez

doi:10.1016/j.xpro.2022.102041

Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices

Jessica Giacomoni, Mette Habekost, Efrain Cepeda-Prado, Bengt Mattsson, Daniella Rylander Ottosson, Malin Parmar, Janko Kajtez

Center for Translational Neuromedicine

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

3 Citationer (Scopus)

16 Downloads (Pure)

Abstract

Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO2 incubators, and handled only at media change.

Originalsprog	Engelsk
Artikelnummer	102041
Tidsskrift	STAR Protocols
Vol/bind	4
Udgave nummer	1
Antal sider	18
ISSN	2666-1667
DOI	https://doi.org/10.1016/j.xpro.2022.102041
Status	Udgivet - 2023

Bibliografisk note

Adgang til dokumentet

10.1016/j.xpro.2022.102041Licens: CC BY

FulltextForlagets udgivne version, 4,02 MBLicens: CC BY

Citationsformater

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AU - Giacomoni, Jessica

AU - Habekost, Mette

AU - Cepeda-Prado, Efrain

AU - Mattsson, Bengt

AU - Ottosson, Daniella Rylander

AU - Parmar, Malin

AU - Kajtez, Janko

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AB - Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO2 incubators, and handled only at media change.

KW - Rats

KW - Animals

KW - Brain/diagnostic imaging

KW - Microscopy, Confocal

U2 - 10.1016/j.xpro.2022.102041

DO - 10.1016/j.xpro.2022.102041

M3 - Journal article

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