TY - JOUR
T1 - Review of structural design guiding the development of lipid nanoparticles for nucleic acid delivery
AU - Cárdenas, Marité
AU - Campbell, Richard A.
AU - Yanez Arteta, Marianna
AU - Lawrence, M. Jayne
AU - Sebastiani, Federica
N1 - Funding Information:
M.Y.A. would like to acknowledge Lennart Lindfors for his guidance in the LNP development field as well as reviewing this manuscript. M.C. thanks the Swedish Research Council grant numbers 2018-03990 , 2018-04833 , 2022-03322 and the IKUR Strategy under the collaboration agreement between Ikerbasque Foundation and Biofisika Bizkaia Fundazioa (FBB) on behalf of the Department of Education of the Basque Government. F.S. thanks Politecnico di Milano for funding through MSCA Seal of Excellence fellowship.
Publisher Copyright:
© 2023
PY - 2023/8
Y1 - 2023/8
N2 - Lipid nanoparticles (LNPs) are the most versatile and successful gene delivery systems, notably highlighted by their use in vaccines against COVID-19. LNPs have a well-defined core–shell structure, each region with its own distinctive compositions, suited for a wide range of in vivo delivery applications. Here, we discuss how a detailed knowledge of LNP structure can guide LNP formulation to improve the efficiency of delivery of their nucleic acid payload. Perspectives are detailed on how LNP structural design can guide more efficient nucleic acid transfection. Views on key physical characterization techniques needed for such developments are outlined including opinions on biophysical approaches both correlating structure with functionality in biological fluids and improving their ability to escape the endosome and deliver they payload.
AB - Lipid nanoparticles (LNPs) are the most versatile and successful gene delivery systems, notably highlighted by their use in vaccines against COVID-19. LNPs have a well-defined core–shell structure, each region with its own distinctive compositions, suited for a wide range of in vivo delivery applications. Here, we discuss how a detailed knowledge of LNP structure can guide LNP formulation to improve the efficiency of delivery of their nucleic acid payload. Perspectives are detailed on how LNP structural design can guide more efficient nucleic acid transfection. Views on key physical characterization techniques needed for such developments are outlined including opinions on biophysical approaches both correlating structure with functionality in biological fluids and improving their ability to escape the endosome and deliver they payload.
KW - Cationic ionizable lipids
KW - Lipid nanoparticles
KW - Nucleic acid delicery
KW - Structure-function
U2 - 10.1016/j.cocis.2023.101705
DO - 10.1016/j.cocis.2023.101705
M3 - Review
AN - SCOPUS:85162178818
SN - 1359-0294
VL - 66
JO - Current Opinion in Colloid and Interface Science
JF - Current Opinion in Colloid and Interface Science
M1 - 101705
ER -