Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Pflügers Archiv: European Journal of Physiology |
| Vol/bind | 453 |
| Udgave nummer | 6 |
| Sider (fra-til) | 777-85 |
| Antal sider | 8 |
| ISSN | 0031-6768 |
| DOI | |
| Status | Udgivet - 2006 |
Bibliografisk note
Keywords: Aquaporin 5; Cystic Fibrosis; Cytoplasmic Vesicles; Female; Fluorescent Antibody Technique; Humans; Hypertonic Solutions; Male; Nasal Mucosa; Nasal Polyps; Osmotic Pressure; Permeability; Spheroids, Cellular; WaterAdgang til dokumentet
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Stimulation of aquaporin-5 and transepithelial water permeability in human airway epithelium by hyperosmotic stress. / Pedersen, Peter Steen; Braunstein, Thomas Hartig; Jørgensen, Anders; Larsen, Per Leganger; Frederiksen, Ole; Holstein-Rathlou, N.-H.
I: Pflügers Archiv: European Journal of Physiology, Bind 453, Nr. 6, 2006, s. 777-85.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Stimulation of aquaporin-5 and transepithelial water permeability in human airway epithelium by hyperosmotic stress.
AU - Pedersen, Peter Steen
AU - Braunstein, Thomas Hartig
AU - Jørgensen, Anders
AU - Larsen, Per Leganger
AU - Frederiksen, Ole
AU - Holstein-Rathlou, N.-H.
N1 - Keywords: Aquaporin 5; Cystic Fibrosis; Cytoplasmic Vesicles; Female; Fluorescent Antibody Technique; Humans; Hypertonic Solutions; Male; Nasal Mucosa; Nasal Polyps; Osmotic Pressure; Permeability; Spheroids, Cellular; Water
PY - 2006
Y1 - 2006
N2 - Osmotic water permeability (P(f )) was measured in spheroid-shaped human nasal airway epithelial explants pre-exposed to increasing levels of hyperosmotic stress. The fluid-filled spheroids, derived from nasal polyps, were lined by a single cell layer with the ciliated apical cell membrane facing the outside. The P(f ) was determined from diameter changes of the spheroids in response to changes in bathing medium osmolarity forth and back between 300 and 225 mOsm x l(-1). Continuous diameter measurements also allowed determination of spontaneous fluid absorption. Hyperosmotic pretreatment (increase from 300 up to 600 mOsm x l(-1)) caused a time- and osmolarity-dependent increase (up to approximately 1.5 times) in epithelial P(f ) which was of similar magnitude in cystic fibrosis (CF) and non-CF spheroids. The effect saturated at approximately 450 mOsm x l(-1) and at approximately 24 h. Expression of aquaporin-5 (AQP5), studied by immunofluorescence and confocal microscopy, showed an increase in parallel with the increase in P(f ) following hyperosmotic stress. The AQP5 was localized both in cytoplasmic vesicles and in apical cell membranes. Spontaneous fluid absorption rates were equal in CF and non-CF spheroids and were not significantly influenced by hyperosmotic stress. The results suggest that hyperosmotic stress is an important activator of AQP-5 in human airway epithelium, leading to significantly increased transepithelial water permeability.
AB - Osmotic water permeability (P(f )) was measured in spheroid-shaped human nasal airway epithelial explants pre-exposed to increasing levels of hyperosmotic stress. The fluid-filled spheroids, derived from nasal polyps, were lined by a single cell layer with the ciliated apical cell membrane facing the outside. The P(f ) was determined from diameter changes of the spheroids in response to changes in bathing medium osmolarity forth and back between 300 and 225 mOsm x l(-1). Continuous diameter measurements also allowed determination of spontaneous fluid absorption. Hyperosmotic pretreatment (increase from 300 up to 600 mOsm x l(-1)) caused a time- and osmolarity-dependent increase (up to approximately 1.5 times) in epithelial P(f ) which was of similar magnitude in cystic fibrosis (CF) and non-CF spheroids. The effect saturated at approximately 450 mOsm x l(-1) and at approximately 24 h. Expression of aquaporin-5 (AQP5), studied by immunofluorescence and confocal microscopy, showed an increase in parallel with the increase in P(f ) following hyperosmotic stress. The AQP5 was localized both in cytoplasmic vesicles and in apical cell membranes. Spontaneous fluid absorption rates were equal in CF and non-CF spheroids and were not significantly influenced by hyperosmotic stress. The results suggest that hyperosmotic stress is an important activator of AQP-5 in human airway epithelium, leading to significantly increased transepithelial water permeability.
U2 - 10.1007/s00424-006-0157-3
DO - 10.1007/s00424-006-0157-3
M3 - Journal article
C2 - 17043812
VL - 453
SP - 777
EP - 785
JO - Pflügers Archiv - European Journal of Physiology
JF - Pflügers Archiv - European Journal of Physiology
SN - 0031-6768
IS - 6
ER -