Protocol to assess metabolic activity of Pseudomonas aeruginosa by measuring heat flow using isothermal calorimetry

Katrin Beilharz; Kasper Nørskov Kragh; Blaine Fritz; Julius B. Kirkegaard; Tim Tolker-Nielsen; Thomas Bjarnsholt; Mads Lichtenberg

doi:10.1016/j.xpro.2023.102269

Protocol to assess metabolic activity of Pseudomonas aeruginosa by measuring heat flow using isothermal calorimetry

Katrin Beilharz^*, Kasper Nørskov Kragh, Blaine Fritz, Julius B. Kirkegaard, Tim Tolker-Nielsen, Thomas Bjarnsholt, Mads Lichtenberg

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

4 Citationer (Scopus)

35 Downloads (Pure)

Abstract

Here, we present a protocol for assessing metabolic activity of bacterial populations by measuring heat flow using isothermal calorimetry. We outline the steps for preparing the different growth models of Pseudomonas aeruginosa and performing continuous metabolic activity measurements in the calScreener. We detail simple principal component analysis to differentiate between metabolic states of different populations and probabilistic logistic classification to assess resemblance to wild-type bacteria. This protocol for fine-scale metabolic measurement can aid in understanding microbial physiology. For complete details on the use and execution of this protocol, please refer to Lichtenberg et al. (2022).¹

Originalsprog	Engelsk
Artikelnummer	102269
Tidsskrift	STAR Protocols
Vol/bind	4
Udgave nummer	2
ISSN	2666-1667
DOI	https://doi.org/10.1016/j.xpro.2023.102269
Status	Udgivet - 2023

Bibliografisk note

Funding Information:
This study was supported by the Lundbeck Foundation through grants R250-2017-633 (M.L.) and R105-A9791 (T.B.) and the Novo Nordisk Foundation through grants NNF19OC0056411 (T.B.), NNF19OC0054390 (T.B.), and NNF20OC0062047 (J.B.K.), as well as the Independent Research Fund Denmark through grant 9040-00023B (T.T.-N.).

Publisher Copyright:
© 2023 The Authors

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10.1016/j.xpro.2023.102269Licens: CC BY

FulltextForlagets udgivne version, 5,36 MBLicens: CC BY

Citationsformater

Protocol to assess metabolic activity of Pseudomonas aeruginosa by measuring heat flow using isothermal calorimetry. / Beilharz, Katrin; Kragh, Kasper Nørskov; Fritz, Blaine ; Kirkegaard, Julius B.; Tolker-Nielsen, Tim ; Bjarnsholt, Thomas ; Lichtenberg, Mads.

I: STAR Protocols, Bind 4, Nr. 2, 102269, 2023.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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AU - Beilharz, Katrin

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AU - Fritz, Blaine

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AU - Tolker-Nielsen, Tim

AU - Bjarnsholt, Thomas

AU - Lichtenberg, Mads

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AB - Here, we present a protocol for assessing metabolic activity of bacterial populations by measuring heat flow using isothermal calorimetry. We outline the steps for preparing the different growth models of Pseudomonas aeruginosa and performing continuous metabolic activity measurements in the calScreener. We detail simple principal component analysis to differentiate between metabolic states of different populations and probabilistic logistic classification to assess resemblance to wild-type bacteria. This protocol for fine-scale metabolic measurement can aid in understanding microbial physiology. For complete details on the use and execution of this protocol, please refer to Lichtenberg et al. (2022).1

KW - Cell-based Assays

KW - Metabolism

KW - Microbiology

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