@article{25f70940ebce11ddbf70000ea68e967b,
title = "Relative contribution of {"}determinant selection{"} and {"}holes in the T-cell repertoire{"} to T-cell responses",
abstract = "Using BALB/c and CBA/J mice, the I-region associated (Ia) binding capacity and T-cell immunogenicity of a panel of 14 overlapping peptides that span the entire sequence of the protein staphylococcal nuclease (Nase) was examined to evaluate major histocompatibility gene complex (MHC) control of T-cell responses. Ia binding and Ia-restricted T-cell immunogenicity could be determined for a total of 54 peptide-MHC combinations. Only 30% of the 54 instances examined involved detectable Ia binding, but they represented almost all (12 of 13) of the immune responses found. However, binding to Ia was not sufficient to ensure T-cell immunogenicity, since only 70% of the binding events were productive--i.e., were associated with an immune response. Thus, Ia molecules have the expected characteristics of a highly permissive capacity for antigen interaction that allows them to function as restriction elements for a large universe of antigens. On the other hand, since the Ia molecules cannot distinguish between self and non-self, not all antigen-Ia interactions would be permitted to elicit a T-cell response. It appears that both Ia binding ({"}determinant selection{"}) and T-cell repertoire act in concert to define the immune response status of an individual toward any particular T-cell epitope.",
author = "Schaeffer, {E B} and A Sette and Johnson, {D L} and Bekoff, {M C} and Smith, {J A} and Grey, {H M} and S Buus",
note = "Keywords: Animals; Cell Line; Epitopes; Histocompatibility Antigens Class II; Immunity, Cellular; Lymphoma; Major Histocompatibility Complex; Mice; Mice, Inbred BALB C; Mice, Inbred CBA; Mice, Inbred Strains; Micrococcal Nuclease; Peptides; T-Lymphocytes",
year = "1989",
language = "English",
volume = "86",
pages = "4649--53",
journal = "Proceedings of the National Academy of Science of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "12",
}