Quorum sensing signals are produced by Aeromonas salmonicida and quorum sensing inhibitors can reduce production of a potential virulence factor

Maria Rasch; Vicky Gaedt Kastbjerg; Jesper Bartholin Bruhn; Inger Dalsgaard; Michael Givskov; Lone Gram

Quorum sensing signals are produced by Aeromonas salmonicida and quorum sensing inhibitors can reduce production of a potential virulence factor

Maria Rasch, Vicky Gaedt Kastbjerg, Jesper Bartholin Bruhn, Inger Dalsgaard, Michael Givskov, Lone Gram

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

27 Citationer (Scopus)

Abstract

Udgivelsesdato: 2007-Dec-13

Originalsprog	Engelsk
Tidsskrift	Diseases of Aquatic Organisms
Vol/bind	78
Udgave nummer	2
Sider (fra-til)	105-13
Antal sider	8
ISSN	0177-5103
Status	Udgivet - 2007
Udgivet eksternt	Ja

Bibliografisk note

Keywords: 4-Butyrolactone; Acyl-Butyrolactones; Aeromonas salmonicida; Animals; Fish Diseases; Fishes; Gene Expression Regulation, Bacterial; Gram-Negative Bacterial Infections; Peptide Hydrolases; Pigments, Biological; Quorum Sensing; Time Factors; Virulence Factors

Citationsformater

Quorum sensing signals are produced by Aeromonas salmonicida and quorum sensing inhibitors can reduce production of a potential virulence factor. / Rasch, Maria; Kastbjerg, Vicky Gaedt; Bruhn, Jesper Bartholin; Dalsgaard, Inger; Givskov, Michael; Gram, Lone.

I: Diseases of Aquatic Organisms, Bind 78, Nr. 2, 2007, s. 105-13.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

@article{283bc5f0fce111ddb219000ea68e967b,

title = "Quorum sensing signals are produced by Aeromonas salmonicida and quorum sensing inhibitors can reduce production of a potential virulence factor",

abstract = "Many pathogens control production of virulence factors by self-produced signals in a process called quorum sensing (QS). We demonstrate that acyl homoserine lactone (AHL) signals, which enable bacteria to express certain phenotypes in relation to cell density, are produced by a wide spectrum of Aeromonas salmonicida strains. All 31 typical strains were AHL producers as were 21 of 26 atypical strains, but on a strain population basis, production of virulence factors such as protease, lipase, A-layer or pigment did not correlate with the production and accumulation of AHLs in the growth medium. Pigment production was only observed in broth under highly aerated conditions. Quorum sensing inhibitors (QSIs) are compounds that specifically block QS systems without affecting bacterial growth and 2 such compounds, sulphur-containing AHL-analogues, reduced production of protease in a typical strain of Aeromonas salmonicida. The most efficient compound N-(heptylsulfanylacetyl)-L-homoserine lactone (HepS-AHL), reduced protease production by a factor of 10. Five extracellular proteases were detected on gelatin-containing sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) gels and 3 of these were completely down regulated by HepS-AHL. Hence, QSIs can curb virulence in some strains and could potentially be pursued as bacterial disease control measures in aquaculture.",

author = "Maria Rasch and Kastbjerg, {Vicky Gaedt} and Bruhn, {Jesper Bartholin} and Inger Dalsgaard and Michael Givskov and Lone Gram",

note = "Keywords: 4-Butyrolactone; Acyl-Butyrolactones; Aeromonas salmonicida; Animals; Fish Diseases; Fishes; Gene Expression Regulation, Bacterial; Gram-Negative Bacterial Infections; Peptide Hydrolases; Pigments, Biological; Quorum Sensing; Time Factors; Virulence Factors",

year = "2007",

language = "English",

volume = "78",

pages = "105--13",

journal = "Diseases of Aquatic Organisms",

issn = "0177-5103",

publisher = "Inter-Research",

number = "2",

}

TY - JOUR

T1 - Quorum sensing signals are produced by Aeromonas salmonicida and quorum sensing inhibitors can reduce production of a potential virulence factor

AU - Rasch, Maria

AU - Kastbjerg, Vicky Gaedt

AU - Bruhn, Jesper Bartholin

AU - Dalsgaard, Inger

AU - Givskov, Michael

AU - Gram, Lone

N1 - Keywords: 4-Butyrolactone; Acyl-Butyrolactones; Aeromonas salmonicida; Animals; Fish Diseases; Fishes; Gene Expression Regulation, Bacterial; Gram-Negative Bacterial Infections; Peptide Hydrolases; Pigments, Biological; Quorum Sensing; Time Factors; Virulence Factors

PY - 2007

Y1 - 2007

N2 - Many pathogens control production of virulence factors by self-produced signals in a process called quorum sensing (QS). We demonstrate that acyl homoserine lactone (AHL) signals, which enable bacteria to express certain phenotypes in relation to cell density, are produced by a wide spectrum of Aeromonas salmonicida strains. All 31 typical strains were AHL producers as were 21 of 26 atypical strains, but on a strain population basis, production of virulence factors such as protease, lipase, A-layer or pigment did not correlate with the production and accumulation of AHLs in the growth medium. Pigment production was only observed in broth under highly aerated conditions. Quorum sensing inhibitors (QSIs) are compounds that specifically block QS systems without affecting bacterial growth and 2 such compounds, sulphur-containing AHL-analogues, reduced production of protease in a typical strain of Aeromonas salmonicida. The most efficient compound N-(heptylsulfanylacetyl)-L-homoserine lactone (HepS-AHL), reduced protease production by a factor of 10. Five extracellular proteases were detected on gelatin-containing sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) gels and 3 of these were completely down regulated by HepS-AHL. Hence, QSIs can curb virulence in some strains and could potentially be pursued as bacterial disease control measures in aquaculture.

AB - Many pathogens control production of virulence factors by self-produced signals in a process called quorum sensing (QS). We demonstrate that acyl homoserine lactone (AHL) signals, which enable bacteria to express certain phenotypes in relation to cell density, are produced by a wide spectrum of Aeromonas salmonicida strains. All 31 typical strains were AHL producers as were 21 of 26 atypical strains, but on a strain population basis, production of virulence factors such as protease, lipase, A-layer or pigment did not correlate with the production and accumulation of AHLs in the growth medium. Pigment production was only observed in broth under highly aerated conditions. Quorum sensing inhibitors (QSIs) are compounds that specifically block QS systems without affecting bacterial growth and 2 such compounds, sulphur-containing AHL-analogues, reduced production of protease in a typical strain of Aeromonas salmonicida. The most efficient compound N-(heptylsulfanylacetyl)-L-homoserine lactone (HepS-AHL), reduced protease production by a factor of 10. Five extracellular proteases were detected on gelatin-containing sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) gels and 3 of these were completely down regulated by HepS-AHL. Hence, QSIs can curb virulence in some strains and could potentially be pursued as bacterial disease control measures in aquaculture.

M3 - Journal article

C2 - 18286807

VL - 78

SP - 105

EP - 113

JO - Diseases of Aquatic Organisms

JF - Diseases of Aquatic Organisms

SN - 0177-5103

IS - 2

ER -