Purification of human serum hyaluronidase using chromatofocusing

M Fenger

Purification of human serum hyaluronidase using chromatofocusing

M Fenger

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

10 Citationer (Scopus)

Abstract

Udgivelsesdato: 1982-May-7

Originalsprog	Engelsk
Tidsskrift	Journal of Chromatography A
Vol/bind	240
Udgave nummer	1
Sider (fra-til)	173-9
Antal sider	6
ISSN	0021-9673
Status	Udgivet - 1982

Citationsformater

@article{24c94e71f5d54bfa9c49f0b721199078,

title = "Purification of human serum hyaluronidase using chromatofocusing",

abstract = "A commercial chromatofocusing system was applied to Cohn's fraction III of human serum to purify hyaluronidase (E.C.3.2.1.3.5). The protein that eluted in the pH range 4.7-5.3 was pooled and precipitated by adding ammonium sulphate to 50% saturation. This sequence of fractionation purified hyaluronidase extensively by immunological criteria. It is shown that hyaluronidase is a population of enzymes displaying microheterogeneity. The commercial chromatofocusing system behaved as theoretically expected. The capacity of the gel is 3 mg per ml gel. Any overload will be trapped or precipitated in the gel. The gel is easy to handle and did not deteriorate on repeated use.",

author = "M Fenger",

year = "1982",

language = "English",

volume = "240",

pages = "173--9",

journal = "Journal of Chromatography",

issn = "0301-4770",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Purification of human serum hyaluronidase using chromatofocusing

AU - Fenger, M

PY - 1982

Y1 - 1982

N2 - A commercial chromatofocusing system was applied to Cohn's fraction III of human serum to purify hyaluronidase (E.C.3.2.1.3.5). The protein that eluted in the pH range 4.7-5.3 was pooled and precipitated by adding ammonium sulphate to 50% saturation. This sequence of fractionation purified hyaluronidase extensively by immunological criteria. It is shown that hyaluronidase is a population of enzymes displaying microheterogeneity. The commercial chromatofocusing system behaved as theoretically expected. The capacity of the gel is 3 mg per ml gel. Any overload will be trapped or precipitated in the gel. The gel is easy to handle and did not deteriorate on repeated use.

AB - A commercial chromatofocusing system was applied to Cohn's fraction III of human serum to purify hyaluronidase (E.C.3.2.1.3.5). The protein that eluted in the pH range 4.7-5.3 was pooled and precipitated by adding ammonium sulphate to 50% saturation. This sequence of fractionation purified hyaluronidase extensively by immunological criteria. It is shown that hyaluronidase is a population of enzymes displaying microheterogeneity. The commercial chromatofocusing system behaved as theoretically expected. The capacity of the gel is 3 mg per ml gel. Any overload will be trapped or precipitated in the gel. The gel is easy to handle and did not deteriorate on repeated use.

M3 - Journal article

VL - 240

SP - 173

EP - 179

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0301-4770

IS - 1

ER -