@article{31b6965008c611de8478000ea68e967b,
title = "Solid-Phase Enzymatic Synthesis of a Lewis a Trisaccharide Using an Acceptor Reversibly Bound to Sepharose",
abstract = "The disaccharide 2-aminoethyl O-{\ss}-D-galactopyranosyl-(1¿3)-2-acetamido-2-deoxy-{\ss}-D-glucopyranoside was reacted with thiobutyrolactone to give a disaccharide with a thiol group on the aglycone. This disaccharide was reacted with activated Thiopropyl Sepharose, which gave a disaccharide bound to Sepharose via a disulphide bond. Enzymatic fucosylation, using GDP-fucose and partially purified human milk fucosyltransferase, gave a trisaccharide in good yield, which was cleaved from Sepharose by treatment with mercaptoethanol or dithiothreitol.",
author = "Blixt, {Klas Ola} and T. Norberg",
year = "1997",
doi = "10.1080/07328309708006516",
language = "English",
volume = "16",
pages = "143--154",
journal = "Journal of Carbohydrate Chemistry",
issn = "0732-8303",
publisher = "Taylor & Francis",
number = "2",
}