@article{84c141807da011dd81b0000ea68e967b,
title = "Stimulation of the N-methyl-D-aspartate receptor has a trophic effect on differentiating cerebellar granule cells.",
abstract = "N-methyl-D-aspartate (NMDA) supplementation of cerebellar cultures enriched in granule neurones (about 90%) prevented the extensive cell loss which occurs when cultivation takes place, in serum containing media, in the presence of 'low' K+ (5-15 mM). Estimation of tetanus toxin receptors and N-CAM contents indicated that NMDA rescued primarily nerve cells. The influence of NMDA in promoting cell survival was blocked by the receptor antagonist, 2-amino-5-phosphonovalerate. The effect depended both on the concentration of NMDA and on the degree of depolarization of cells, the affinity in the presence of 15 mM K+ being similar to that of NMDA receptor binding. The results attest a new role for excitatory amino acid transmitters by showing that they can exert a stage-dependent trophic action on developing nerve cells.",
author = "R Bal{\'a}zs and N Hack and J{\o}rgensen, {Ole Steen}",
note = "Keywords: Animals; Antigens, Surface; Aspartic Acid; Cell Adhesion Molecules; Cell Count; Cell Differentiation; Cell Survival; Cells, Cultured; Cerebellum; N-Methylaspartate; Rats; Receptors, N-Methyl-D-Aspartate; Receptors, Neurotransmitter; Tetanus Toxin",
year = "1988",
language = "English",
volume = "87",
pages = "80--6",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1-2",
}