Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Journal of Endocrinology |
| Vol/bind | 188 |
| Udgave nummer | 3 |
| Sider (fra-til) | 481-92 |
| Antal sider | 11 |
| ISSN | 0022-0795 |
| DOI | |
| Status | Udgivet - 2006 |
Bibliografisk note
Keywords: 1-Phosphatidylinositol 3-Kinase; Adenylate Cyclase; Androstadienes; Animals; Animals, Newborn; Cell Line; Cell Proliferation; Cyclic AMP-Dependent Protein Kinases; Cyclin D1; Enzyme Activation; Flavonoids; Forskolin; Gastric Inhibitory Polypeptide; Glucagon-Like Peptide 1; Human Growth Hormone; Imidazoles; Insulin-Secreting Cells; Isoquinolines; Mitogen-Activated Protein Kinase Kinases; Pyridines; Rats; Signal Transduction; Stimulation, Chemical; Sulfonamides; Transcription, Genetic; Transduction, GeneticAdgang til dokumentet
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I: Journal of Endocrinology, Bind 188, Nr. 3, 2006, s. 481-92.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Stimulation of pancreatic beta-cell replication by incretins involves transcriptional induction of cyclin D1 via multiple signalling pathways.
AU - Friedrichsen, Birgitte N
AU - Neubauer, Nicole
AU - Lee, Ying C
AU - Gram, Vivian K
AU - Blume, Niels
AU - Petersen, Jacob S
AU - Nielsen, Jens H
AU - Møldrup, Annette
N1 - Keywords: 1-Phosphatidylinositol 3-Kinase; Adenylate Cyclase; Androstadienes; Animals; Animals, Newborn; Cell Line; Cell Proliferation; Cyclic AMP-Dependent Protein Kinases; Cyclin D1; Enzyme Activation; Flavonoids; Forskolin; Gastric Inhibitory Polypeptide; Glucagon-Like Peptide 1; Human Growth Hormone; Imidazoles; Insulin-Secreting Cells; Isoquinolines; Mitogen-Activated Protein Kinase Kinases; Pyridines; Rats; Signal Transduction; Stimulation, Chemical; Sulfonamides; Transcription, Genetic; Transduction, Genetic
PY - 2006
Y1 - 2006
N2 - The incretin hormones, glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic peptide (GIP), have been suggested to act as beta-cell growth factors and may therefore be of critical importance for the maintenance of a proper beta-cell mass. We have investigated the molecular mechanism of incretin-induced beta-cell replication in primary monolayer cultures of newborn rat islet cells. GLP-1, GIP and the long-acting GLP-1 derivative, liraglutide, increased beta-cell replication 50-80% at 10-100 nM upon a 24 h stimulus, whereas glucagon at a similar concentration had no significant effect. The stimulatory effect of GLP-1 and GIP was efficiently mimicked by the adenylate cyclase activator, forskolin, at 10 nM (approximately 90% increase) and was additive (approximately 170-250% increase) with the growth response to human growth hormone (hGH), indicating the use of distinct intracellular signalling pathways leading to mitosis by incretins and cytokines, respectively. The response to both GLP-1 and GIP was completely blocked by the protein kinase A (PKA) inhibitor, H89. In addition, the phosphoinositol 3-kinase (PI3K) inhibitor wortmannin and the mitogen-activated protein kinase kinase (MEK) inhibitor PD98059, both inhibited GLP-1- and GIP-stimulated proliferation. The p38 mitogen-activated protein kinase (MAPK) inhibitor, SB203580, had no inhibitory effect on either GLP-1 or GIP stimulated proliferation. Cyclin Ds act as molecular switches for the G0/G1-S phase transition in many cell types and we have previously demonstrated hGH-induced cyclin D2 expression in the insulinoma cell line, INS-1. GLP-1 time-dependently induced the cyclin D1 mRNA and protein levels in INS-1E, whereas the cyclin D2 levels were unaffected. However, minor effect of GLP-1 stimulation was observed on the cyclin D3 mRNA levels. Transient transfection of a cyclin D1 promoter-luciferase reporter construct into islet monolayer cells or INS-1 cells revealed approximately a 2-3 fold increase of transcriptional activity in response to GLP-1 and GIP, and a 4-7 fold increase in response to forskolin. However, treatment of either cell type with hGH had no effect on cyclin D1 promoter activity. The stimulation of the cyclin D1 promoter by GLP-1 was inhibited by H89, wortmannin, and PD98059. We conclude that incretin-induced beta-cell replication is dependent on cAMP/PKA, p42 MAPK and PI3K activities, which may involve transcriptional induction of cyclin D1. GLP-1, GIP and liraglutide may have the potential to increase beta-cell replication in humans which would have significant impact on long-term diabetes treatment.
AB - The incretin hormones, glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic peptide (GIP), have been suggested to act as beta-cell growth factors and may therefore be of critical importance for the maintenance of a proper beta-cell mass. We have investigated the molecular mechanism of incretin-induced beta-cell replication in primary monolayer cultures of newborn rat islet cells. GLP-1, GIP and the long-acting GLP-1 derivative, liraglutide, increased beta-cell replication 50-80% at 10-100 nM upon a 24 h stimulus, whereas glucagon at a similar concentration had no significant effect. The stimulatory effect of GLP-1 and GIP was efficiently mimicked by the adenylate cyclase activator, forskolin, at 10 nM (approximately 90% increase) and was additive (approximately 170-250% increase) with the growth response to human growth hormone (hGH), indicating the use of distinct intracellular signalling pathways leading to mitosis by incretins and cytokines, respectively. The response to both GLP-1 and GIP was completely blocked by the protein kinase A (PKA) inhibitor, H89. In addition, the phosphoinositol 3-kinase (PI3K) inhibitor wortmannin and the mitogen-activated protein kinase kinase (MEK) inhibitor PD98059, both inhibited GLP-1- and GIP-stimulated proliferation. The p38 mitogen-activated protein kinase (MAPK) inhibitor, SB203580, had no inhibitory effect on either GLP-1 or GIP stimulated proliferation. Cyclin Ds act as molecular switches for the G0/G1-S phase transition in many cell types and we have previously demonstrated hGH-induced cyclin D2 expression in the insulinoma cell line, INS-1. GLP-1 time-dependently induced the cyclin D1 mRNA and protein levels in INS-1E, whereas the cyclin D2 levels were unaffected. However, minor effect of GLP-1 stimulation was observed on the cyclin D3 mRNA levels. Transient transfection of a cyclin D1 promoter-luciferase reporter construct into islet monolayer cells or INS-1 cells revealed approximately a 2-3 fold increase of transcriptional activity in response to GLP-1 and GIP, and a 4-7 fold increase in response to forskolin. However, treatment of either cell type with hGH had no effect on cyclin D1 promoter activity. The stimulation of the cyclin D1 promoter by GLP-1 was inhibited by H89, wortmannin, and PD98059. We conclude that incretin-induced beta-cell replication is dependent on cAMP/PKA, p42 MAPK and PI3K activities, which may involve transcriptional induction of cyclin D1. GLP-1, GIP and liraglutide may have the potential to increase beta-cell replication in humans which would have significant impact on long-term diabetes treatment.
U2 - 10.1677/joe.1.06160
DO - 10.1677/joe.1.06160
M3 - Journal article
C2 - 16522728
SN - 0022-0795
VL - 188
SP - 481
EP - 492
JO - Journal of Endocrinology
JF - Journal of Endocrinology
IS - 3
ER -