Restriction fragment length polymorphism (RFLP) of two HLA-B-associated transcripts (BATs) genes in healthy Danes

L Fugger; N Morling; L P Ryder; B K Jakobsen; A Svejgaard; T Spies; J L Strominger

Restriction fragment length polymorphism (RFLP) of two HLA-B-associated transcripts (BATs) genes in healthy Danes

L Fugger, N Morling, L P Ryder, B K Jakobsen, A Svejgaard, T Spies, J L Strominger

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

8 Citationer (Scopus)

Abstract

Udgivelsesdato: 1990-Aug

Originalsprog	Engelsk
Tidsskrift	HLA
Vol/bind	36
Udgave nummer	2
Sider (fra-til)	57-61
Antal sider	4
ISSN	2059-2302
Status	Udgivet - 1990

Bibliografisk note

Keywords: DNA Probes, HLA; Denmark; Deoxyribonucleases, Type II Site-Specific; HLA Antigens; HLA-B Antigens; Humans; Polymorphism, Restriction Fragment Length; Tumor Necrosis Factor-alpha

Citationsformater

@article{84751b80e4be11deba73000ea68e967b,

title = "Restriction fragment length polymorphism (RFLP) of two HLA-B-associated transcripts (BATs) genes in healthy Danes",

abstract = "The restriction fragment length polymorphism (RFLP) of the two human HLA-B-associated transcripts (BATs) genes, BAT1 and BAT2, was investigated using 5 different restriction enzymes and two human BAT1 and BAT2 cDNA probes. Two of the enzymes, NcoI and RsaI, revealed polymorphic patterns which were investigated in healthy Danes. The cDNA/restriction enzyme combination BAT1/NcoI identifies polymorphic bands at 12 kb, 8 kb, 2.5 kb, and 1.1 kb, while the BAT2/RsaI combination identifies polymorphic bands at 3.3 kb, 2.7 kb, 2.3 kb, and 0.9 kb. The frequencies of these markers were determined in 90 unrelated Danes. Co-dominant segregation and allelic behavior was seen for the BAT1/NcoI 12 kb and 8 kb bands and the BAT2/RsaI 2.7 kb and 2.3 kb bands, respectively. It is possible that the BAT2/RsaI 3.3 kb band represents a rare allele of the BAT2/RsaI system. The BAT2/RsaI 2.3 kb marker was strongly negatively associated with HLA-B8 and HLA-DR3 while there were no strong associations between the BAT1 and BAT2 markers, but the BAT2/RsaI 2.7 kb marker was strongly positively associated with the TNF alpha/NcoI 5.5 kb marker, which in turn is positively associated with HLA-B8 and HLA-DR3. The negative associations between the BAT2/RsaI 2.3 kb marker and HLA-B8 and HLA-DR3 raise the question as to whether this BAT allele may play a role in protecting against certain autoimmune diseases.",

author = "L Fugger and N Morling and Ryder, {L P} and Jakobsen, {B K} and A Svejgaard and T Spies and Strominger, {J L}",

note = "Keywords: DNA Probes, HLA; Denmark; Deoxyribonucleases, Type II Site-Specific; HLA Antigens; HLA-B Antigens; Humans; Polymorphism, Restriction Fragment Length; Tumor Necrosis Factor-alpha",

year = "1990",

language = "English",

volume = "36",

pages = "57--61",

journal = "HLA",

issn = "2059-2302",

publisher = "Wiley",

number = "2",

}

TY - JOUR

T1 - Restriction fragment length polymorphism (RFLP) of two HLA-B-associated transcripts (BATs) genes in healthy Danes

AU - Fugger, L

AU - Morling, N

AU - Ryder, L P

AU - Jakobsen, B K

AU - Svejgaard, A

AU - Spies, T

AU - Strominger, J L

N1 - Keywords: DNA Probes, HLA; Denmark; Deoxyribonucleases, Type II Site-Specific; HLA Antigens; HLA-B Antigens; Humans; Polymorphism, Restriction Fragment Length; Tumor Necrosis Factor-alpha

PY - 1990

Y1 - 1990

N2 - The restriction fragment length polymorphism (RFLP) of the two human HLA-B-associated transcripts (BATs) genes, BAT1 and BAT2, was investigated using 5 different restriction enzymes and two human BAT1 and BAT2 cDNA probes. Two of the enzymes, NcoI and RsaI, revealed polymorphic patterns which were investigated in healthy Danes. The cDNA/restriction enzyme combination BAT1/NcoI identifies polymorphic bands at 12 kb, 8 kb, 2.5 kb, and 1.1 kb, while the BAT2/RsaI combination identifies polymorphic bands at 3.3 kb, 2.7 kb, 2.3 kb, and 0.9 kb. The frequencies of these markers were determined in 90 unrelated Danes. Co-dominant segregation and allelic behavior was seen for the BAT1/NcoI 12 kb and 8 kb bands and the BAT2/RsaI 2.7 kb and 2.3 kb bands, respectively. It is possible that the BAT2/RsaI 3.3 kb band represents a rare allele of the BAT2/RsaI system. The BAT2/RsaI 2.3 kb marker was strongly negatively associated with HLA-B8 and HLA-DR3 while there were no strong associations between the BAT1 and BAT2 markers, but the BAT2/RsaI 2.7 kb marker was strongly positively associated with the TNF alpha/NcoI 5.5 kb marker, which in turn is positively associated with HLA-B8 and HLA-DR3. The negative associations between the BAT2/RsaI 2.3 kb marker and HLA-B8 and HLA-DR3 raise the question as to whether this BAT allele may play a role in protecting against certain autoimmune diseases.

AB - The restriction fragment length polymorphism (RFLP) of the two human HLA-B-associated transcripts (BATs) genes, BAT1 and BAT2, was investigated using 5 different restriction enzymes and two human BAT1 and BAT2 cDNA probes. Two of the enzymes, NcoI and RsaI, revealed polymorphic patterns which were investigated in healthy Danes. The cDNA/restriction enzyme combination BAT1/NcoI identifies polymorphic bands at 12 kb, 8 kb, 2.5 kb, and 1.1 kb, while the BAT2/RsaI combination identifies polymorphic bands at 3.3 kb, 2.7 kb, 2.3 kb, and 0.9 kb. The frequencies of these markers were determined in 90 unrelated Danes. Co-dominant segregation and allelic behavior was seen for the BAT1/NcoI 12 kb and 8 kb bands and the BAT2/RsaI 2.7 kb and 2.3 kb bands, respectively. It is possible that the BAT2/RsaI 3.3 kb band represents a rare allele of the BAT2/RsaI system. The BAT2/RsaI 2.3 kb marker was strongly negatively associated with HLA-B8 and HLA-DR3 while there were no strong associations between the BAT1 and BAT2 markers, but the BAT2/RsaI 2.7 kb marker was strongly positively associated with the TNF alpha/NcoI 5.5 kb marker, which in turn is positively associated with HLA-B8 and HLA-DR3. The negative associations between the BAT2/RsaI 2.3 kb marker and HLA-B8 and HLA-DR3 raise the question as to whether this BAT allele may play a role in protecting against certain autoimmune diseases.

M3 - Journal article

C2 - 1980160

VL - 36

SP - 57

EP - 61

JO - HLA

JF - HLA

SN - 2059-2302

IS - 2

ER -