Purification, characterization and ELISA detection of mink immunoglobulins

Cyril Jean-Marie Martel, Bent Aasted

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    Abstract

    This study describes easy purification methods for mink IgG, IgA and IgM immunoglobulins. IgG and IgM were purified from normal mink serum, while IgA was purified from mink bile from healthy animals. By SDS-polyacrylamid-gel-electrophoresis  (SDS-PAGE) and immunoblotting under reducing conditions the estimated molecular weights of the immunoglobulin gamma, alpha and mu heavy chains were found to be 54 kDa, 69 kDa and 83 kDa respectively. The purities of purified IgG, IgM and IgA were estimated by immunoglobulin class specific ELISAs to be more than 90% for IgG and IgM, and more than 80% for IgA.
    OriginalsprogEngelsk
    TidsskriftScientifur
    Vol/bind32
    Udgave nummer1
    Sider (fra-til)33-40
    Antal sider8
    ISSN0105-2403
    StatusUdgivet - 2008

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