Reliable reference miRNAs for quantitative gene expression analysis of stress responses in Caenorhabditis elegans

Konstantinos Kagias; Agnieszka Podolska; Roger David John Pocock

doi:10.1186/1471-2164-15-222

Reliable reference miRNAs for quantitative gene expression analysis of stress responses in Caenorhabditis elegans

Konstantinos Kagias, Agnieszka Podolska, Roger David John Pocock

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

14 Citationer (Scopus)

Abstract

Quantitative real-time PCR (qPCR) has become the "gold standard" for measuring expression levels of individual miRNAs. However, little is known about the validity of reference miRNAs, the improper use of which can result in misleading interpretation of data.

Originalsprog	Engelsk
Tidsskrift	BMC Genomics
Vol/bind	15
Udgave nummer	1
Sider (fra-til)	222
ISSN	1471-2164
DOI	https://doi.org/10.1186/1471-2164-15-222
Status	Udgivet - 21 mar. 2014

Adgang til dokumentet

10.1186/1471-2164-15-222

Citationsformater

@article{0ea0c82530e342feb932149d58573f48,

title = "Reliable reference miRNAs for quantitative gene expression analysis of stress responses in Caenorhabditis elegans",

abstract = "Quantitative real-time PCR (qPCR) has become the {"}gold standard{"} for measuring expression levels of individual miRNAs. However, little is known about the validity of reference miRNAs, the improper use of which can result in misleading interpretation of data.",

author = "Konstantinos Kagias and Agnieszka Podolska and Pocock, {Roger David John}",

year = "2014",

month = mar,

day = "21",

doi = "10.1186/1471-2164-15-222",

language = "English",

volume = "15",

pages = "222",

journal = "BMC Genomics",

issn = "1471-2164",

publisher = "BioMed Central Ltd.",

number = "1",

}

TY - JOUR

T1 - Reliable reference miRNAs for quantitative gene expression analysis of stress responses in Caenorhabditis elegans

AU - Kagias, Konstantinos

AU - Podolska, Agnieszka

AU - Pocock, Roger David John

PY - 2014/3/21

Y1 - 2014/3/21

N2 - Quantitative real-time PCR (qPCR) has become the "gold standard" for measuring expression levels of individual miRNAs. However, little is known about the validity of reference miRNAs, the improper use of which can result in misleading interpretation of data.

AB - Quantitative real-time PCR (qPCR) has become the "gold standard" for measuring expression levels of individual miRNAs. However, little is known about the validity of reference miRNAs, the improper use of which can result in misleading interpretation of data.

U2 - 10.1186/1471-2164-15-222

DO - 10.1186/1471-2164-15-222

M3 - Journal article

C2 - 24656064

SN - 1471-2164

VL - 15

SP - 222

JO - BMC Genomics

JF - BMC Genomics

IS - 1

ER -