Secretory endometrium highly expresses urocortin messenger RNA and peptide: possible role in the decidualization process.

M Torricelli; G De Falco; P Florio; M Rossi; E Leucci; P Viganò; L Leoncini; F Petraglia

doi:10.1093/humrep/del331

Secretory endometrium highly expresses urocortin messenger RNA and peptide: possible role in the decidualization process.

M Torricelli, G De Falco, P Florio, M Rossi, E Leucci, P Viganò, L Leoncini, F Petraglia

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

26 Citationer (Scopus)

Abstract

Udgivelsesdato: 2007-Jan

Originalsprog	Engelsk
Tidsskrift	Human Reproduction
Vol/bind	22
Udgave nummer	1
Sider (fra-til)	92-6
Antal sider	4
ISSN	0268-1161
DOI	https://doi.org/10.1093/humrep/del331
Status	Udgivet - 2006
Udgivet eksternt	Ja

Bibliografisk note

Keywords: Adult; Corticotropin-Releasing Hormone; Cyclic AMP; Decidua; Endometrium; Estradiol; Female; Gene Expression; Humans; Medroxyprogesterone 17-Acetate; Menstrual Cycle; Prolactin; RNA, Messenger; Stromal Cells; Urocortins

Adgang til dokumentet

10.1093/humrep/del331

Citationsformater

@article{83e34e70527b11dd8d9f000ea68e967b,

title = "Secretory endometrium highly expresses urocortin messenger RNA and peptide: possible role in the decidualization process.",

abstract = "BACKGROUND: Urocortin (UCN) gene expression and synthesis have been reported in epithelial and stromal cells of the human endometrium. In this study we evaluated (i) UCN messenger RNA (mRNA) expression and peptide production in uterine specimens collected throughout the endometrial cycle, (ii) UCN secretion after decidualization of cultured human endometrial stromal cells (HESCs) and (iii) the effect of UCN on endometrial decidualization. METHODS: HESCs were isolated from samples of human endometrium collected from healthy patients with normal menstrual cycle and cultured in presence of cAMP, 17-beta-estradiol (E(2)) + medroxyprogesterone acetate (MPA) and UCN. UCN levels were measured in endometrial extracts by an enzyme immunoassay, and changes of endometrial UCN mRNA expression were measured by RT-PCR analysis. RESULTS: UCN peptide concentrations and mRNA expression were highest in the secretory phase of the menstrual cycle (P < 0.001, late secretory versus early and late proliferative phase) and higher in the late than the early secretory phase (P < 0.01). After decidualization of HESC with cAMP or E(2) + MPA, UCN levels rose in parallel with prolactin concentrations by days 6 (P < 0.01, for all). Finally, the addition of UCN to HESCs, with or without E(2) + MPA, induced the release of prolactin. CONCLUSIONS: The evidence that (i) UCN is highly expressed in the secretory phase of the endometrial cycle; (ii) cAMP and E(2) + MPA modulate secretion of UCN and (iii) UCN induces HESCs decidualization together suggest a possible role for UCN in endometrial physiology.",

author = "M Torricelli and {De Falco}, G and P Florio and M Rossi and E Leucci and P Vigan{\`o} and L Leoncini and F Petraglia",

note = "Keywords: Adult; Corticotropin-Releasing Hormone; Cyclic AMP; Decidua; Endometrium; Estradiol; Female; Gene Expression; Humans; Medroxyprogesterone 17-Acetate; Menstrual Cycle; Prolactin; RNA, Messenger; Stromal Cells; Urocortins",

year = "2006",

doi = "10.1093/humrep/del331",

language = "English",

volume = "22",

pages = "92--6",

journal = "Human Reproduction",

issn = "0268-1161",

publisher = "Oxford Academic",

number = "1",

}

TY - JOUR

T1 - Secretory endometrium highly expresses urocortin messenger RNA and peptide: possible role in the decidualization process.

AU - Torricelli, M

AU - De Falco, G

AU - Florio, P

AU - Rossi, M

AU - Leucci, E

AU - Viganò, P

AU - Leoncini, L

AU - Petraglia, F

N1 - Keywords: Adult; Corticotropin-Releasing Hormone; Cyclic AMP; Decidua; Endometrium; Estradiol; Female; Gene Expression; Humans; Medroxyprogesterone 17-Acetate; Menstrual Cycle; Prolactin; RNA, Messenger; Stromal Cells; Urocortins

PY - 2006

Y1 - 2006

N2 - BACKGROUND: Urocortin (UCN) gene expression and synthesis have been reported in epithelial and stromal cells of the human endometrium. In this study we evaluated (i) UCN messenger RNA (mRNA) expression and peptide production in uterine specimens collected throughout the endometrial cycle, (ii) UCN secretion after decidualization of cultured human endometrial stromal cells (HESCs) and (iii) the effect of UCN on endometrial decidualization. METHODS: HESCs were isolated from samples of human endometrium collected from healthy patients with normal menstrual cycle and cultured in presence of cAMP, 17-beta-estradiol (E(2)) + medroxyprogesterone acetate (MPA) and UCN. UCN levels were measured in endometrial extracts by an enzyme immunoassay, and changes of endometrial UCN mRNA expression were measured by RT-PCR analysis. RESULTS: UCN peptide concentrations and mRNA expression were highest in the secretory phase of the menstrual cycle (P < 0.001, late secretory versus early and late proliferative phase) and higher in the late than the early secretory phase (P < 0.01). After decidualization of HESC with cAMP or E(2) + MPA, UCN levels rose in parallel with prolactin concentrations by days 6 (P < 0.01, for all). Finally, the addition of UCN to HESCs, with or without E(2) + MPA, induced the release of prolactin. CONCLUSIONS: The evidence that (i) UCN is highly expressed in the secretory phase of the endometrial cycle; (ii) cAMP and E(2) + MPA modulate secretion of UCN and (iii) UCN induces HESCs decidualization together suggest a possible role for UCN in endometrial physiology.

AB - BACKGROUND: Urocortin (UCN) gene expression and synthesis have been reported in epithelial and stromal cells of the human endometrium. In this study we evaluated (i) UCN messenger RNA (mRNA) expression and peptide production in uterine specimens collected throughout the endometrial cycle, (ii) UCN secretion after decidualization of cultured human endometrial stromal cells (HESCs) and (iii) the effect of UCN on endometrial decidualization. METHODS: HESCs were isolated from samples of human endometrium collected from healthy patients with normal menstrual cycle and cultured in presence of cAMP, 17-beta-estradiol (E(2)) + medroxyprogesterone acetate (MPA) and UCN. UCN levels were measured in endometrial extracts by an enzyme immunoassay, and changes of endometrial UCN mRNA expression were measured by RT-PCR analysis. RESULTS: UCN peptide concentrations and mRNA expression were highest in the secretory phase of the menstrual cycle (P < 0.001, late secretory versus early and late proliferative phase) and higher in the late than the early secretory phase (P < 0.01). After decidualization of HESC with cAMP or E(2) + MPA, UCN levels rose in parallel with prolactin concentrations by days 6 (P < 0.01, for all). Finally, the addition of UCN to HESCs, with or without E(2) + MPA, induced the release of prolactin. CONCLUSIONS: The evidence that (i) UCN is highly expressed in the secretory phase of the endometrial cycle; (ii) cAMP and E(2) + MPA modulate secretion of UCN and (iii) UCN induces HESCs decidualization together suggest a possible role for UCN in endometrial physiology.

U2 - 10.1093/humrep/del331

DO - 10.1093/humrep/del331

M3 - Journal article

C2 - 16920724

SN - 0268-1161

VL - 22

SP - 92

EP - 96

JO - Human Reproduction

JF - Human Reproduction

IS - 1

ER -