RiboMeth-seq: Profiling of 2'-O-Me in RNA

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Abstract

RiboMeth-seq is a sequencing-based method for mapping and quantitation of one of the most abundant RNA modifications, ribose methylation. It is based on a simple chemical principle, namely the several orders of magnitude difference in nucleophilicity of a 2'-OH and a 2'-O-Me. Thus, the method combines alkaline fragmentation and a specialized library construction protocol based on 5'-OH and 2',3' cyclic phosphate ends to prepare RNA for sequencing. The read-ends of library fragments are used for mapping with nucleotide resolution and calculation of the fraction of molecules methylated at the 2'-O-Me sites.

OriginalsprogEngelsk
TitelRNA Methylation
Antal sider21
Vol/bind1562
ForlagSpringer
Publikationsdato2017
Sider189-209
ISBN (Trykt)978-1-4939-6805-3
ISBN (Elektronisk)978-1-4939-6807-7
DOI
StatusUdgivet - 2017
NavnMethods in Molecular Biology
Vol/bind1562
ISSN1064-3745

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