Protocol to study molecule exchange between venous blood and CSF in live mice using transcranial bioluminescence imaging

Tina Esmail; Maiken Nedergaard; Felix R.M. Beinlich

doi:10.1016/j.xpro.2023.102652

Protocol to study molecule exchange between venous blood and CSF in live mice using transcranial bioluminescence imaging

Tina Esmail^*, Maiken Nedergaard, Felix R.M. Beinlich

^*Corresponding author af dette arbejde

Center for Translational Neuromedicine

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

1 Citationer (Scopus)

19 Downloads (Pure)

Abstract

In bioluminescence imaging (BLI), the biochemical reaction between a substrate and enzyme triggers light emission upon convergence. Unlike fluorescence imaging, BLI does not require excitation. In this protocol, we utilize the high signal-to-background ratio of the reaction between luciferase and its substrate to study the exchange of molecules between blood and cerebrospinal fluid. We outline steps for skull window thinning, cisterna magna infusion, intravascular retro-orbital injection, and imaging.

Originalsprog	Engelsk
Artikelnummer	102652
Tidsskrift	STAR Protocols
Vol/bind	4
Udgave nummer	4
Antal sider	18
ISSN	2666-1667
DOI	https://doi.org/10.1016/j.xpro.2023.102652
Status	Udgivet - 2023

Bibliografisk note

Funding Information:
We would like to thank D. Xue for graphical support. Funding was provided by Lundbeck Foundation grant R386-2021-165 (M.N.), Novo Nordisk Foundation grant NNF20OC0066419 (M.N.), National Institutes of Health grant R01AT011439 (M.N.), National Institutes of Health grant U19NS128613 (M.N.), US Army Research Office grant MURI W911NF1910280 (M.N.), Human Frontier Science Program grant RGP0036 (M.N.), the Dr. Miriam and Sheldon G. Adelson Medical Research Foundation (M.N.), and Simons Foundation grant 811237 (M.N.). The funding agencies have taken no part in the design of the study, data collection, analysis, interpretation, or in writing of the manuscript.

Publisher Copyright:
© 2023 The Author(s)

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10.1016/j.xpro.2023.102652Licens: CC BY-NC-ND

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Citationsformater

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abstract = "In bioluminescence imaging (BLI), the biochemical reaction between a substrate and enzyme triggers light emission upon convergence. Unlike fluorescence imaging, BLI does not require excitation. In this protocol, we utilize the high signal-to-background ratio of the reaction between luciferase and its substrate to study the exchange of molecules between blood and cerebrospinal fluid. We outline steps for skull window thinning, cisterna magna infusion, intravascular retro-orbital injection, and imaging. For complete details on the use and execution of this protocol, please refer to M{\o}llg{\aa}rd et al. (2023).1",

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AU - Esmail, Tina

AU - Nedergaard, Maiken

AU - Beinlich, Felix R.M.

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Y1 - 2023

N2 - In bioluminescence imaging (BLI), the biochemical reaction between a substrate and enzyme triggers light emission upon convergence. Unlike fluorescence imaging, BLI does not require excitation. In this protocol, we utilize the high signal-to-background ratio of the reaction between luciferase and its substrate to study the exchange of molecules between blood and cerebrospinal fluid. We outline steps for skull window thinning, cisterna magna infusion, intravascular retro-orbital injection, and imaging. For complete details on the use and execution of this protocol, please refer to Møllgård et al. (2023).1

AB - In bioluminescence imaging (BLI), the biochemical reaction between a substrate and enzyme triggers light emission upon convergence. Unlike fluorescence imaging, BLI does not require excitation. In this protocol, we utilize the high signal-to-background ratio of the reaction between luciferase and its substrate to study the exchange of molecules between blood and cerebrospinal fluid. We outline steps for skull window thinning, cisterna magna infusion, intravascular retro-orbital injection, and imaging. For complete details on the use and execution of this protocol, please refer to Møllgård et al. (2023).1

KW - Health Sciences

KW - Microscopy

KW - Neuroscience

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DO - 10.1016/j.xpro.2023.102652

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