Rat fetuin: distribution of protein and mRNA in embryonic and neonatal rat tissues

O B Terkelsen; W Jahnen-Dechent; Henrik Nielsen; T Moos; E Fink; P Nawratil; W Müller-Esterl; K Møllgård

Rat fetuin: distribution of protein and mRNA in embryonic and neonatal rat tissues

O B Terkelsen, W Jahnen-Dechent, Henrik Nielsen, T Moos, E Fink, P Nawratil, W Müller-Esterl, K Møllgård

Institut for Cellulær og Molekylær Medicin

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

45 Citationer (Scopus)

Abstract

Udgivelsesdato: 1998-Feb

Originalsprog	Engelsk
Tidsskrift	Anatomy and Embryology
Vol/bind	197
Udgave nummer	2
Sider (fra-til)	125-33
Antal sider	8
ISSN	0340-2061
Status	Udgivet - 1998

Bibliografisk note

Keywords: Animals; Animals, Newborn; Antibody Formation; Blotting, Northern; Embryo, Mammalian; Female; Immunoenzyme Techniques; In Situ Hybridization; Mice; RNA, Messenger; Rabbits; Rats; Tissue Distribution; alpha-Fetoproteins

Citationsformater

@article{eb1cba70a75911debc73000ea68e967b,

title = "Rat fetuin: distribution of protein and mRNA in embryonic and neonatal rat tissues",

abstract = "Fetuin is a serum protein widely distributed in the animal kingdom and found in all mammalian species so far investigated. It is mainly a fetal protein, in the sense that the highest concentrations are found in serum and body fluids of embryos and fetuses. In order to elucidate possible biological functions of fetuin, we have studied its synthesis and distribution during the prenatal development of the rat with immunohistochemistry and in situ hybridization. We have isolated fetuin from rat serum and produced an antibody against this protein. In situ hybridization was performed using a 375-nucleotides-long digoxigenin-labeled riboprobe. Fetuin was unevenly distributed in all organ systems during development, with the most pronounced expression at E 10Fetuin is a serum protein widely distributed in the animal kingdom and found in all mammalian species so far investigated. It is mainly a fetal protein, in the sense that the highest concentrations are found in serum and body fluids of embryos and fetuses. In order to elucidate possible biological functions of fetuin, we have studied its synthesis and distribution during the prenatal development of the rat with immunohistochemistry and in situ hybridization. We have isolated fetuin from rat serum and produced an antibody against this protein. In situ hybridization was performed using a 375-nucleotides-long digoxigenin-labeled riboprobe. Fetuin was unevenly distributed in all organ systems during development, with the most pronounced expression at E16-E18. Fetuin expression was present in germinal cell populations, e.g., in the basal layer in the skin, in the germinal cell populations in the brain anlage and the gonads, and it was heavily expressed in the fetal hemopoietic liver. Furthermore, fetuin was expressed in the gastrointestinal epithelium prior to the development of glands and crypts. Fetuin was widely distributed in mesenchymal derived tissues, e.g., bone and muscle. In the developing kidney fetuin was heavily expressed is both mesenchymal condensations and glomerular anlages. Thus, fetuin was located in cells or structures undergoing differentiation and transformation. As fetuin has been shown previously to interfere with hormone signaling of transforming growth factor-beta, insulin and hepatocyte-growth factor, fetuin might be involved in cell differentiation and tissue transformation during the initial histogenesis, i.e., the time period in which cellular phenotypic characteristics are established.",

author = "Terkelsen, {O B} and W Jahnen-Dechent and Henrik Nielsen and T Moos and E Fink and P Nawratil and W M{\"u}ller-Esterl and K M{\o}llg{\aa}rd",

note = "Keywords: Animals; Animals, Newborn; Antibody Formation; Blotting, Northern; Embryo, Mammalian; Female; Immunoenzyme Techniques; In Situ Hybridization; Mice; RNA, Messenger; Rabbits; Rats; Tissue Distribution; alpha-Fetoproteins",

year = "1998",

language = "English",

volume = "197",

pages = "125--33",

journal = "Anatomy and Embryology",

issn = "0177-5154",

publisher = "Springer Verlag",

number = "2",

}

TY - JOUR

T1 - Rat fetuin: distribution of protein and mRNA in embryonic and neonatal rat tissues

AU - Terkelsen, O B

AU - Jahnen-Dechent, W

AU - Nielsen, Henrik

AU - Moos, T

AU - Fink, E

AU - Nawratil, P

AU - Müller-Esterl, W

AU - Møllgård, K

N1 - Keywords: Animals; Animals, Newborn; Antibody Formation; Blotting, Northern; Embryo, Mammalian; Female; Immunoenzyme Techniques; In Situ Hybridization; Mice; RNA, Messenger; Rabbits; Rats; Tissue Distribution; alpha-Fetoproteins

PY - 1998

Y1 - 1998

N2 - Fetuin is a serum protein widely distributed in the animal kingdom and found in all mammalian species so far investigated. It is mainly a fetal protein, in the sense that the highest concentrations are found in serum and body fluids of embryos and fetuses. In order to elucidate possible biological functions of fetuin, we have studied its synthesis and distribution during the prenatal development of the rat with immunohistochemistry and in situ hybridization. We have isolated fetuin from rat serum and produced an antibody against this protein. In situ hybridization was performed using a 375-nucleotides-long digoxigenin-labeled riboprobe. Fetuin was unevenly distributed in all organ systems during development, with the most pronounced expression at E 10Fetuin is a serum protein widely distributed in the animal kingdom and found in all mammalian species so far investigated. It is mainly a fetal protein, in the sense that the highest concentrations are found in serum and body fluids of embryos and fetuses. In order to elucidate possible biological functions of fetuin, we have studied its synthesis and distribution during the prenatal development of the rat with immunohistochemistry and in situ hybridization. We have isolated fetuin from rat serum and produced an antibody against this protein. In situ hybridization was performed using a 375-nucleotides-long digoxigenin-labeled riboprobe. Fetuin was unevenly distributed in all organ systems during development, with the most pronounced expression at E16-E18. Fetuin expression was present in germinal cell populations, e.g., in the basal layer in the skin, in the germinal cell populations in the brain anlage and the gonads, and it was heavily expressed in the fetal hemopoietic liver. Furthermore, fetuin was expressed in the gastrointestinal epithelium prior to the development of glands and crypts. Fetuin was widely distributed in mesenchymal derived tissues, e.g., bone and muscle. In the developing kidney fetuin was heavily expressed is both mesenchymal condensations and glomerular anlages. Thus, fetuin was located in cells or structures undergoing differentiation and transformation. As fetuin has been shown previously to interfere with hormone signaling of transforming growth factor-beta, insulin and hepatocyte-growth factor, fetuin might be involved in cell differentiation and tissue transformation during the initial histogenesis, i.e., the time period in which cellular phenotypic characteristics are established.

AB - Fetuin is a serum protein widely distributed in the animal kingdom and found in all mammalian species so far investigated. It is mainly a fetal protein, in the sense that the highest concentrations are found in serum and body fluids of embryos and fetuses. In order to elucidate possible biological functions of fetuin, we have studied its synthesis and distribution during the prenatal development of the rat with immunohistochemistry and in situ hybridization. We have isolated fetuin from rat serum and produced an antibody against this protein. In situ hybridization was performed using a 375-nucleotides-long digoxigenin-labeled riboprobe. Fetuin was unevenly distributed in all organ systems during development, with the most pronounced expression at E 10Fetuin is a serum protein widely distributed in the animal kingdom and found in all mammalian species so far investigated. It is mainly a fetal protein, in the sense that the highest concentrations are found in serum and body fluids of embryos and fetuses. In order to elucidate possible biological functions of fetuin, we have studied its synthesis and distribution during the prenatal development of the rat with immunohistochemistry and in situ hybridization. We have isolated fetuin from rat serum and produced an antibody against this protein. In situ hybridization was performed using a 375-nucleotides-long digoxigenin-labeled riboprobe. Fetuin was unevenly distributed in all organ systems during development, with the most pronounced expression at E16-E18. Fetuin expression was present in germinal cell populations, e.g., in the basal layer in the skin, in the germinal cell populations in the brain anlage and the gonads, and it was heavily expressed in the fetal hemopoietic liver. Furthermore, fetuin was expressed in the gastrointestinal epithelium prior to the development of glands and crypts. Fetuin was widely distributed in mesenchymal derived tissues, e.g., bone and muscle. In the developing kidney fetuin was heavily expressed is both mesenchymal condensations and glomerular anlages. Thus, fetuin was located in cells or structures undergoing differentiation and transformation. As fetuin has been shown previously to interfere with hormone signaling of transforming growth factor-beta, insulin and hepatocyte-growth factor, fetuin might be involved in cell differentiation and tissue transformation during the initial histogenesis, i.e., the time period in which cellular phenotypic characteristics are established.

M3 - Journal article

C2 - 9497155

VL - 197

SP - 125

EP - 133

JO - Anatomy and Embryology

JF - Anatomy and Embryology

SN - 0177-5154

IS - 2

ER -