Ribotyping for differentiating Flavobacterium meningosepticum isolates from clinical and environmental sources.

H Colding; J Bangsborg; N E Fiehn; T Bennekov; B Bruun

Ribotyping for differentiating Flavobacterium meningosepticum isolates from clinical and environmental sources.

H Colding, J Bangsborg, N E Fiehn, T Bennekov, B Bruun

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

27 Citationer (Scopus)

Abstract

Udgivelsesdato: 1994-Feb

Originalsprog	Engelsk
Tidsskrift	Journal of Clinical Microbiology
Vol/bind	32
Udgave nummer	2
Sider (fra-til)	501-5
Antal sider	4
ISSN	0095-1137
Status	Udgivet - 1994

Bibliografisk note

Keywords: Bacteremia; Bacterial Typing Techniques; Cross Infection; DNA Restriction Enzymes; DNA, Bacterial; Denmark; Disease Outbreaks; Environmental Microbiology; Flavobacterium; Gram-Negative Bacterial Infections; Humans; Meningitis, Bacterial; Nucleic Acid Hybridization

Citationsformater

@article{8efb4300b55211ddb04f000ea68e967b,

title = "Ribotyping for differentiating Flavobacterium meningosepticum isolates from clinical and environmental sources.",

abstract = "On the basis of DNA-DNA hybridization data, two main genomic relatedness groups (I and II) have been reported for a geographically varied collection of 52 strains of Flavobacterium meningosepticum. Herein, we have shown that genomic group II can be further divided into four subgroups (II:1 to II:4). To examine the taxonomic relevance of the ribosomal patterns of the 52 F. meningosepticum strains, the patterns were compared with existing DNA-DNA hybridization data with restriction enzymes PstI and HindIII. Ribotyping of the 52 F. meningosepticum strains showed banding patterns that could identify them correctly to one of the five genomic groups or subgroups. To assess the value of ribotyping for the interpretation of epidemiological data, the discriminatory power of the method was investigated for the 52 F. meningosepticum strains. With one to four restriction enzymes (PstI, HindIII, ClaI, EcoRI), a discriminatory index of 0.95 to 0.97 was found. The value of ribotyping in an epidemiological setting was assessed for three clinical isolates of F. meningosepticum from an outbreak of meningitis and bacteremia in the neonatal intensive care unit, Rigshospitalet, Copenhagen, Denmark. The three clinical isolates were shown to belong to the same ribotype, characteristic of genomic subgroup II:1. This ribotyping method will prove to be a useful tool for epidemiological studies concerning F. meningosepticum in the future.",

author = "H Colding and J Bangsborg and Fiehn, {N E} and T Bennekov and B Bruun",

note = "Keywords: Bacteremia; Bacterial Typing Techniques; Cross Infection; DNA Restriction Enzymes; DNA, Bacterial; Denmark; Disease Outbreaks; Environmental Microbiology; Flavobacterium; Gram-Negative Bacterial Infections; Humans; Meningitis, Bacterial; Nucleic Acid Hybridization",

year = "1994",

language = "English",

volume = "32",

pages = "501--5",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "2",

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TY - JOUR

T1 - Ribotyping for differentiating Flavobacterium meningosepticum isolates from clinical and environmental sources.

AU - Colding, H

AU - Bangsborg, J

AU - Fiehn, N E

AU - Bennekov, T

AU - Bruun, B

N1 - Keywords: Bacteremia; Bacterial Typing Techniques; Cross Infection; DNA Restriction Enzymes; DNA, Bacterial; Denmark; Disease Outbreaks; Environmental Microbiology; Flavobacterium; Gram-Negative Bacterial Infections; Humans; Meningitis, Bacterial; Nucleic Acid Hybridization

PY - 1994

Y1 - 1994

N2 - On the basis of DNA-DNA hybridization data, two main genomic relatedness groups (I and II) have been reported for a geographically varied collection of 52 strains of Flavobacterium meningosepticum. Herein, we have shown that genomic group II can be further divided into four subgroups (II:1 to II:4). To examine the taxonomic relevance of the ribosomal patterns of the 52 F. meningosepticum strains, the patterns were compared with existing DNA-DNA hybridization data with restriction enzymes PstI and HindIII. Ribotyping of the 52 F. meningosepticum strains showed banding patterns that could identify them correctly to one of the five genomic groups or subgroups. To assess the value of ribotyping for the interpretation of epidemiological data, the discriminatory power of the method was investigated for the 52 F. meningosepticum strains. With one to four restriction enzymes (PstI, HindIII, ClaI, EcoRI), a discriminatory index of 0.95 to 0.97 was found. The value of ribotyping in an epidemiological setting was assessed for three clinical isolates of F. meningosepticum from an outbreak of meningitis and bacteremia in the neonatal intensive care unit, Rigshospitalet, Copenhagen, Denmark. The three clinical isolates were shown to belong to the same ribotype, characteristic of genomic subgroup II:1. This ribotyping method will prove to be a useful tool for epidemiological studies concerning F. meningosepticum in the future.

AB - On the basis of DNA-DNA hybridization data, two main genomic relatedness groups (I and II) have been reported for a geographically varied collection of 52 strains of Flavobacterium meningosepticum. Herein, we have shown that genomic group II can be further divided into four subgroups (II:1 to II:4). To examine the taxonomic relevance of the ribosomal patterns of the 52 F. meningosepticum strains, the patterns were compared with existing DNA-DNA hybridization data with restriction enzymes PstI and HindIII. Ribotyping of the 52 F. meningosepticum strains showed banding patterns that could identify them correctly to one of the five genomic groups or subgroups. To assess the value of ribotyping for the interpretation of epidemiological data, the discriminatory power of the method was investigated for the 52 F. meningosepticum strains. With one to four restriction enzymes (PstI, HindIII, ClaI, EcoRI), a discriminatory index of 0.95 to 0.97 was found. The value of ribotyping in an epidemiological setting was assessed for three clinical isolates of F. meningosepticum from an outbreak of meningitis and bacteremia in the neonatal intensive care unit, Rigshospitalet, Copenhagen, Denmark. The three clinical isolates were shown to belong to the same ribotype, characteristic of genomic subgroup II:1. This ribotyping method will prove to be a useful tool for epidemiological studies concerning F. meningosepticum in the future.

M3 - Journal article

C2 - 8150962

SN - 0095-1137

VL - 32

SP - 501

EP - 505

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 2

ER -