Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules

Matteo Stravalaci; Isabel Pagani; Elvezia Maria Paraboschi; Mattia Pedotti; Andrea Doni; Francesco Scavello; Sarah N. Mapelli; Marina Sironi; Chiara Perucchini; Luca Varani; Milos Matkovic; Andrea Cavalli; Daniela Cesana; Pierangela Gallina; Nicoletta Pedemonte; Valeria Capurro; Nicola Clementi; Nicasio Mancini; Pietro Invernizzi; Rafael Bayarri-Olmos; Peter Garred; Rino Rappuoli; Stefano Duga; Barbara Bottazzi; Mariagrazia Uguccioni; Rosanna Asselta; Elisa Vicenzi; Alberto Mantovani; Cecilia Garlanda

doi:10.1038/s41590-021-01114-w

Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules

Matteo Stravalaci, Isabel Pagani, Elvezia Maria Paraboschi, Mattia Pedotti, Andrea Doni, Francesco Scavello, Sarah N. Mapelli, Marina Sironi, Chiara Perucchini, Luca Varani, Milos Matkovic, Andrea Cavalli, Daniela Cesana, Pierangela Gallina, Nicoletta Pedemonte, Valeria Capurro, Nicola Clementi, Nicasio Mancini, Pietro Invernizzi, Rafael Bayarri-OlmosPeter Garred, Rino Rappuoli, Stefano Duga, Barbara Bottazzi, Mariagrazia Uguccioni, Rosanna Asselta, Elisa Vicenzi, Alberto Mantovani, Cecilia Garlanda

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

114 Citationer (Scopus)

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Abstract

The humoral arm of innate immunity includes diverse molecules with antibody-like functions, some of which serve as disease severity biomarkers in coronavirus disease 2019 (COVID-19). The present study was designed to conduct a systematic investigation of the interaction of human humoral fluid-phase pattern recognition molecules (PRMs) with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Of 12 PRMs tested, the long pentraxin 3 (PTX3) and mannose-binding lectin (MBL) bound the viral nucleocapsid and spike proteins, respectively. MBL bound trimeric spike protein, including that of variants of concern (VoC), in a glycan-dependent manner and inhibited SARS-CoV-2 in three in vitro models. Moreover, after binding to spike protein, MBL activated the lectin pathway of complement activation. Based on retention of glycosylation sites and modeling, MBL was predicted to recognize the Omicron VoC. Genetic polymorphisms at the MBL2 locus were associated with disease severity. These results suggest that selected humoral fluid-phase PRMs can play an important role in resistance to, and pathogenesis of, COVID-19, a finding with translational implications.

Stravalaci et al. examined recognition of SARS-CoV-2 by human soluble innate pattern recognition receptor. They report that pentraxin 3 and mannose-binding protein recognize viral nucleoprotein and spike, respectively. Mannose-binding lectin has antiviral activity, and human genetic polymorphisms of MBL2 are associated with more severe COVID-19.

Originalsprog	Engelsk
Tidsskrift	Nature Immunology
Vol/bind	23
Udgave nummer	2
Sider (fra-til)	275-286
Antal sider	25
ISSN	1529-2908
DOI	https://doi.org/10.1038/s41590-021-01114-w
Status	Udgivet - feb. 2022

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10.1038/s41590-021-01114-w

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Stravalaci, M., Pagani, I., Paraboschi, E. M., Pedotti, M., Doni, A., Scavello, F., Mapelli, S. N., Sironi, M., Perucchini, C., Varani, L., Matkovic, M., Cavalli, A., Cesana, D., Gallina, P., Pedemonte, N., Capurro, V., Clementi, N., Mancini, N., Invernizzi, P., ... Garlanda, C. (2022). Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules. Nature Immunology, 23(2), 275-286. https://doi.org/10.1038/s41590-021-01114-w

Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules. / Stravalaci, Matteo; Pagani, Isabel; Paraboschi, Elvezia Maria; Pedotti, Mattia; Doni, Andrea; Scavello, Francesco; Mapelli, Sarah N.; Sironi, Marina; Perucchini, Chiara; Varani, Luca; Matkovic, Milos; Cavalli, Andrea; Cesana, Daniela; Gallina, Pierangela; Pedemonte, Nicoletta; Capurro, Valeria; Clementi, Nicola; Mancini, Nicasio; Invernizzi, Pietro; Bayarri-Olmos, Rafael; Garred, Peter; Rappuoli, Rino; Duga, Stefano; Bottazzi, Barbara; Uguccioni, Mariagrazia; Asselta, Rosanna; Vicenzi, Elisa; Mantovani, Alberto; Garlanda, Cecilia.

I: Nature Immunology, Bind 23, Nr. 2, 02.2022, s. 275-286.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Stravalaci, M, Pagani, I, Paraboschi, EM, Pedotti, M, Doni, A, Scavello, F, Mapelli, SN, Sironi, M, Perucchini, C, Varani, L, Matkovic, M, Cavalli, A, Cesana, D, Gallina, P, Pedemonte, N, Capurro, V, Clementi, N, Mancini, N, Invernizzi, P, Bayarri-Olmos, R, Garred, P, Rappuoli, R, Duga, S, Bottazzi, B, Uguccioni, M, Asselta, R, Vicenzi, E, Mantovani, A & Garlanda, C 2022, 'Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules', Nature Immunology, bind 23, nr. 2, s. 275-286. https://doi.org/10.1038/s41590-021-01114-w

Stravalaci, Matteo ; Pagani, Isabel ; Paraboschi, Elvezia Maria ; Pedotti, Mattia ; Doni, Andrea ; Scavello, Francesco ; Mapelli, Sarah N. ; Sironi, Marina ; Perucchini, Chiara ; Varani, Luca ; Matkovic, Milos ; Cavalli, Andrea ; Cesana, Daniela ; Gallina, Pierangela ; Pedemonte, Nicoletta ; Capurro, Valeria ; Clementi, Nicola ; Mancini, Nicasio ; Invernizzi, Pietro ; Bayarri-Olmos, Rafael ; Garred, Peter ; Rappuoli, Rino ; Duga, Stefano ; Bottazzi, Barbara ; Uguccioni, Mariagrazia ; Asselta, Rosanna ; Vicenzi, Elisa ; Mantovani, Alberto ; Garlanda, Cecilia. / Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules. I: Nature Immunology. 2022 ; Bind 23, Nr. 2. s. 275-286.

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AU - Rappuoli, Rino

AU - Duga, Stefano

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