TY - JOUR
T1 - PRV-1, erythroid colonies and platelet Mpl are unrelated to thrombosis in essential thrombocythaemia.
AU - Vannucchi, Alessandro M
AU - Pancrazzi, Alessandro
AU - Antonioli, Elisabetta
AU - Guglielmelli, Paola
AU - Balestri, Francesca
AU - Biscardi, Monica
AU - Bulgarelli, Simona
AU - Longo, Giovanni
AU - Graziano, Claudio
AU - Gugliotta, Luigi
AU - Bosi, Alberto
N1 - Keywords: Adult; Blood Platelets; Clone Cells; Dosage Compensation, Genetic; Erythroid Progenitor Cells; Female; Gene Expression; Humans; Isoantigens; Logistic Models; Membrane Glycoproteins; Middle Aged; Myelopoiesis; Predictive Value of Tests; Receptors, Cell Surface; Thrombocythemia, Hemorrhagic; Thrombopoietin; Thrombosis
PY - 2004
Y1 - 2004
N2 - Females with the monoclonal type of essential thrombocythaemia (ET), based on the X-chromosome inactivation pattern (XCIP), have previously been shown to present a higher incidence of thrombosis than polyclonal ones. We aimed to assess correlations between XCIP, thrombosis, and three epigenetic markers of ET, namely PRV-1 overexpression, endogenous erythroid colony (EEC) formation, and reduced platelet Mpl content. Fifty-three (60%) of 88 subjects studied had monoclonal myelopoiesis and presented a 32% incidence of major thrombosis compared with 6% of polyclonal subjects (P = 0.009). The frequency of abnormalities of PRV-1, EEC, or Mpl was similar in monoclonal and polyclonal subjects (respectively, 28%, 48%, 75%, and 37%, 27%, 63%), and none of them correlated with thrombosis. We conclude that the exploited epigenetic markers constitute independent phenotypic variations and are not clustered according to monoclonality of myelopoiesis in ET; none of them could serve as a surrogate marker of thrombotic risk in male subjects with ET.
AB - Females with the monoclonal type of essential thrombocythaemia (ET), based on the X-chromosome inactivation pattern (XCIP), have previously been shown to present a higher incidence of thrombosis than polyclonal ones. We aimed to assess correlations between XCIP, thrombosis, and three epigenetic markers of ET, namely PRV-1 overexpression, endogenous erythroid colony (EEC) formation, and reduced platelet Mpl content. Fifty-three (60%) of 88 subjects studied had monoclonal myelopoiesis and presented a 32% incidence of major thrombosis compared with 6% of polyclonal subjects (P = 0.009). The frequency of abnormalities of PRV-1, EEC, or Mpl was similar in monoclonal and polyclonal subjects (respectively, 28%, 48%, 75%, and 37%, 27%, 63%), and none of them correlated with thrombosis. We conclude that the exploited epigenetic markers constitute independent phenotypic variations and are not clustered according to monoclonality of myelopoiesis in ET; none of them could serve as a surrogate marker of thrombotic risk in male subjects with ET.
U2 - 10.1111/j.1365-2141.2004.05175.x
DO - 10.1111/j.1365-2141.2004.05175.x
M3 - Journal article
C2 - 15461629
VL - 127
SP - 214
EP - 219
JO - British Journal of Haematology
JF - British Journal of Haematology
SN - 0007-1048
IS - 2
ER -