Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Nature Biotechnology |
| Vol/bind | 28 |
| Udgave nummer | 5 |
| Sider (fra-til) | 516-20 |
| Antal sider | 4 |
| ISSN | 1087-0156 |
| DOI | |
| Status | Udgivet - 2010 |
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Single base-resolution methylome of the silkworm reveals a sparse epigenomic map. / Xiang, Hui; Zhu, Jingde; Chen, Quan; Dai, Fangyin; Li, Xin; Li, Muwang; Zhang, Hongyu; Zhang, Guojie; Li, Dong; Dong, Yang; Zhao, Li; Lin, Ying; Cheng, Daojun; Yu, Jian; Sun, Jinfeng; Zhou, Xiaoyu; Ma, Kelong; He, Yinghua; Zhao, Yangxing; Guo, Shicheng; Ye, Mingzhi; Guo, Guangwu; Li, Yingrui; Li, Ruiqiang; Zhang, Xiuqing; Ma, Lijia; Kristiansen, Karsten; Guo, Qiuhong; Jiang, Jianhao; Beck, Stephan; Xia, Qingyou; Wang, Wen; Wang, Jun.
I: Nature Biotechnology, Bind 28, Nr. 5, 2010, s. 516-20.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Single base-resolution methylome of the silkworm reveals a sparse epigenomic map
AU - Xiang, Hui
AU - Zhu, Jingde
AU - Chen, Quan
AU - Dai, Fangyin
AU - Li, Xin
AU - Li, Muwang
AU - Zhang, Hongyu
AU - Zhang, Guojie
AU - Li, Dong
AU - Dong, Yang
AU - Zhao, Li
AU - Lin, Ying
AU - Cheng, Daojun
AU - Yu, Jian
AU - Sun, Jinfeng
AU - Zhou, Xiaoyu
AU - Ma, Kelong
AU - He, Yinghua
AU - Zhao, Yangxing
AU - Guo, Shicheng
AU - Ye, Mingzhi
AU - Guo, Guangwu
AU - Li, Yingrui
AU - Li, Ruiqiang
AU - Zhang, Xiuqing
AU - Ma, Lijia
AU - Kristiansen, Karsten
AU - Guo, Qiuhong
AU - Jiang, Jianhao
AU - Beck, Stephan
AU - Xia, Qingyou
AU - Wang, Wen
AU - Wang, Jun
PY - 2010
Y1 - 2010
N2 - Epigenetic regulation in insects may have effects on diverse biological processes. Here we survey the methylome of a model insect, the silkworm Bombyx mori, at single-base resolution using Illumina high-throughput bisulfite sequencing (MethylC-Seq). We conservatively estimate that 0.11% of genomic cytosines are methylcytosines, all of which probably occur in CG dinucleotides. CG methylation is substantially enriched in gene bodies and is positively correlated with gene expression levels, suggesting it has a positive role in gene transcription. We find that transposable elements, promoters and ribosomal DNAs are hypomethylated, but in contrast, genomic loci matching small RNAs in gene bodies are densely methylated. This work contributes to our understanding of epigenetics in insects, and in contrast to previous studies of the highly methylated genomes of Arabidopsis and human, demonstrates a strategy for sequencing the epigenomes of organisms such as insects that have low levels of methylation.
AB - Epigenetic regulation in insects may have effects on diverse biological processes. Here we survey the methylome of a model insect, the silkworm Bombyx mori, at single-base resolution using Illumina high-throughput bisulfite sequencing (MethylC-Seq). We conservatively estimate that 0.11% of genomic cytosines are methylcytosines, all of which probably occur in CG dinucleotides. CG methylation is substantially enriched in gene bodies and is positively correlated with gene expression levels, suggesting it has a positive role in gene transcription. We find that transposable elements, promoters and ribosomal DNAs are hypomethylated, but in contrast, genomic loci matching small RNAs in gene bodies are densely methylated. This work contributes to our understanding of epigenetics in insects, and in contrast to previous studies of the highly methylated genomes of Arabidopsis and human, demonstrates a strategy for sequencing the epigenomes of organisms such as insects that have low levels of methylation.
U2 - 10.1038/nbt.1626
DO - 10.1038/nbt.1626
M3 - Journal article
C2 - 20436463
VL - 28
SP - 516
EP - 520
JO - Nature Biotechnology
JF - Nature Biotechnology
SN - 1087-0156
IS - 5
ER -