Sex- and age-specific reference intervals of 16 steroid metabolites quantified simultaneously by LC-MS/MS in sera from 2458 healthy subjects aged 0 to 77 years

Hanne Frederiksen*, Trine Holm Johannsen, Stine Ehlern Andersen, Jørgen Holm Petersen, Alexander Siegfried Busch, Marie Lindhardt Ljubicic, Margit Bistrup Fischer, Emmie N. Upners, Casper P. Hagen, Katharina M. Main, Lise Aksglaede, Niels Jørgensen, Line Lund Kårhus, Allan Linneberg, Anna Maria Andersson, Christa E. Flück, Anders Juul

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstract

Background
Reference intervals covering the whole life span for all the metabolites in the steroid hormone biosynthesis quantified by sensitive and robust analytical methods are sparse or not existing.

Objective
To develop a state-of-the-art LC-MS/MS method for simultaneous quantification of multiple steroid metabolites and to establish detailed sex- and age-specific reference intervals for 16 steroid metabolites.

Materials and method
An isotope diluted LC-MS/MS method was developed for simultaneous quantitation of 16 steroid hormones. Serum samples from cross-sectional cohorts of healthy infants, children, adolescents, and adults aged 0.17 months to 77 years (n = 2458) were analysed.

Results
With this novel, specific, and sensitive LC-MS/MS method, it was possible to quantify progesterone, 17-hydroxypregnenolone, 17-hydroxyprogesterone, dehydroepiandrosterone sulfate, androstenedione, testosterone, dihydrotestosterone, 11-deoxycorticosterone, corticosterone, 11-deoxycortisol, cortisol, and cortisone in ≥90 % of the samples, while estrone sulfate, aldosterone and dehydroepiandrosterone were quantified in 77 %, 75 % and 60 % of the samples, respectively. 21-deoxycortisol was only detectable in 2.5 % of samples from healthy subjects. Sex- and age-dependent fluctuations observed in minipuberty, puberty and adulthood including the menopausal transition were modelled. This enabled us to establish valid reference intervals from birth to late adult life for both males and females.

Conclusion
Detailed sex- and age-specific reference intervals of multiple, simultaneously quantified steroid metabolites by a novel and specific LC-MS/MS method provides a valuable tool for clinical practice and for future research.
OriginalsprogEngelsk
Artikelnummer119852
TidsskriftClinica Chimica Acta
Vol/bind562
Antal sider10
ISSN0009-8981
DOI
StatusUdgivet - 2024

Bibliografisk note

Publisher Copyright:
© 2024

Citationsformater